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Caspase-Glo 3/7 Assay
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Caspase-Glo 3/7 Assay
Caspase-Glo 3/7 Assay
Caspase-Glo 3/7 Assay from Promega
(1)
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Citations:
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Figures:
(4)
Supplier Page
Supplier Page from
Promega for
Caspase-Glo 3/7 Assay
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Description
Caspase-Glo 3/7 Assay is a homogeneous luminescent assay that measures caspase-3/7 activities
Product Specs
Company Info
Citations
Reviews
Product Reviews
Product Specs
Item
Caspase-Glo 3/7 Assay
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
G8091
Detection Target
Caspase 3 / Caspase 7
Method
Luminescent
Quantity
10ml
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (1042)
(1)
A versatile VLP-mediated CRISPR-RNP platform for precise genome editing and durable epigenome silencing in cancer
Molecular Therapy Nucleic Acids
September 8, 2026
Sungjin Ju, Jang Hyeon Lee, Jiyun Yang, Tae Yeong Jeong, Chang Geun Choi, Yerim Lee, Seongkwang Cha, Jeongeun Park, Sol Pin Kim, Sanghun Kim,
et al.
v.10.7. Caspase-3/7 activity
assay
Caspase-3/7 activity was measured using the
Caspase-Glo 3/7 assay
(
Promega
,
G8091
) according to the manufacturer’s instructions. Cells were seeded in 96-well plates at 5 × 103 cells
(2)
Potent Anti-adipogenic Activity of Cyclopeptide Mallotumide A on Murine 3T3-L1 Adipocytes
ACS Omega
July 28, 2026
Chayanee Laowittawat, Phanthira Boonprasert, Natthapat Sawektreeratana, Sakchai Hongthong, Vichai Reutrakul, Chutima Kuhakarn, Sarawut Jitrapakdee
differentiation. Following treatment, caspase-3/7 activity was determined using the
Caspase-Glo 3/7 assay
(
G8091
,
Promega
, Madison, WI, USA). 100 μL of Caspase-Glo 3/7 reagent was added directly to each well, and
(3)
Synthetic Toll-Like Receptors for Control of Innate Immunity With Far-Red Light
Adv Sci (Weinh)
July 27, 2026
Anna V. Leopold, Vladislav V. Verkhusha
wells served as positive controls.
After treatment
, 100 µL of Caspase‐Glo 3/7 reagent (
Promega
, Cat. #
G8090
) equilibrated to room temperature was added directly to each well. Plates were shaken for 30 s at 300
(4)
DNAJB12/14 redox switching directs chaperone- and Bax/Bak-dependent ER protein reflux
Redox Biol
July 25, 2026
Laila Abu Madegam, Noa Gavriel, Raifu Tolulope Adebisi, Aeid Igbaria
with membranous
endoplasmic reticulum
. Caspase-3/7 activity: The
Caspase-Glo 3/7 assay
kit (
Promega
#
G8090
) was used following the manufacturer's protocol. Briefly, 100 μL of Caspase-Glo 3/7 reagent was added
(5)
Distinct roles of COPI proteins attenuated in cell senescence
Sci Adv
July 24, 2026
Krystyna Mazan-Mamczarz, Eleanor J. Wind, Apala Pal, Martin Salamini-Montemurri, Dimitrios Tsitsipatis, Kyoung Mi Kim, Rachel Munk, Jixiang Leng, Chang Hoon Shin, Jennifer L. Martindale,
et al.
measured in a 96-well solid white-bottom plate following the manufacturer’s instructions (
Promega
,
G8091
). The caspase 3/7 substrate, dissolved in the provided buffer solution, was added to the media of each
(6)
A Microgel-Based Platform for Tunable Expansion and Function of γδ T-cells
bioRxiv
July 24, 2026
Favour Omafuvwe Obuseh, Junzhe Lou, Michelle Chang, Anqi Chen, David Weitz, David Mooney
then allowed to reach room temperature before addition of Promega caspase 3/7
assay
reagent(
Promega
,
G8091
) as described by the manufacturer. The mixture was allowed to react for 1 hour then a
BioTek
Synergy H1
(7)
miR-145-5p Is Required for the Antitumor Activity of Strophanthus gratus-Derived Ouabain in Colorectal and Breast Cancer
Pharmaceuticals (Basel)
July 17, 2026
Jianxiong Xu, Zhiming Lv, Zenan Xu, Han Zhang, Mingyu Xia, Wenfang Li
containing the lyophilized Caspase-Glo® 3/7 Substrate (
Promega Corporation
, Madison, WI, USA, Cat. No.
