HPLC is a separation technique in which liquid is used to force the sample at high pressure through a column packed with stationary phase in order to identify and/or quantify the components. A typical HPLC system consists of a pump (consistent flow rate is key), an injector or autosampler, and at least one detector. Also, HPLC systems often use a column oven to adjust and control the temperature of the separation step. A variety of detector types are available, including UV-VIS, refractive index (RI), photo diode array, and fluorescence.
Biochemistry, biology, and the pharmaceutical industry all make use of the high resolution and fast separation possible with an HPLC system. Buyers can choose all-in-one systems, which tend to be more economical, or modular components, which ensure easier upgradeability in the future. Systems designed specifically for biomolecule separation are one area of interest. Ultra high-performance liquid chromatography, or UHPLC, is a common buzzword; the switch often requires additional method development, but can pay off in terms of throughput and speed.
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Shimadzu
- HPLC System
- Inquire
- 0.0001 – 5.0000 mL/min (1.0 – 44 MPa); 5.0001 – 10.0000 mL/min (1.0 – 22 MPa)
- 44 Mpa
Shimadzu
- HPLC / UHPLC
- 6 Columns
- 0.0001 to 10 mL/min
- 50 Mpa or 70 Mpa
Shimadzu
- Cannabis Analyzer
- 16 to 18 samples per 8 hr day (depends on method)
- 1.6 mL/min
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Shimadzu
- Cannabis Analyzer
- 16 to 18 samples per 8 hr day (depends on method)
- Inquire
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Shimadzu
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- 0.0001 to 5 mL/min
- 66 MPa
Shimadzu
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- Inquire
- 0.0001 to 10 mL/min
- 66 MPa
Shimadzu
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Shimadzu
- Preparative Supercritical Fluid Chromatography
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- 10 to 150 mL/minute
- Inquire
Bio-Rad
- Preparative Liquid Chromatography System
- Depends on model
- 0.001–10 mL/minute (F10 pump) / 0.01-100 mL/min (F100)
- 3650 psi / 1450 psi
Bio-Rad
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- Inquire
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Bio-Rad
- Preparative/Analytical HPLC System
- Up to 5 columns
- 0.001–10 mL/minute
- 3,650 psi
Bio-Rad
- Preparative Liquid Chromatography System
- Up to 5 columns
- 0.001 - 10 mL/min
- 3,650 psi (252 bar, 25.2 MPa)
Bio-Rad
- Preparative Liquid Chromatography System
- Up to 10 columns (with two 5 column pumps)
- 0.01 - 100 mL/min
- 1,450 psi (100 bar, 10 Mpa)
Bio-Rad
- Preparative Liquid Chromatography System
- Up to 5 Columns
- 0.01 - 100 mL/min
- 1,450 psi (100 bar, 10 Mpa)
Bio-Rad
- Preparative Liquid Chromatography System
- Up to 5 columns
- 0.01 - 100 mL/min
- 1,450 psi (100 bar, 10 Mpa)
Bio-Rad
- Preparative Liquid Chromatography System
- Up to 10 columns (with two 5 column pumps)
- 0.01 - 100 mL/min
- 1,450 psi (100 bar, 10 Mpa)
SCIEX
- High performance liquid chromatography–mass spectrometry
- 6 micro titer plates (96 or 384 well);
6 vial adapt
- 1 to 10 uL/min, 5 to 50 ul/min, or 20 to 200 ul/min (depending on model)
- 10,000 PSI
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SCIEX
- High-flow LC
- Up to 8 x 12.5 cm columns
- EXIONLC 2.0 system:12,500 psi (860 bar) ; EXIONLC 2.0+ system: 18,000 psi (1240 bar)
Cytiva Life Sciences
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- Inquire
- 0.001 mL/min to 25 mL/min
- 10 MPa (100 bar, 1450 psi)
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