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Brilliant violet 650 anti-mouse CD8
100742
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CD8 is expressed on MHC-I restricted T cells. Since this is a lineage marker, I wanted to see if it is easily detected by a weak fluorophore so I used BV650 conjugated CD8a antibody. The staining worked perfectly and even with other adjacent channels active it was easy to compensate. Even better was that CD8 expression is quite high, so even the weak fluorphore shows bright fluorescence.
Flow Cytometry
Lymphocytes
1:200 in PBS for 30 minutes
FcR block
NA
Flow Cytometry - BV650 channel
There are two very distinct positive and negative populations; the frequency of positives was physiological and the fluorescence was quite bright with minimal background in the negative population.
None
Easy to use and compensate
Good antibody choice especially for lineage markers when you have a large panel set.