G8091
), followed by swirling until the substrate was completely dissolved. Then, 100 μL of the reconstituted
(8)
Suppression of ATM kinase signaling accelerates cellular senescence
Stem Cell Reports
July 14, 2026
Kei-ichi Ishikawa, Takahiro Shiga, Takumi Hirose, Naoko Kuzumaki, Sakura Miyoshi, Akihiro Yamaguchi, Hidetaka Tamune, Avijite Kumer Sarkar, Kento Nakai, Kazuyoshi Baba,
et al.
V-FITC Apoptosis Detection Kit,Nacalai Tesque,Cat#15342-54
Caspase-Glo 3/7 Assay
System,
Promega
,Cat#
G8090
CellTiter-Blue Cell Viability Assay
,
Promega
,Cat#
G8080
CellTiter-Glo 2.0
Cell Viability Assay
,
Promega
,Cat#
G9241
Human
(9)
iPLA2β-null mice exposed to natural pathogens exhibit hepatocellular fibrotic injury with male-biased alteration of glycerolipid metabolism
Front Immunol
July 8, 2026
Weihong Xu, Jiliang Wang, Gang Li, Gerhard Liebisch, Walee Chamulitrat
(Krefeld, Germany). Plasma caspase3/7 activities were determined using
Caspase 3
/7Glo
assay
kits (#
G8090
,
Promega
, Mannheim, Germany), and
luminescence
was measured with a Fluostars Optima (BMG Labtech GmbH,
(10)
The MIRO1-BAX Complex Dictates Life and Death at the Mitochondrial Gate
bioRxiv
July 1, 2026
Alva G. Sainz, Chulhwan S. Kwak, Kwang Bog Cho, Sejal A. Sripadanna, Brandon H. Bergsneider, Zoe Zizzo, Aarooran S. Durairaj, Zehui Du, Ian Cooney, Anthony Venida,
et al.
with 50 μL of fresh medium following cell treatments. Then, 50 μL of complete Caspase-Glo reagent (
G8090
,
Promega
) was added to each well. Plates were incubated at room temperature for 1 hour before
luminescence
(11)
Recurrent patterns of TOP1-mediated neuronal genomic damage shared by major neurodegenerative disorders
Cell
July 1, 2026
Zinan Zinan Zhou, Lovelace J. Luquette, Guanlan Dong, Junho Kim, Jayoung Ku, Kisong Kim, Nandini Ramesh, Mingyun Bae, Ann Caplin, Diane D. Shao,
et al.
commercial
assays
EasyTag EXPRESS 35S
Protein Labeling
Kit,
PerkinElmer
,
NEG772014MC
CaspaseGlo 3/7,
Promega
,
G8090
TruSeq ChIP Sample Prep Kit,Illumina,IP-202-1012 Deposited data,Deposited data,Deposited data Raw
(12)
FATP2-mediated lipid metabolism enhances chimeric antigen receptor T-cell therapy resistance in B-cell acute lymphoblastic leukemia
Leukemia
June 30, 2026
Clarissa Garcia, Kaylyn Utley Lyons, Julian Grandvallet Contreras, Tian Liu, Alexis J. Donnelly, Amanda J. Novak, Amy Argabright, Colin Anderson, Abby Grier, Sabrina Smith,
et al.
subtracted. Caspase activity in CAR-T
co-cultures
was quantified using the
Caspase-Glo® 3/7 Assay
(
Promega
G8090
). An equal volume of reagent was added to each well, plates were incubated for 60 min, and
luminescence
(13)
VKORC1L1-mediated vitamin K recycling counters ferroptosis to promote endothelial repair
Sci Rep
June 19, 2026
Elena Repges, Adem Aksoy, Katrin J. Czogalla-Nitsche, Andreas Zietzer, Cornelius Müller, Johannes Oldenburg, Sebastian Zimmer, Georg Nickenig, Vedat Tiyerili, Muntadher Al Zaidi
was quantified (Ex 568 nm/Em 585 nm). Apoptosis was determined by the
Caspase-Glo® 3/7 assay
(
Promega
G8091
): the reagent was added for 2 h at room temperature before recording
luminescence
produced by luciferase.
(14)
Reverse engineering of BNIP3 identifies a mitochondrial protective peptide
Nat Commun
June 17, 2026
Ulrike B. Hendgen-Cotta, Anna Roth, Christine Beuck, Daniel Messiha, Stephan Settelmeier, Shah Bahrullah Shah, Sebastian Korste, Kenny Bravo-Rodriguez, Mike Blueggel, Feyza Cansiz,
et al.
