NGS Kit

UNMC
GCBA
Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Agilent Technologies

Product Name:

Sureselect XT2 Human all Exon V5+UTR

Catalog Number:

5190-6221

My lab looks for genetic mutations that cause familiar breast cancer. To find these mutations, I sequence familiar breast cancer patients' exomes.

Experimental Design and Results Summary

Application

Capturing exons

Starting Material

Human DNA

Protocol Overview

The kit only comes with the probes to bind to the DNA library. The kit to make the library and the beads used by this kit are sold separately.

Tips

Test the evaporation rate of the 96 well plate before using this kit

Results Summary

Results were as expected. The image shows a bioanalyzer picture of a DNA library captured using this exome capture kit.  

Additional Notes

None

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Summary

The Good

Agilent sent the kit very quickly

The Bad

The other kits and beads are not sold with this kit.

The Bottom Line

This kit is fine but make sure you can afford all of the other components before buying this kit.

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