LC3B (D11) XP ® Rabbit mAb 3868S from Cell Signaling Technology

Product Specs
ItemLC3B (D11) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $371.00 Inquire
Catalog Number3868S
Quantity100 µl
ApplicationsW, IF-IC, FC-FP
ReactivityH
TargetLC3B
HostRabbit
NCBI Gene AliasesATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a
ClonalityMonoclonal
NCBI Full Gene Namemicrotubule associated protein 1 light chain 3 beta

Questions and Answers

Can this antibody be used to study autophagy in cancer cells?

Yes, the LC3B (D11) XP Rabbit mAb has been shown to be effective in studying autophagy in various cancer contexts. For instance, it was utilized in research demonstrating that niclosamide-induced inhibition of Wnt signaling in colorectal cancer is mediated through autophagy, with LC3B serving as a key marker for autophagosome formation (Wang et al., 2019).

Is this antibody suitable for use in flow cytometry applications?

The LC3B (D11) XP Rabbit mAb has been cited in studies that employed flow cytometry to analyze autophagy-related processes. For example, it was used to assess the expression of autophagy-related proteins in PC12 cells, indicating its applicability in flow cytometry (Li et al., 2017).

How has this antibody been utilized in studies involving pediatric leukemia?

This antibody has been referenced in research targeting autophagy as a therapeutic strategy in pediatric acute lymphoblastic leukemia. The study highlighted the upregulation of autophagy-related proteins, including LC3B, in leukemic cells, suggesting that this antibody can be instrumental in understanding autophagy's role in leukemia treatment (Bwanika et al., 2024).

What is the significance of using this antibody in immunoblotting experiments?

The LC3B (D11) XP Rabbit mAb is frequently used in immunoblotting to quantify the levels of LC3B-II, a marker of autophagy. Its application in various studies has demonstrated its reliability in detecting changes in autophagy-related protein levels, which is crucial for understanding cellular responses to different treatments (Kang et al., 2017; Yu et al., 2019).

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Website: Technical Support

(1) Glutamine transporter SLC1A5 inhibits autophagy-mediated CD276 degradation to promote esophageal cancer progressionCancer Biology & TherapyDecember 31, 2026Chunyan Wang, Hongyan Zhang, Chaonan Guan, Yuying Li, Shengli Yang, Lan Huangmanufacturer-validated), SLC1A5 (#8057, CST, manufacturer-validated), p62 (#88588, CST, manufacturer-validated), LC3II (#3868, CST, manufacturer-validated), and GAPDH (#2118, CST, manufacturer-validated). After washing, HRP-conjugated

(2) Discovery of N8: a novel IKKε inhibitor with potent anticancer activity via cytotoxicity, migration suppression, and autophagy modulationJournal of Enzyme Inhibition and Medicinal ChemistryDecember 31, 2026Wei Ye, Siying Zheng, Hongmei Xie, Xinrui Zhou, Jiapeng Xu, Qiting Luo, Yuanyuan Huang, Jieyu Li, Jiayi Diao, Xinyi Luo, et al.proteins was determined by Western blotting. Antibodies targeting the following proteins were used: LC3B (3868S, CST), p62 (PM045, MBL), caspase-3 (9662S, CST), cleaved-caspase-8 (8592 T, CST). Annexin V-FITC/PI

(3) TFEB confers resistance against the chemotherapeutic agent CX-5461Autophagy ReportsDecember 31, 2026Marjorie Rolland, Benoît Marchand, Laure Bessy, Florence McDuff, Marie-Josée Boucher(Cell Signaling Technology, 9718), HISTONE H3 (Cell Signaling Technology, 3638), LC3B (Cell Signaling Technology, 3868), TFEB (Cell Signaling Technology, 4240), and TFE3 (Cell Signaling Technology, 81,744). Subsequently,