µM STS, 26 and 52 µM B-017/negative control peptide, 4 h) using the Caspase-Glo® M3/7Assay (
Promega
,
G8090
), according to the manufacturer’s instructions using the BMG FLUOstar Omega Microplate Reader. Reactive
(15)
KLF6 activation marks an angiogenic and apoptosis resistant endothelial phenotype in pulmonary arterial hypertension
Commun Biol
June 15, 2026
Rehab Alharbi, Merve Keles, Nadia Fernandes, Hannah Maude, Adam Fellows, Richard D. Williams, Chien-Nien Chen, Nathalie Lambie, Nik Matthews, May Al-Sahaf,
et al.
measuring caspase 3/7 activity using a
Caspase-Glo 3/7 Assay
Kit (
Promega
, Southampton, UK, Cat. No.
G8091
), according to the manufacturer’s instructions.
TUNEL
apoptosis assay
TUNEL Assay
was performed using
(16)
Cannabigerol and Cannabichromene Induce Lung Cancer Cell Death and Apoptosis-Contribution of PPARα to Cannabigerol Effects
Antioxidants (Basel)
June 15, 2026
Theresa Spengler, Felix Wittig, Marcus Frank, Burkhard Hinz
durations.
Promega
luciferase
assays
were used to
measure
caspase activity (
Caspase-Glo® 3/7 assay
, #
G8091
;
Caspase-Glo® 8 assay
, #
G8201
;
Caspase-Glo® 9 assay
, #
G8211
). For this purpose, the corresponding Caspase-Glo®
(17)
Ionic regulation of cancer cell stiffness and metastatic colonization via the MRTFA-KCNMB1 axis
Developmental Cell
June 10, 2026
Alexa M. Gajda, Mohamed Haloul, Vinay Pai, Keyvan Mollaeian, Khushi J. Patel, Raymundo Rodríguez-López, Katie M. Beverley, Mark A. Sanborn, Kihak Lee, Caitlyn C. Castillo,
et al.
(
Molecular Devices
). Cell death was determined by caspase 3/7 cleavage using the
Caspase-Glo 3/7 Assay
(
Promega
#
G8091
) according to the manufacturer’s instructions 24 hours post-treatment with the
inhibitors
and by using
(18)
DNA replication stress and translational repression converge to drive CDK1- and caspase-dependent apoptosis in Ewing sarcoma
Oncogene
June 10, 2026
Stacia L. Koppenhafer, Mary V. Thomas, Mian T. Mhindu, Jessica A. O. Zimmerman, David J. Gordon
activation
assay
Caspase-3/7 activation was measured using the Caspase-Glo 3/7
Luminescence
assay
(
Promega
,
G8090
), according to the manufacturer’s instructions. Reverse phase protein array (RPPA) RPPA analysis of
(19)
Multi-omics and experimental evidence in human chondrocytes identify caspase-8 as a non-apoptotic regulator of inflammatory, senescent, and fibrotic signaling in osteoarthritis
Cell Commun Signal
June 6, 2026
Jian Mei, Penghui Wei, Nicole Schäfer, Marianne Ehrnsperger, Brian Johnstone, Eva Matalova, Susanne Grässel
using the Caspase-Glo®
luminescent assays
(
Promega
, Walldorf,
caspase-1
: Cat.
G9952
;
caspase-3
/7: Cat.
G8091
; caspase-8: Cat. #
G8201
and caspase-9: Cat,
G8211
).
Chondrocytes
(1 × 10⁴ cells per well) were seeded
(20)
Cyanine-modified ssODNs enhance CRISPR-Cas9 HDR in stem cell embryo models via chromatin and chemical modulation
Nature Communications
June 2, 2026
Baolei Yuan, Yeteng Tian, Wei-Bin Lin, Chongwei Bi, Yingzi Zhang, Yiqing Jin, Batoul Maatouk, Niveen M. Khashab, Mo Li
≥1.
Apoptosis assay
The
apoptosis assay
was performed using
Caspase-Glo® 3/7 Assay
System (
Promega
, cat#
G8091
) following the manufacturer’s standard protocol. Briefly, Cas9-sgRNA RNP complexes and ssODNs were delivered
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10/30/2014
Quickly assess cell death- Caspase 3/7 activation
F. Biota
An easy to way to measure cell death in a plate- based assay. This kit allows the scientist to rapidly measure the levels of caspase-3/7 activation in vitro. This assay complements other commonly ...
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Caspase-Glo® 3/7 Assay System From Promega
The Promega Caspase-Glo® 3/7 Assay System is a homogeneous luminescent method used to measure ...
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Caspase-Glo 3/7 Assay from Promega
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