Thermo Fisher, Life Technologies, Applied Biosystems
Step-One-Plus Real-Time PCR
4376600
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The aim of this experiment was to measure the relative gene expression levels of multiple samples using the Step-One-Plus Real-Time PCR machine.
Gene Expression
cDNA
The PCR machine was used to quantify the relative gene expression levels. The StepOnePlus software is very intuitive and easy to use. The Fast protocol was used in this run.
Run your plate prior to labelling each well and assigning probes- saves time
The fast protocol allowed the RT-qPCR to be completed in 45 minutes. The threshold levels for each gene were automatically derived by the software. The comparative delta delta Ct method setting also calculated the mean delta Ct values for each of the samples.
Intuitive software, efficient and easy to use
None
Ease of use
A great upgrade of the previous qPCR machines.
Product Name: StepOnePlus ™ Real-Time PCR SystemSupplier Page