(4) Pharmacological inhibition of PGK1 suppresses EGFR-positive esophageal squamous cell carcinoma by dual targeting of glycolysis and autophagy-dependent EGFR degradationCell Oncol (Dordr)April 29, 2026Juan Ge, Yongfei Chen, Zongru Jiang, Shuang Qi, Jie Hu, Beilei Wang, Aoli Wang, Qingsong Liu, Hong Wu, Wenchao Wang, et al.Phospho-mTOR(Ser2448) rabbit mAb(#2971), mTOR rabbit mAb (#2972), GAPDH rabbit mAb (#5174), and LC3B rabbit mAb (#3868) were obtained from Cell Signaling Technology (Danvers, MA, USA). PRAS40 rabbit mAb (#21097-1-AP), Phospho-PRAS40

(5) Pathogenesis of Noonan Syndrome is Modulated by NOC2L, a Novel Interactor of LZTR1 Leading to Impaired P53 SignallingThe Journal of Clinical Endocrinology & MetabolismApril 22, 2026Sumana Chatterjee, Miho Ishida, Débora R Bertola, Juliana Chizo Agwu, Carles Gaston-Massuet, Liam J McGuffin, Helen L Storr, Avinaash V MaharajCat# 9206, RRID:AB_2285392), Rabbit monoclonal LC3B (D11) antibody (Cell Signaling Technology Cat# 3868, RRID:AB_2137707), Rabbit anti-GAPDH antibody (ab9485, RRID:AB_307275), Mouse anti-beta Actin monoclonal

(6) ADAMTS2 Modulates Inflammation, Autophagy, and Barrier Integrity Through PI3K/AKT/mTOR Signaling in LPS-Induced Acute Respiratory Distress SyndromeImmun Inflamm DisApril 20, 2026Song Gao, Ting Lyu, Youhong Quan, Yaling Tao, Wenjin Wang, Xi Xunumber,Manufacturer ADAMTS2,Rabbit,1:1000,ab187839,Abcam Occludin,Rabbit,1:1000,27260‐1‐AP,Proteintech ZO‐1,Rabbit,1:1000,21773‐1‐AP,Proteintech LC3B,Rabbit,1:1000,3868S,Cell signaling technology (CST) Beclin‐1,Rabbit,1:1000,ab207612,Abcam p62/SQSTM1,Rabbit,1:1000,ab109012,Abcam PI3K

(7) Generalizable, high-throughput image analysis of subcellular structures using dispersion indicesiScienceApril 17, 2026Andrew Martin, Sue Zhang, Amanda Williamson, Brett Tingley, Mira Pickus, David Zurakowski, Hadi T. Nia, Orian Shirihai, Xue Han, Mark W. Grinstaff12741, RRID:AB_2617131" "Rabit Anti-LC3B, D11 Clone","Cell Signaling Technology, RRID:SCR_002071","Cat# 3868, RRID:AB_2137707" Goat Anti Rabbit AlexaFluor 488,"Jackson ImmunoResearch, RRID:SCR_010488","Cat# 111-545-144,

(8) Autophagy selectively clears ER in TNF-α-induced muscle atrophyAutophagy RepApril 17, 2026Ursula K. Dueren, Alan An Jung Wei, A. Elisabeth Gressler, Simon Rapp, Oliver Popp, Robert Kerridge, Viviana Buonomo, Paolo Grumati, Philipp Mertins, Matthias Selbach, et al.4°C with primary antibodies against LC3B, p62/Sqstm1, and Vinculin (all Cell Signaling Technology, 3868S, 39749S, 13901S; all 1:1000). After washing, the membranes were incubated with HRP-conjugated secondary

(9) Interference of Large Clostridial Glucosyltransferases with the Endolysosomal Pathway: Toxin-Induced Imbalance of Early Endosomes, Functional Lysosomes and AutophagosomesToxins (Basel)April 15, 2026Anna Langejürgen, Gudula Schmidt, Leon Unsöld, Helma Tatge, Ethel Oyson, Ralf GerhardPlanegg-Martinsried, German); anti-Cathepsin D (IF/WB; #6985), anti-EEA1 (IF;2411), anti-LC3B (IF/WB; 3868), anti-SQSTM1 (IF/WB; #88588), anti-Rab7 (IF/WB; 95746), and anti-GAPDH (WB;2118) were all from Cell Signaling Technology

