ÄKTA avant™ 25 from Cytiva

Product Specs
ItemÄKTA avant™ 25
CompanyCytiva Life Sciences
Price Get Pricing Get Quote for this Product
Catalog Number28930842
Dimensions(WxDxH) 860 x 710 x 660 mm
QuantityEA
ApplicationsAffinity chromatography, gel filtration/size exclusion chromatography, ion exchange chromatography, and hydrophobic interaction chromatography
Wavelength(s)190 to 700 nm
Electrical Requirements100–120/220–240 VAC; 800 VA
Flow Rate0.001 mL/min to 25 mL/min
Maximum Pressure10 MPa (100 bar, 1450 psi)

Cytiva Life Sciences

(1) Chelator Conjugation Chemistry Matters: Squaramide Linker Enhances Tumor Contrast in 68 Ga-labeled Anti-CEA Nanobody TracerACS OmegaApril 7, 2026Jelena Saliën, Sam Floru, Michiel Van Hove, Tessa De Pauw, Romane Vizier, Michael Claron, Jessica Bridoux, Victor Goncalves, Franck Denat, Sophie Hernotbioconjugation function (see the SI). Afterward, excess chelator was removed using SEC on a ÄKTA pure M or ÄKTA avant 25 protein purification system (Cytiva, Hoegaarden, Belgium) equipped with a Superdex 30 Increase 10/300

(2) AI-guided prefusion stabilization of the human coronavirus OC43 spike protein enables universal embecovirus antigen designPLoS PathogMarch 23, 2026Jelle M. Melchers, Jarek Juraszek, Ruben J.G. Hulswit, Daan van Overveld, Lam Le, Frank J.M. van Kuppeveld, Daniel L. Hurdiss, Berend-Jan Bosch, Johannes P.M. Langedijk, Mark J.G. Bakkerspurified from culture supernatants through a two-step Galantus nivalis lectin-based protocol on an ÄKTA Avant 25 system (GE Healthcare). Clarified supernatant was applied to a Tricorn 10/50 column (Cytiva) packed

(3) The Multi-Attribute Method (MAM), An Advanced LC-MS Approach for Protein A Resin Performance and Lifecycle EvaluationAntibodies (Basel)March 23, 2026Jingming Zhang, Matthew Larsen, Timothy Blanc, Babita S. Parekh, Ming-Ching Hsiehbottle-top filter prior to loading onto the Protein A column. The chromatography was performed on an ÄKTA Avant 25 system (Cytiva, Marlborough, MA, USA) at ambient temperature, controlled by UNICORN software (v.7.6)

(4) Integrative structural insights into the IgG-FcRn interactions revealed by engineered FcRn-immobilized affinity chromatographyCommunications BiologyMarch 3, 2026Masato Kiyoshi, Takuo Suzuki, Naruaki Inoue, Ryoko Otake, Tatsuya Yumoto, Linko Hirono, Yosuke Terao, Hiroko Shibata, Teruhiko Ide, Satoru Nagatoishi, et al.non-two-state model. FcRn affinity chromatography The FcRn affinity chromatography was performed using an ÄKTA Avant 25 (Cytiva, Sweden). Mobile phase A was 50 mM 2-morpholinoethanesulfonic acid (MES), 150 mM NaCl (pH 6.5),

(5) Structural and stability differences among GI norovirus virus-like particles produced in silkworm–baculovirus expression vector systemFrontiers in MicrobiologyJanuary 8, 2026Yuto Tsurumi, Akitsu Masuda, Jian Xu, Hiroaki Mon, Takahiro Kusakabe, Jae Man Leestirring. 2.4.2 Anion exchange chromatography Anion exchange chromatography was performed using an ÄKTA avant 25 system (Cytiva, Tokyo, Japan). Samples were applied either to a 1 mL CIMmultus™ QA columns (Sartorius,

(6) Perturbing H-NS function reveals roles in restricting virulence heterogeneity and pathogen adaptationbioRxivJanuary 6, 2026Leah McLelland, Dakshya Karki, Madison Spratt, Miracle Burt, Jonathan Zhao, Keara LanepH 8.0, 300 mM NaCl, 5% glycerol, 5 mM β-mercaptoethanol) and run at a flow rate of 0.8 mL/min on an ÄKTA Avant 25 system (GE Healthcare) at Northwestern University’s Biofoundry Core. Peak fractions were analyzed by

