Jackson ImmunoResearch Laboratories, Inc.
Rabbit IgG FITC
313-096-003
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The aim of this experiment was to determine the proportion of RBC'S bound to different immunoglobulins from dogs that have immune mediated hemolytic anemia. This antibody was used as an isotope control for the Rabbit anti dog IgG antibody which is used to detect bound RBC to IgG.
Flow Cytometry
Whole blood in EDTA
100ul of 1:100diluted 1mg/ml antibody with the 100ul of washed cells incubated for 30mins in dark.
NA
FACS CAlibur
The results for this particular antibody helps us to determine the negative population of cells that did not stain. Hence it serves as a good isotype control.
None
Works well as an Isotype
Good control.