Nucleic acid electrophoresis is the primary and most reliable molecular biology method for deciphering the content of DNA or RNA samples by size separation. The best practice used to tackle this method is to build and work off of a reliable system, which includes equipment, reagents, and visualization tools. Nucleic acid electrophoresis is optimized by type and percentage of agarose, type of buffer, and the type of instrument system used. One can choose a horizontal electrophoresis system for general size and content determination or a capillary electrophoresis instrument if genetic information is desired. Numerous easy to prepare size ladders, also called markers or standards, are available to measure the size of DNA or RNA fragments. The detection and analysis of isolated nucleic acid products by specific stains, such as UV activated Ethidium Bromide or fluorescent SYBRGreen®, is combined with gel documentation and imaging systems. Several gel purification kits are employed to obtain the highest quality and specific fragment of electrophoresed nucleic acid.
Features to consider when choosing a gel doc for your lab
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Set up a live demo with a Bio-Rad Imaging Specialist - Request a DemoUse the ChemiDoc MP Imaging System for flexible, high-sensitivity multiplex fluorescent and chemiluminescence western blot detection, imaging gels, analysis and documentation. This ...
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The Sapphire FL Biomolecular Imager is a field-upgradable laser scanner, with a wide selection of user-adjustable lasers and filters. It features 5-micron resolution, adjustable Z-plane focus and a wide field of view of 25cm X 25cm. Applications ...
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The background of this research is to evaluate nanoparticle and siRNA binding ability. We used GelRed nucleic acid stain as precast in agarose gels. The band is very clear under a UV transilluminator and the EtBr filter.
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We have been using this Odyssey® CLx Imaging System for 3 years for Western blot imaging. You can use any primary antibody, however, the secondary antibody is specific to LI-COR, it is conjugated to a fluorophore in order to image.
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