(10) Saikosaponin D Is Associated with Anti-Tumor Effects and Markers of Autophagy and Endoplasmic Reticulum Stress in Human Endometrial Cancer Ishikawa CellsNutrientsApril 13, 2026Xiu-Xiu Zhang, Tong-Tong Tang, Xiao-Mei Ma, Kiran Thakur, Fei Hu, Jian-Guo Zhang, Yi-Long Ma, Zhao-Jun Weionto SDS-PAGE gels. The primary antibodies used were as follows: LC3B (Cell Signaling Technology, #3868, 1:1000), p62/SQSTM1 (Cell Signaling Technology, #8025, 1:1000, Danvers, MA, USA), PERK (Cell Signaling Technology

(11) Gandouling protects against hepatic fibrosis in Wilson disease through the lncRNA-SNHG7/miR-29b/DNMT3A pathway3 BiotechApril 1, 2026Han Wang, Lanting Sun, Xin Yin, Daiping Hua, Qiaoyu Xuan, Jiajia Wang, Wei Dong, Wenming YangBeclin-1 (Abcam, Item No. ab210498, 1:2000, Rabbit; No. ab62472, 1:1000, Rabbit), LC3B (CST, Item No. 3868s, 1:1000, Rabbit; No. 43566 S, 1:1000, Rabbit), and p62 (Bioss, Item No. bs-2951R, 1:1000, Rabbit) at

(12) Corylin inhibits liver cancer cell growth through the endogenous apoptosis and mitophagyTranslational Cancer ResearchMarch 31, 2026Renshuang Zhao, Xin Wang, Mingming Cao, Yaru Li, Shanzhi Li, Zhiru Xiu, Yilong Zhu, Yiquan Lipolymerase (PARP) (Cell Signaling; 5942), anti-CytC (Cell Signaling; 11940), anti-LC3 (Cell Signaling; 3868), anti-p62 (Cell Signaling; 39749), anti-Beclin-1 (Cell Signaling; 3495), anti-PINK1 (Cell Signaling;

(13) Activation of the protective arm of renin-angiotensin system enhances mitochondrial turnover improving respiration and decreasing integrated stress response in a human Complex III deficiency modelbioRxivMarch 27, 2026Lucía Fernández-del-Río, Andrea Eastes, David Rincón Fernández Rincón Fernández Pacheco, Nikole Scillitani, Jasmine Garza, Matthew Dugan, Matheus Pinto Pinto de Oliveira, Pradnya Kadam, Iman Gauhar, Karel Erion, et al.(Santa Cruz Biotechnology, sc-390453); anti-GST-Tag (Cell Signaling, 2624); anti-LC3B (Cell Signaling, 3868); anti-CHOP (Cell Signaling, 2895); anti-LAMIN A/C (Santa Cruz Bio, sc-20681); anti-p3 TUBULIN (Cell Signaling

(14) MIZ-1 controls transcriptional programs required for BCR signaling, actin dynamics, and naïve B cell survivalFrontiers in ImmunologyMarch 27, 2026Julie Ross, Eva-Maria Piskor, Charles Joly-Beauparlant, Lucia Paola Gabrielli Tabarez, Elie El Ghorayeb, Arnaud Droit, Christian Kosan, Tarik Möröy#A21426) alone or in combination with Rab5 (1:200, Cell Signaling #3547T) or LC3B (1:200, Cell Signaling #3868P) in blocking solution overnight at 4 °C. Next day, slides washed 3x with PBS with 0.3% Triton-100. For

(15) Baloxavir Acid-Induced Mitochondrial Toxicity and Cell Cycle Arrest Contribute to Its Adverse EffectsInternational Journal of Molecular SciencesMarch 25, 2026Pengyu Zhan, Yuxing Ren, Kai Han, Guoming Jin, Yang Yang, Lei Shi, Yali CiSuzhou, Jiangsu, China, B1029; Selleck, Houston, TX, USA, F0082), LC3B (D11) (Cell Signaling Technology, #3868), Bak (D2D3) (Cell Signaling Technology, #6947), Cleaved Caspase-3 (Asp175) (Cell Signaling Technology,

