Very Good STAT3 Antibody

Neurosurgery
Havard medical school
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technologies

Product Name:

Stat3 (79D7)

Catalog Number:

4904

I am investigating the levels of phospho-STAT3 in different cancerous cell lines. I needed a good total STAT3 control.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysates

Primary Incubation

4°C, overnight, 1:1000 in 5% BSA in TBST

Blocking Agent

RT, 1h, 5% milk in TBST

Secondary Incubation

RT, 1h, 5% milk in TBST, 1:5000

Tertiary Incubation

NA

Detection

Bio-Rad clarity

Results Summary

Good total STAT3 antibody. Works well with the phospho-STAT3 from CST

Additional Notes

None

Related Categories

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Summary

The Good

Easy to use

The Bad

None

The Bottom Line

Very good STAT3 antibody

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