Cellometer Auto T4 Cell Counter from Nexcelom

February 07, 2013

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Ease-of-Optimization

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Stanford University
Structural Biology
Post-Doc
Cell count of human CD4+ cells after column isolation. Viable cells are circled green and non-viable cells are red.

Company:

REVVITY HEALTH SCIENCES, INC.

Product Name:

Cellometer Auto T4

Catalog Number:

CA-T4-MC

Image

We utilize a number of different tissue culture models including epithelial cells (lung and GI) and suspension cells (leukocytes), both primary cells and cell lines. We measure gene expression changes and normalize our data to cell number, so it is important to have accurate cell counts. In addition, we wanted an instrument that would allow us to exclude non-viable cells or cellular debris. We also needed an instrument that would accurately count a wide variety of cell types.

Experimental Design and Results Summary

Tips

If only one chamber of the slide is used, it can be wrapped in a kimwipe and saved for future use. The cell counts do not reflect the dilution performed when staining with Trypan blue, so the user needs to remember to multiply the counts obtained in this case by two.

Image Gallery

Cell count of human CD4+ cells after column isolation. Viable cells are circled green and non-viable cells are red.

Summary

The Good

Accurate counts, ability to count clumped cells and exclude background.

The Bad

Price. In addition to the instrument, slides can be a little on the pricey side.

The Bottom Line

We are happy with our purchase. The instrument has met our expectations.

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