RNA Purification

National Institute of Nutrition
Biochemistry
Senior research fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Qiagen

Product Name:

RNeasy Mini Kit

Catalog Number:

74104

This kit is used for purification of total RNA from animal cells and animal tissues and for cleanup of RNA from crude RNA preparations and enzymatic reactions (e.g., DNase digestion, proteinase digestion, RNA ligation, and labeling reaction).

Experimental Design and Results Summary

Application

Real time PCR analysis.

Starting Material

Rat tissue samples.

Protocol Overview

I used this kit to separate RNA from rat tissue. Take the snap frozen tissue and homogenize. Put 400 µl of tissue lysate in columns. Then follow the manufactures’ instructions.

Tips

Use for properly storing samples.

Results Summary

Got good purity of RNA

Additional Notes

Carefully take out the supernatant.

Image Gallery

Summary

The Good

Simple steps

The Bad

Columns are costly.

The Bottom Line

Simple and good

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