Roche
Anti HA High Affinity
11867423001
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HA tag is the most usual method to tag proteins.
Good HA antibody is important to detect protein expression and localization in-vitro after transfection of the protein of interest.
Immunofluorescence
MDCK cells Tribbles HA transfected.
1/20
10%HS 1%BSA in TBS
Anti rat FITC (1/500)
Not needed
FITC
Nuclear and cytosolic HA tag is detected in transient transfection.
None
Specificity.
Low dilution (1/20)
Good detection, with any non-specific staining in non-transfected cells