CD4 Pacific Blue Antibody Review

November 25, 2015

Microbiology
Medical College of Wisconsin
Research Technologist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

CD4 Pacific Blue Antibody

Catalog Number:

100531

Spleens from latently infected mice are collected and processed for flow. Identification and quantification of various immune cells are then established.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes

Primary Incubation

1:100, 10 minutes

Blocking Agent

Fc Block

Secondary Incubation

1:1600, 20 minutes

Tertiary Incubation

NA

Detection

LSRII

Results Summary

T cells, B cells, and granulocytes are identified and quantified. We are looking at changes in cell populations at various time points of latency.

Additional Notes

None

Image Gallery

Summary

The Good

Strong signal

The Bad

NA

The Bottom Line

Great antibody for anyone needing to identify CD4+ cells in their populations.

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