(7) H3K27me3 ‐Mediated Epigenetic Silencing of FgHMG1 Enables Fungal Host Immune EvasionPlant Biotechnology JournalJanuary 6, 2026Xiaozhen Zhao, Bingqin Yuan, Peixue Ma, Yajie Cai, Yan Huang, Yaxuan Wang, Zhen Cheng, Yuan Chen, Minghong Zheng, Ran Zhang, et al.obtained through centrifugation (10 000 × g, 4°C, 20 min) and subjected to affinity purification using an ÄKTA avant 25 system (GE Healthcare). Protein expression profiles were verified by sodium dodecyl sulphate polyacrylamide gel electrophoresis

(8) A Longitudinal 3D Live-Cell Imaging Platform to Uncover AAV Vector–Host Dynamics at Single-Cell ResolutionInternational Journal of Molecular SciencesDecember 25, 2025Marlies Leysen, Nicolas Peredo, Benjamin Pavie, Benjamien Moeyaert, Els Henckaerts#514-0334). rAAV purification was performed on an ÄKTA Pure protein chromatography system (Cytiva, Äkta Avant 25) using a HiTrap Capto AVB column (Cytiva, Uppsala, Sweden, #17372212). An iodixanol step gradient was

(9) Establishment of a novel human amniotic epithelial-derived cell line, HAT, for high-yield AAV vector productionMolecular Therapy Methods & Clinical DevelopmentDecember 11, 2025Yugo Hirai, Yu-Hsin Chang, Arisa Yamamoto, Ryo Asahina, Rena Moromizato, Mawo Kinoshita, Kazuko Aizawa, Manami Miyai, Michi Kubota, Takayuki Horiuchi, et al.affinity capture chromatography using a POROS GoPure AAVX pre-packed column (Thermo Scientific) on an Äkta avant 25 system (GE Healthcare Life Sciences). The affinity purification column was equilibrated with five column

(10) Tailored collagen binding of albumin-fused hyperactive coagulation factor IX dictates in vivo distribution and functional propertiesNature communicationsSeptember 29, 2025Aaen KH, Testa MF, Nilsen J, Tarantino R, Canepari C, Benedusi M, Benjakul S, Nyquist-Andersen M, Herigstad ML, Cantore A, et al.was performed by using a Superdex 200 Increase 10/300 GL column (Cytiva, #28-9909-44) coupled to an ÄKTA avant 25 instrument (Cytiva) using Unicorn Software (Cytiva), with PBS as running buffer. The monomeric protein

(11) Calreticulin in the nasal mucus promotes neutrophil migration and pathogen clearanceCell Commun SignalAugust 26, 2025Yichao Ma, Xinming Qin, Shiqi Liu, Ruoyang Lin, Baoyan Meng, Jian Lin, Qian Yangsubsequent analyses [27]. This extracted protein was loaded onto a Superdex-200 column connected to an ÄKTA Avant 25 instrument (GE Healthcare, Chicago, USA). Protein complexes were separated by passing PBS at 1.0 mL/min.

(12) Enhanced plasma half-life and efficacy of engineered human albumin-fused GLP-1 despite enzymatic cleavage of its C-terminal endCommun BiolMay 26, 2025Jeannette Nilsen, Kristin Hovden Aaen, Sopisa Benjakul, Fulgencio Ruso-Julve, Thomas Uwe Greiner, Daniela Bejan, Maria Stensland, Sachin Singh, Tilman Schlothauer, Inger Sandlie, et al.with a 30 kDa cutoff by centrifugation at (290–400) × g at 4 °C. Subsequently, SEC was conducted on an ÄKTA avant 25 instrument (Cytiva) coupled to a Superdex 200 Increase 10/300 GL Column (Cytiva). Isolated monomeric

(13) Monomers, Dimers, and Oligomers of Pyroglutamate-Modified α-Synuclein Fragments Exhibit Distinct Biophysical CharacteristicsACS Chem NeurosciMay 21, 2025Alexandra Bluhm, Wei Xiang, Frank Wien, Aurelien Thureau, Maelenn Chevreuil, Bertrand Raynal, Stefanie Geissler, Michael Wermann, Stephan Schilling, Philippe Bénas, et al.subsequently loaded at a flow rate of 0.8 mL/min onto a Superdex 75 Increase 10/300 GL column on an ÄKTA avant 25 system (Cytiva, Freiburg, Germany). The eluted peaks were monitored at 220 and 280 nm. SH-SY5Y cells

