BioLegend
Brilliant Violet 421™ anti-mouse/human CD45R/B220
103239
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We have an immunology lab that studies the phenotypes of B cells in mice. This antibody is very effective at labeling B cells from total splenocyte preparations.
Flow Cytometry
Live murine splenocytes, red blood cells lysed
1:100 Brilliant Violet 421™ anti-mouse B220 on ice for 10 minutes.
1x PBS + 2% FBS + 2mM EDTA
NA
BD LSR Fortessa
Splenic B cells are strongly and specifically labelled with BV421.
None
Reliable results, easy to distinguish B220+ from B220- cells.
No drawbacks observed.
I would recommend this product to anyone. It gives bright, consistent labeling of B cells.