Bethyl Lab
Goat anti dog complement 3 (C3) FITC
A40-109F
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The aim of this experiment was to determine the proportion o RBC'S bound to the complement 3 of the complement system from dogs that have immune mediated hemolytic anemia. This antibody serves the purpose of detecting such bound RBC's.
Flow Cytometry
Whole blood in EDTA
100ul of 1:100diluted 1mg/ml antibody with the 100ul of washed cells incubated for 30mins in dark.
NA
FACS CAlibur
The results generated by flow cytometry are in the form of percent positive or negative. In the case of dogs that are suspected of IMHA, we do see a positive population of cells bound to the complement 3 component. The percentage of these cells varies from dog to dog.
None
Works well for our antibodies
Good antibody.