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Date Published
Title
Human T-Cell Isolation
May 12, 2016
The kit provided by STEMCELL allows for facilitated isolation of human lymphocytes from peripheral blood mononuclear cells. It involves a magnetic ...
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Anti-Human CD8 Microbeads - A Consistent and Reliable Method to Positively Select CD8+ T Cells from PBMC
Miltenyi Biotec
May 12, 2016
Our lab routinely works with purified immune cell subsets from human peripheral blood mononuclear cells (PBMC). In order to obtain highly pure CD8+ T ...
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Thermo p21 Antibody (701151) Never Works
Thermo Fisher Scientific
May 12, 2016
I tested p21 expression level in Huh7 cells. After treating doxorubicin, p21 is up-regulated, which is confirmed by RT-qPCR. Compared to the p21 ...
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Good Brachyury Antibody for Immunofluorescence in Human Cells
R&D Systems, a Bio-Techne Brand
May 12, 2016
The aim of this experiment was to detect mesodermal cells derived from human iPS cells.
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Great Cytotoxic T-Cell Marker
Tonbo Biosciences
May 12, 2016
Flow cytometry was used to probe epithelial dynamics and the immune infiltrates in human prostate tissues. Epithelia was investigated with CD45-CD325+...
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A Reliable Antibody for Total Akt Protein
Cell Signaling Technology
May 11, 2016
We usually use 1:1000 dilution for total akt expression level in tissue or cell lines. This secondary antibody dilution can be used from 1:5000 to 1:...
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Easy Storage of RNA
May 11, 2016
RNA extraction can be tedious and requires caution. Sometimes we need to do RNA extraction weeks later and that is when the stability of RNA becomes a...
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A Solution for Isolation of Magnetized Cells
Miltenyi Biotec
May 11, 2016
We used this kit for isolation of magnetized cells; we were able to isolate magnetized cells from a cell culture as can be observed in the figure.
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Flow Cytometric Analysis of CD86
BioLegend
May 11, 2016
We used this antibody to monitor activation of myeloid / APC cells during M.tb infection in mice. Works well and detects a clean population of ...
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Great T-Cell Marker
Tonbo Biosciences
May 11, 2016
Flow cytometry was used to probe epithelial dynamics and the immune infiltrates in human prostate tissues. Epithelia was investigated with CD45-CD325+...
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Non-invasive small animal imaging using smart activity based probes (iABP).
Vergent Bioscience
May 11, 2016
Small animal imaging enables researchers to examine below the surface and understand cellular processes in a relevant biological environment. While in...
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ELISA for IL-6
Novus Biologicals, a Bio-Techne Brand
May 11, 2016
My project is related to sepsis and some possible inhibitors of sepsis related receptors. We wanted to check how my inhibitors are effective, so as an...
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In Situ Proximity Ligation Assay (PLA) Kit components: A Comprehensive Review
May 10, 2016
The aim of my research was to detect if 14-3-3? protein interacts with a-tubulin at the meiotic spindle of eggs during mouse oocyte maturation. The ...
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Compact Basic Sonicator for Cell Disruption
Qsonica, LLC
May 10, 2016
Some of our experimental protocols call for physical disruption of cells. This can be accomplished by sonication. This compact unit is ideal to lyse ...
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Secondary Antibody for Immunohistochemistry Staining
MilliporeSigma
May 10, 2016
The dilution used for this is 1:500 or 1:400, it is diluted in PBS and incubated for 30 minutes to 1 hour. This is a very reliable secondary antibody ...
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Great Leukocyte Marker
Tonbo Biosciences
May 10, 2016
Flow cytometry was used to probe epithelial dynamics and the immune infiltrates in human prostate tissues. Epithelia was investigated with CD45-CD325+...
Watch Video
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Excellent Monoclonal CD3 Marker for Dogs and Cats
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