Good loading control for immunoblotting

Pharmacology
Boston University
Senior Post-doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

EMD Millipore

Product Name:

Anti-GAPDH

Catalog Number:

ABS16

For immunoblotting, a good loading control is always as important as proteins that we want to analyze. GAPDH, tubulin, and actin are the common loading controls used for immunoblotting. The anti-GAPDH from Millipore is specific and sensitive for immunoblotting, making it a great control antibody for researchers.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HeLa human cancer cell line

Primary Incubation

Dilution 1:10000 in 5% non-fat milk/TBS-Tween20. Two hour incubation at room temperature.

Blocking Agent

5% non-fat milk/TBS-Tween20

Secondary Incubation

HRP-conjugated anti-rabbit IgG

Tertiary Incubation

N/A

Detection

Bio-Rad ChemiDoc imager

Results Summary

Using a dilution of 1:10K and incubated for just 1 hour at room temperature, this antibody is still strong enough to get great signal.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Superb specificity and sensitivity

The Bad

None

The Bottom Line

Good antibody. No need to use high concentration of antibody or long exposure of blots.

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