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Date Published
Title
Next-Gen DNA Sequencing: Fall 2014 Update
November 12, 2014
Sequencing instrumentation hasn’t changed since we looked at the market in February 2014, but that’s not to say all is as it was. New sample-...
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Cleaved caspase-3 Antibody for WB
Cell Signaling Technology
November 12, 2014
Programed cell death (PCD) is a critical process that underlies normal tissue development and serves as a homeostatic mechanism to maintain cell ...
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Great Antibody for FLAG immunoprecipitations
November 11, 2014
Our lab uses Anti-FLAG® M2 Magnetic Beads (produced in mouse, Sigma) for protein interaction co-immunoprecipitations. Invariably, some of the mouse ...
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Clear and Crisp DnaK Antibody
Abcam
November 11, 2014
We generally use DnaK protein levels as a cytosolic control for western blotting. This allows us to determine if general protein levels are expressed ...
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Western blotting via anti-Bcl-x
November 10, 2014
Anti-Bcl-x protein expression was characterized via western blot by using purified rabbit Anti-Bcl-x (1:500)
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MCP-1 detection in A549 cells
Santa Cruz Biotechnology, Inc.
November 10, 2014
MCP-1 protein expression was characterized via Western blot by using purified MCP-1 (C-17) Antibody
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FFPE or Frozen? Working with Human Clinical Samples
November 07, 2014
Peering into human tissues—to study morphology, cell biology, biochemistry and more—is made possible using sections of biopsied tissue that have been ...
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GAPDH protein expression analysis via Western blotting
MilliporeSigma
November 07, 2014
GAPDH protein expression was characterized via Western blot using the anti-GAPDH antibody (1:10000).
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Western blot via p44/42 MAPK (Erk1/2) Mouse mAb from Cell Signaling Technology
Cell Signaling Technology
November 07, 2014
Erk1/2 protein expression was characterized via Western blot in HL-1 cells
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Excellent Ab for intracellular staining of mouse IFN-g
BioLegend
November 06, 2014
We are looking at the intracellular staining of mouse IFN-gamma in CD4 memory T cells when stimulated by PMA, Ionomycin and BFA.
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CD3 Antibody works at high concentration
BioLegend
November 06, 2014
We are trying to sort CD3 population in mouse spleen cells by Brilliant Violet 650™ anti-mouse CD3 Antibody.
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SuperBlock Really is Super
November 05, 2014
I have been working with SuperBlock as a blocking buffer for the last three years and it has dramatically improved the quality of my western blots and...
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QIAxcel for Saving Time
QIAGEN - Sample to Insight
November 05, 2014
Our lab studies a specific subset of B cells. Our research focuses on determining the overall function of these B cells in both humans and mice. We ...
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Nature's Vectors: Picking the Right Viral Expression System
November 04, 2014
Viruses have evolved along with just about every species studied, and they have developed crafty ways of insinuating themselves into cells and turning...
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A great DNA ladder
November 04, 2014
We run a lot of 1-2% agarose gels to test the size of our sample DNA, especially PCR products. We have used several types of ladders in the past, but ...
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Primer is superier to other version of ECL
November 04, 2014
I was trying to get a Western blot done for tissue expression of the trans gene from transgenic mice we created. I was having trouble with the ECL ...
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Detection of LAP+ Treg Cells by Flow Cytometry
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The aim of the experiment was to detect LAP+ CD4+CD25+ regulatory T cells (Tregs) isolated from whole blood of humans.
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