BIOLASE DNA Polymerase with High Precision Superiority

National Dairy Research Institute
Animal Biochemistry
Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Bioline, UK

Product Name:

BIOLASETM DNA polymerase

Catalog Number:

BIO-21042

DNA can be amplified by using BIOLASETM DNA polymerase which possess 5’-3’ exonuclease activity along with polymerase activity. It produces the PCR amplified products with high precision.

Experimental Design and Results Summary

Application

DNA amplification and the PCR products can be integrated into cloning vectors.

Starting Material

DNA

Protocol Overview

DNA was amplified by using BIOLASETM DNA polymerase. Thie kit also contain 10x NH4 Reaction buffer and 50mM MgCl2 solution. DNA template and primers were added and further PCR machine was programmed to amplify the DNA.

Tips

Kit should be stored at -20°C

Results Summary

The amplified DNA was run on the agarose gel and sharp bands were observed and there was no non-specific amplification

Additional Notes

None

Image Gallery

Summary

The Good

It produces the PCR amplified products with high precision and these DNA products can directly be cloned into cloning vectors

The Bad

None

The Bottom Line

DNA amplification and cloning is made easy BIOLASETM DNA polymerase

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