384 Well Gene Expression Assays

Boston University
Center for Regenerative Medicine
Postdoctoral Associate

Overall

Performance

Ease-of-Optimization

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Company:

Thermofisher, Life Technologies, Applied Biosystems

Product Name:

QuantStudio 6 Flex

Catalog Number:

4485694

The aim of this experiment was to quantify multiple genes in a 384-well plate format.

Experimental Design and Results Summary

Application

Gene Expression

Starting Material

cDNA

Protocol Overview

384-well plates were used. Multichannel pipettes for adding the reagents and cDNA to each well.

Tips

Use multichannel pipettes

Results Summary

Gene expression results were generated. The comparative delta delta Ct method was used.

Features Summary

Allows multiple genes to be investigated at the same time.

Additional Notes

None

Image Gallery

Summary

The Good

Convenient

The Bad

Difficult to visualize the contents in a plate.

The Bottom Line

Very convenient for studying multiple genes and samples in one run.

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