CD5-APC Antibody Review

November 30, 2015

Microbiology
Medical College of Wisconsin
Research Technologist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

CD5 APC Antibody

Catalog Number:

100625

Splenocytes from latently infected mice are collected, processed, and stained for various immune cell markers to identify different populations within the spleen.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes

Primary Incubation

1:100, 10 minutes

Blocking Agent

Fc Block

Secondary Incubation

1:800

Tertiary Incubation

NA

Detection

LSRII

Results Summary

T and B Cells are identified within the lymphocyte population of the spleen.

Additional Notes

We have also used this antibody for sorting of B cell population subsets and it has worked well.

Related Categories

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Summary

The Good

Reliable, Versatile Antibody

The Bad

NA

The Bottom Line

Good antibody, we use it for many of our flow cytometry and cell sorting experiments.

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