Great antibody for detecting human CD42b

Pathology
Brigham and Women's Hospital
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC anti-human CD42b Antibody

Catalog Number:

303912

Info: View Product View Product Specs

Browse Similar Products: CD42b Antibodies

This antibody was used to select and gate on human platelets that were isolated by a method of centrifugation and washing from a sample of peripheral human blood. After purification, the cells were stained with this anti-CD42b mAb for 45 minutes at 4 degree C followed by analysis on a flow cytometer. About 40% purity was observed in this assay and it was easy to differentiate the platelet population from the background. I would highly recommend this antibody for selecting the platelet population from a preparation of washed platelets and for measuring human CD42b expression.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Washed human platelets

Primary Incubation

1:100, 45 minutes at 4 degrees C

Blocking Agent

Buffer with BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

NA

Results Summary

About 40% of cells show positive expression after purification. Easy to detect platelet population as seen in the figure.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Good specificity, low concentration needed

The Bad

None

The Bottom Line

Great antibody for a good price. Highly recommended.

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