Very Good Anti-Human CD45 antibody

Dept of biomedical Sciences
TTUHSC El Paso
Post doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC anti-human CD45 Antibody

Catalog Number:

304012

Single cell suspensions prepared from the spleen of human PBMC reconstituted mice was used for flow cytometric analysis. I have used this antibody to separate human cells from the spleen of these humanized mice. The cells were stained with this CD45 antibody and analyzed by flow cytometry. The staining looks pretty good with two separate populations of CD45+ and CD45- cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes from humanized mice

Primary Incubation

30 minutes

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry on APC Channel

Results Summary

The results in the figure show a clear positive and negative population for expression of surface CD45.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Great specificity

The Bad

1 ul is enough for each sample instead of 5 ul suggested by company

The Bottom Line

Very good and reliable antibody

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