Antibodies-online
Anti-Carboxypeptidase X (M14 Family), Member 2 (CPXM2) (N-Term) antibody from antibodies-online
ABIN452869
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We performed protein expression analyses in human podocytes regarding the generation of different factors involved in apoptosis regulation, signaling, cell structure maintenance and cell viability. Specifically phospho-specific antibodies were used to analyse the signaling activity normalized againt total protein expression (see Akt, AMPK and mTOR). We performed Western blot analyses of CPXM2 protein expression in cell lysates of human PBMC using the anti CPXM2(N-Term) antibody from Antibodies-Online.
Western Blot
Cell lysates of human PBMC
Dilution 1:500, 16 overnight, 4°C
5% milk powder in TBST buffer
Dilution: 1:5000, 1 hour, 24°C
ECL - 20 min, room temperature
ECL
Different bands were detected after 20 min including a CPXM2-band at 86-90 kDa (see figure, arrow).
None
A band including CPXM2 (86-90 kDa) was detected after 20 min (black arrow)
Some other bands were detected, too
This antibody is ok for Western blot analyses of CPXM2