CD3 PE Antibody Review

Microbiology
Medical College of Wisconsin
Research Technologist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

CD3 PE Antibody

Catalog Number:

100205

Splenocytes from latently infected mice are analyzed for various immune cells (T and B cells) and those are quantified at various time points of latent infection.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes

Primary Incubation

1:100, 10 minutes

Blocking Agent

Fc Block

Secondary Incubation

1:1600, 20 minutes

Tertiary Incubation

NA

Detection

LSRII

Results Summary

We use this antibody in panels with 3-4 other stains usually. We are able to clearly identify the populations of interest and quantify the changes in T and B cell numbers at various stages of latency via flow cytometry.

Additional Notes

None

Related Categories

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Summary

The Good

Good signal

The Bad

NA

The Bottom Line

Good antibody, would recommend it if you need to use PE for identifying CD3+ cells.

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