Cell Signaling Technology
PCAF (C14G9) Rabbit mAb #3378
3378
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Browse Similar Products: PCAF Western blot Antibodies
We were screening PCAF knockout candidates in mouse embryonic stem cells. We chose the antibody because the band on the image of Western blot on the antibody site looked specific and of the right size.
Western Blot
Whole cell lysates from mouse ES cells
1:1000 dilution overnight in 5% bsa at 4C
5% bsa
1:3000 antirabbit secondary at RT for 1 hour
NA
Exposure with SuperSignal™ West Femto Maximum Sensitivity Substrate, imaged with apparatus from BioRad
Band was very clear and of expected size. Great antibody!
None
Band is clear and specific
Great antibody!