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Title
Quantifying the Terminal Complement Complex (C5b-9) in Plasma
September 08, 2014
Activation of any of the three complement pathways (classical, alternative, or lectin) results in the formation of the terminal complement complex, ...
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3D Made EZ: Tools for 3D Cell Culture
September 08, 2014
The greater physiological relevance of 3D cultures can result in different cell behaviors, and even experimental outcomes, than 2D cultures. Yet as ...
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PPi Release Assay Works Well
September 08, 2014
I used the Lonza PPi release assay to measure the activity of a pyrophosphorylase. I also compared the activity to a suspect pyrophosphorylase, ...
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Western Blotting - CLK2 Antibody in Human Endothelial Cells
Aviva Systems Biology
September 05, 2014
Clk2 protein expression was characterized via western blot using the CLK2 Antibody (1:1000).
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Detecting TGFß1 in Human Sarcoma
Santa Cruz Biotechnology, Inc.
September 05, 2014
The TGF family of proteins are implicated in many tumor processes, such as development, growth, migration, and metastasis. A good antibody for TGF ...
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Are your T cells activated?
BioLegend
September 05, 2014
The stimulation of T cells is carried out by different methods. In order to check if your stimulation worked or not, there are many factors you can ...
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Muscle Actin Identification by IHC
September 05, 2014
We use this antibody for some tumor types classifications. It can recognizes specific Actin present in skeletal muscle, not in smooth muscle. We use ...
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Quantify Epigenetic Mods with These DNA Methylation Tools
September 03, 2014
It’s generally impractical to apply genome-wide scans to large numbers of samples, in part for economic reasons but also because there’s no reason to ...
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Norgen Urine Total RNA Purification Kit
Norgen Biotek Corporation
September 02, 2014
I am interested in the expression of genes in the urine of transplant, in order to identify biomarkers for a disease called graft versus host disease....
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Determination of the IgG Subclass of a Human Autoantibody
Abcam
September 02, 2014
Our laboratory is interested in an autoantibody that we identified several years ago in cancer patients. The presence of this autoantibody correlates ...
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Great anti-FLAG Monoclonal Antibody
September 02, 2014
A FLAG tagged construct was overexpressed in HEK293 cells and WB was utilized to track the expression level of the protein.
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PCR Purification via MinElute PCR Purification Kit
September 01, 2014
We study the various mechanisms of gene silencing responsible for disease resistance in poultry. We specifically study various target genes through ...
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DNA and Protein Visualization
ProteinSimple, a Bio-Techne Brand
September 01, 2014
This equipment was installed as shared facility 3 years back at Ohio Sate University in building named Tzagournis. Our lab work involves running DNA ...
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Cell Viability Tools for Flow Cytometry
August 29, 2014
A basic but crucial task facing researchers who analyze live-cell populations is to assess their viability. In its simplest form, this involves ...
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You Can Count (Cells) On These Coulter-Based Cell Counters
August 26, 2014
Coulter principle-based cell counters can reveal both particle number and size, and some have capabilities to yield additional data. Here is what you ...
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Edit Your Way to Better DNA with These CRISPR/Cas Tools
August 21, 2014
The CRISPR/Cas system has been put to work in a flurry of publications across species from Anopheles to zebrafish. Naturally, molecular-tool ...
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Detection of LAP+ Treg Cells by Flow Cytometry
Mitesh Dwivedi
The aim of the experiment was to detect LAP+ CD4+CD25+ regulatory T cells (Tregs) isolated from whole blood of humans.
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