Premixed and Ready to Use Solution for Gene Amplification

January 05, 2016

BlueskyReddit
National Dairy Research Institute
Animal Biochemistry
Scientist

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Company:

Sigma Aldrich, USA

Product Name:

ReadyMixTM Taq PCR Reaction Mix with MgCl2

Catalog Number:

P4600-100RXN

For gene expression studies, RNA was isolated and converted into cDNA by kit based method. This cDNA and specific primers were mixed with Taq ® Master Mix for amplification by PCR.

Experimental Design and Results Summary

Application

Gene amplification

Starting Material

cDNA

Protocol Overview

ReadyMixTM Taq PCR Reaction Mix with MgCl2 is a prepared solution which contains everything in proper quantity needed for gene amplification by PCR. cDNA and the specific primers are added to the reaction mix according to the manufacturer’s instructions and the PCR programme was set up and run. Once the PCR was over, the amplified product was observed in 3% agarose gel.

Tips

None

Results Summary

The quality of bands were very good when observed in 3% agarose gel

Additional Notes

None

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Summary

The Good

This premixed solution reduces the amount of pipetting required for different components

The Bad

Amplified product cannot be seen in agarose gel without any DNA binding dye.

The Bottom Line

ReadyMixTM Taq PCR Reaction Mix with MgCl2 is best for DNA amplification.

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