(14) SNAP-Tag-Based Recombinant Photoimmunotherapeutic Agents for the Selective Detection and Killing of Light-Accessible Melanotransferrin-Expressing Melanoma and Triple-Negative Breast CancerCancer MedMay 6, 2025Suzanne Hippolite Magagoum, Fleury Augustin Nsole Biteghe, Gael Tchokomeni Siwe, Dirk Lang, Nkhasi Lekena, Stefan Barthprocess using a 5 mL Ni2+ Sepharose affinity column (His‐Trap Excel, GE Healthcare, USA) mounted on an ÄKTA Avant 25 system (GE Healthcare, Germany), as previously reported [10, 54]. 2.3 Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis

(15) Synergistic Insecticidal Activity Against Hyphantria cunea by Cry9Aa3 Mutants and Cry1Ah CombinationsInt J Mol SciApril 8, 2025Pengdan Xu, Zeyu Wang, Ying Zhang, Jiaxing Han, Changlong Shu, Min Liao, Jie Zhang, Lili GengProteins were purified by nickel-affinity chromatography [36], and the protein purification system AKTA (ÄKTA™ avant 25, Cytiva, Wilmington, NC, USA) was used with a desalting column (HiPrep™ 26/10 Desalting, Cytiva, USA)

(16) Enhanced Hemodynamics of Anisometric TPMS Topology Reduce Blood Clotting in 3D Printed Blood ContactorsAdvanced Healthcare MaterialsJanuary 1, 2025Lukas T. Hirschwald, Franziska Hagemann, Maik Biermann, Paul Hanßen, Patrick Hoffmann, Tim Höhs, Florian Neuhaus, Maerthe Theresa Tillmann, Petar Peric, Maximilian Wattenberg, et al.GmbH, Germany). After complete curing of the adhesive, the module was installed in an HPLC system (Äkta avant 25 with Unicorn 6.1, GE Healthcare Bio‐Science AB, Sweden) in place of a column. First, the module was

(17) An influenza mRNA vaccine protects ferrets from lethal infection with highly pathogenic avian influenza A(H5N1) virusScience Translational MedicineDecember 18, 2024Masato Hatta, Yasuko Hatta, Angela Choi, Jaber Hossain, Chenchen Feng, Matthew W. Keller, Jana M. Ritter, Ying Huang, Emma Fang, Elizabeth A. Pusch, et al.protein was purified using a Ni2+ HisTrap FF 5-ml column (Cytiva) on fast protein liquid chromatography (ÄKTA avant 25, Cytiva), followed by size exclusion chromatography on a Superose 6 Increase column (Cytiva) with a

(18) A foldon-free prefusion F trimer vaccine for respiratory syncytial virus to reduce off-target immune responsesNature MicrobiologyNovember 20, 2024Mark J. G. Bakkers, Freek Cox, Annemart Koornneef, Xiaodi Yu, Daan van Overveld, Lam Le, Ward van den Hoogen, Joost Vaneman, Anne Thoma, Richard Voorzaat, et al.a 0.22 µm PES vacuum filter (Nalgene). RSV F trimers were purified using a two-step protocol on an ÄKTA Avant 25 system (Cytiva). Clarified supernatant was diluted with 1.33 volume of Milli-Q water and conditioned

(19) Engineering a cleaved, prefusion-stabilized influenza B virus hemagglutinin by identification and locking of all six pH switchesPNAS NexusOctober 11, 2024Jarek Juraszek, Fin J Milder, Xiaodi Yu, Sven Blokland, Daan van Overveld, Pravien Abeywickrema, Sem Tamara, Sujata Sharma, Lucy Rutten, Mark J G Bakkers, et al.proteins From the harvested culture supernatants, HA proteins were purified by a two-step protocol using and ÄKTA Avant 25 system (GE Healthcare Life Sciences). Clarified supernatant was loaded on a C-tag XL 5 mL prepacked

(20) Engineering of pH-dependent antigen binding properties for toxin-targeting IgG1 antibodies using light-chain shufflingStructureSeptember 5, 2024Tulika Tulika, Fulgencio Ruso-Julve, Shirin Ahmadi, Anne Ljungars, Esperanza Rivera-de-Torre, Jack Wade, Monica L. Fernández-Quintero, Timothy P. Jenkins, Selma B. Belfakir, Georgina M.S. Ross, et al.size-exclusion chromatography Analytical SEC was performed using a Superdex 200 increase 10/300 GL column (Cytiva) coupled to an ÄKTA avant 25 (Cytiva). Injections were performed in 1× PBS (Gibco) and 10 μg of the individual antibodies was injected

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ÄKTA avant™ 25

ÄKTA avant™ 25 from Cytiva

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Cytiva Life Sciences

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ÄKTA avant™ 25 from Cytiva

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