Western Blot Using Anti CPXM2(N-Term)

Klin. Pharmakol. und Tox.
Charite-Berlin
PhD

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Company:

Antibodies-online

Product Name:

Anti-Carboxypeptidase X (M14 Family), Member 2 (CPXM2) (N-Term) antibody from antibodies-online

Catalog Number:

ABIN452869

We performed protein expression analyses in human podocytes regarding the generation of different factors involved in apoptosis regulation, signaling, cell structure maintenance and cell viability. Specifically phospho-specific antibodies were used to analyse the signaling activity normalized againt total protein expression (see Akt, AMPK and mTOR). We performed Western blot analyses of CPXM2 protein expression in cell lysates of human PBMC using the anti CPXM2(N-Term) antibody from Antibodies-Online.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysates of human PBMC

Primary Incubation

Dilution 1:500, 16 overnight, 4°C

Blocking Agent

5% milk powder in TBST buffer

Secondary Incubation

Dilution: 1:5000, 1 hour, 24°C

Tertiary Incubation

ECL - 20 min, room temperature

Detection

ECL

Results Summary

Different bands were detected after 20 min including a CPXM2-band at 86-90 kDa (see figure, arrow).

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

A band including CPXM2 (86-90 kDa) was detected after 20 min (black arrow)

The Bad

Some other bands were detected, too

The Bottom Line

This antibody is ok for Western blot analyses of CPXM2

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