(16) Myofibrillar protein accumulation but reduced protein synthesis in PDCD4-depleted myotubesPLoS OneMarch 19, 2026Stephen Mora, Lilia Alihemmat, Logan D. Davari, Termeh Ataie, Parsa Nafari Mehranjani, Luke Flewwelling, Arthur J. Cheng, Olasunkanmi A. J. Adegoke(#9535), p62 (#5114), beclin-1 (#3738), microtubule-associated proteins 1A/1B light chain 3B (LC3B) (#3868), phosphorylated (p) FoxO3aser253 (#9466), p-AKTSer473 (#4060), p-S6Ser235/236 (#4858), and its kinase

(17) Development of Au x Cu y Pd z Nanocomposites as Therapeutic Agents: Enhancing Cancer Treatment through Autophagy Modulation and Immune-Associated EffectsACS Appl Mater InterfacesMarch 18, 2026Li-Xing Yang, Yi-Chun Chiu, Yi-Lun Chen, Ting-Ying Chen, Yi-Tseng Tsai, Yu-Cheng Chin, Ya-Ling Yeh, Ying-Jan Wang, Chih-Chia Huang, Rong-Jane Chen, et al.#A0674, ABclonal, Inc.), anti-GPX4 (cat no. #ab125066, 1:3000 dilution, Abcam plc.), LC3B (cat no. #3868, 1:3000 dilutions, Cell Signaling Technology, Inc.), anti-IDO1 (cat no. #GTX634652, 1:1000 dilutions,

(18) Heart-Specific and Conditional Deletion of the Immt Gene Reveals Its Role in Regulating Mitochondrial Structure and Total Heart MetabolismCellsMarch 12, 2026Yasuhide Kuwabara, Caitlin Keezer, Suh-Chin J. Lin, Akanksha Rajput, Jeffery D. MolkentinProteintech, 1:1000), anti-PINK1 (#BC100-494, Novus Biologicals, Centennial, CO, USA, 1:1000), anti-LC3 (#3868, Cell Signaling Technology, Danvers, MA, USA, 1:1000), anti-p62/SQSTM1 (#P0067, Sigma Aldrich, 1:1000),

(19) Impaired \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\alpha$$\end{document}α-Synuclein aggregate clearance in neuronal cells drive their spread to microglia through tunneling nanotubesNature CommunicationsMarch 12, 2026Ranabir Chakraborty, Francesca Palese, Philippa Samella, Veronica Testa, Jara Montero-Muñoz, Sylvie Syan, Takashi Nonaka, Masato Hasegawa, Antonella Consiglio, Chiara Zurzolomembranes were blocked with 5% BSA and incubated overnight with primary antibodies (anti-LC3B, D11 XP #3868, Cell Signaling Technology, 1:1000; anti-phospho S211-TFEB, E9S8N #37681, Cell Signaling Technology,

(20) Legionella Lem26 functions as an ATG8-activated effector that inhibits host autophagymBioMarch 11, 2026Kevin R. Parducho, Zi Yang, Emily Guinn, Daniel Choi, Shanta Nag, Thomas J. Melia, Craig R. RoyBiolabs TP401, 1:5,000), anti-p62 lck ligand (BD Biosciences 610832, 1:1,000), anti-LC3B (Cell Signaling 3868S, 1:1,000), anti-actinin (BD Biosciences 612576, 1:10,000), anti-S6 Ribosomal Protein (Cell Signaling

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(1)

6/14/2016Cell Signaling LC3B Antibody Review Tamanna HossinWe used this antibody to look at autophagy occurrence and inhibition in mouse ES cells after different treatments.
(1)

2/26/2014LC3B Antibody for Tissue Staining Sandhya SandujaI have tried several antibodies to stain xenograft tisse for LC3B- a marker for autophagososmes. The cell signaling LC3B D11 antibody works great with almost no background, very specific staining. It ... Read Review

LC3B (D11) XP ® Rabbit mAb

LC3B (D11) XP ® Rabbit mAb from Cell Signaling Technology

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LC3B (D11) XP ® Rabbit mAb from Cell Signaling Technology

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