Anti-GTF2F1 Immunofluorescence

Tumor Biology
German Cancer Research Center
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Atlas Antibodies

Product Name:

Anti-GTF2F1

Catalog Number:

HPA022793

The main goal of our research is to identify new genes that play critical roles in cancer formation and development. To characterize the candidate genes and examine their cellular functions, we perform several cell-based assays. Having a specific antibody that is able to detect the target protein is a fundamental point in our experimental model.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Caco-2 cells

Primary Incubation

1:20 diluted in 1xPBS, incubation for 1h at room temperature

Blocking Agent

No blocking

Secondary Incubation

Cy3-coupled secondary antibody, 30 min incubation at room temperature

Tertiary Incubation

NA

Detection

Fluorescence microscopy

Results Summary

Specific nuclear staining in Caco-2 colon carcinoma cells

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Specific staining

The Bad

Too low dilution

The Bottom Line

Good antibody for the detection of the GTF2F1 protein in cells using the immunofluorescence method.

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