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APC anti-human CD42b Antibody
303912
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This antibody was used to select and gate on human platelets that were isolated by a method of centrifugation and washing from a sample of peripheral human blood. After purification, the cells were stained with this anti-CD42b mAb for 45 minutes at 4 degree C followed by analysis on a flow cytometer. About 40% purity was observed in this assay and it was easy to differentiate the platelet population from the background. I would highly recommend this antibody for selecting the platelet population from a preparation of washed platelets and for measuring human CD42b expression.
Flow Cytometry
Washed human platelets
1:100, 45 minutes at 4 degrees C
Buffer with BSA
NA
About 40% of cells show positive expression after purification. Easy to detect platelet population as seen in the figure.
None
Good specificity, low concentration needed
Great antibody for a good price. Highly recommended.