Good CD19 Antibody

Microbiology
Medical College of Wisconsin
Research Technologist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

CD19 Pacific Blue Antibody Review

Catalog Number:

115523

This product was used while trying to distinguish B cell populations in the peritoneum of latently infected mice. We selected this product because of a recommendation from another lab.

Experimental Design and Results Summary

Applications

Cell Isolation/Separation

Sample

Peritoneal Exudate Cells

Primary Incubation

1:100 for 15 minutes

Blocking Agent

Fc Block

Secondary Incubation

1:100, 15 minutes

Tertiary Incubation

NA

Detection

Facs Aria

Results Summary

We were effectively able to distinguish between B1, B2, and non B cell populations.

Additional Notes

None

Related Categories

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Summary

The Good

Reliable, Works well

The Bad

Concentrated dilution needed

The Bottom Line

Great antibody, would recommend it definitely for sorting and also for flow cytometry.

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