DeadEnd Fluorometric TUNEL System from Promega

Product Specs
ItemDeadEnd Fluorometric TUNEL System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG3250
Quantity60 reactions

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Protective Effect of Brain Derived Neurotrophic Factor-Overexpressing Wharton's Jelly-Derived Mesenchymal Stromal Cells in Severe Intraventricular Hemorrhage in Newborn RatsInternational Journal of Stem CellsFebruary 28, 2028So Yeon Jung, Misun Yang, Young Eun Kim, Dong Kyung Sung, Se In Sung, Chang-Woo Lee, Yun Sil Chang, So Yoon Ahnfluorescence following the manufacturer’s instructions for the DeadEnd Fluorometric TUNEL System kit (G3250; Promega) and counterstained with DAPI. TUNEL-positive cells were observed under a confocal laser scanning

(2) Redox imbalance dictates dependence on GOT1 versus GOT2 for rod photoreceptor health during aging and stressRedox BiologySeptember 1, 2026Meini Chen, Eric Weh, Moloy T. Goswami, Katherine M. Weh, Heather Hager, Peter Sajjakulnukit, Avi J. Weingarten, Shubha Subramanya, Nicholas Miller, Sraboni Chaudhury, et al.were washed and resuspended before staining using the DeadEnd Fluorometric TUNEL kit (Promega, Cat #G3250). Stained samples were analyzed using an Attune NXT flow cytometer (Thermo Fisher Scientific; FITC channel

(3) ALY688 Protects Against Myocardial Ischemia-Reperfusion Injury via Direct Effects and Rab8a-Dependent Extracellular VesiclesJ Extracell VesiclesAugust 29, 2026Hye Kyoung Sung, Jialing Tang, Yubin Lei, Khang Nguyen, Eddie Tam, Jun Wu, Ren-Ke Li, Vincent Richard, Christoph H. Borchers, Lina Antounians, et al.sections were subjected to H&E (Sigma, HT1079, HT110216), Masson Trichrome (Sigma, HT15), TUNEL (Promega, G3250) according to manufacturer's protocol and visualized using a bright field slide scanning microscopy

(4) Klotho-Derived Peptide 1 Protects against Acute Kidney Injury by Directly Targeting Mitochondrial ATAD3AAdv Sci (Weinh)August 25, 2026Xiaoyao Zhang, Shihui Lin, Tianyu Wu, Zhixin Zhang, Hong Zhou, Xue Hong, Youhua Liu(PAS) staining was performed to evaluate the extent of renal tubular injury, while TUNEL staining (G3250; Promega, Madison, WI) was employed to quantify apoptosis. After antigen unmasking, sections were incubated

(5) TSPO Modulation by PIGA-1138 Attenuates Oxidative Stress and Preserves Retinal Function in Experimental Diabetic RetinopathyAntioxidants (Basel)August 12, 2026Alessia Galante, Francesca Corsi, Rosario Amato, Sabrina Taliani, Federico Da Settimo, Maurizio Cammalleri, Ilaria Piano, Massimo Dal Monte, Claudia Garginifor 5 min. Nuclear DNA fragmentation was assessed using the DeadEnd™ Fluorometric TUNEL System (cat #G3250, Promega, Madison, WI, USA), following the manufacturer’s instructions. Nuclei were counterstained with

(6) Neurodevelopmental outcomes relevant to autism in juvenile mice exposed to PCB 11 in the maternal diet throughout gestation and lactationFront ToxicolAugust 12, 2026Rebecca J. Wilson, Harmanpreet K. Panesar, Ilknur Dursun, Rosalia Mendieta, Peter M. Andrew, Xueshu Li, Hans-Joachim Lehmler, Pamela J. Leinstaining TUNEL staining was performed using the DeadEnd Fluorometric TUNEL System (Promega, catalog # G3250; Madison, WI, United States) according to the manufacturer’s instructions using solutions provided in

(7) Genome-Wide Identification of the SRZ Gene Family in Rice and Arabidopsis, and Functional Analysis of OsSRZ1 in Regulating Panicle ExsertionBiology (Basel)August 8, 2026Chao Zhang, Sitian Fei, Yingxiang Hou, Jingjing Xu, Qiannan Sun, Chaofeng Shen, Siyu Liu, Lan Li, Yong Luoparaffin wax [38]. TUNEL labeling was performed using a DeadEnd Fluorometric TUNEL system (Promega, #G3250, Madison, WI, USA) according to the manufacturer’s instructions. 3. Results 3.1. Identification of

(8) Cross-species functional analysis of a de novo DCLK1 variant associated with a neurodevelopmental disorderResearchSquareJuly 23, 2026David F. Butler, Weimin Butler, Hirokazu Hashimoto, Hirokazu Hashimoto, Jonathan H. Sheehan, Jonathan Sheehan, Vanessa Rosenfeld, Lindsay C. Rosenfeld, Lindsay Silverman, Patricia Silverman, et al.assay Apoptotic cells were detected using the Promega DeadEnd™ Fluorometric TUNEL System (Promega Product # G3250) based on the manufacturer’s protocol. Following fixation, cells with permeabilized 0.2% Triton X-100

(9) Long non-coding RNA LOC101926915-encoded micropeptide carboplatin-induced upregulation factor confers carboplatin resistance in ovarian cancerCytojournalJuly 21, 2026Man Li, YingRu Xu, ZhiBiao Mai, Liangliang Ren, Xuexin Yu, SuWen Chen, ZunFu Ke, Ruyuan Yulabeling (TUNEL) assay Apoptotic cells were identified with the DeadEnd ™ Fluorometric TUNEL System (Cat# G3250, Promega, Madison, WI, USA) following the protocol provided by the manufacturer. For paraffin-embedded

(10) Pharmacological modulation of p75 neurotrophin receptor in microglial cells improves resilience to rotenone cytotoxicityFront PharmacolJuly 20, 2026Alessio Valenza, Daniele Pensabene, Francesca Rendina, Maurizio Muzzi, Anna Fracassi, Sandra Moreno, Marco Segattosecondary-electron detection at 5 kV. 2.6 TUNEL assay Detection of apoptotic cells by TUNEL assay (G3250, Promega) was performed as previously reported (Marioli et al., 2024). Following experimental treatments,

(11) Surface-cleaned black phosphorus nanosheets chelate iron and rebalance the ferroptosis-immune axis in acute kidney injuryTheranosticsJuly 20, 2026Wan-Lin Tan, Jin-Ming Zhu, Xiao-Xiao Xu, Jing Jia, Rong-Yu Chen, Xiong Yu, Lu-Qun Liang, Yuan-Yuan Ruan, Fang-Fang Wang, Yu-Ting Chen, et al.terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) Apoptosis Assay Kit (Promega, G3250, USA). After deparaffinization, sections were incubated with 20 μg/mL proteinase K for 15 min at room

(12) GenX exposure induces neurodevelopmental impairment and synaptic toxicity in hESC-derived cerebral organoidsMater Today BioJuly 15, 2026Sung-Ae Hyun, Young-Ju Lee, Moon Yi Ko, Euijun Min, Heejin Park, Younhee Kim, Dae Youn Hwang, Byoung-Seok Lee, Minhan Kadeath in day 20 cerebral organoids was assessed using the DeadEnd™ Fluorometric TUNEL System (Promega, G3250) according to the manufacturer's instructions. Briefly, organoid sections were fixed in 4% formaldehyde

(13) Saccharomyces boulardii CNCM I-745 protects against small intestinal injury in a model of gliadin-peptide-induced sterile inflammatory enteropathyFront ImmunolJuly 6, 2026Aluminé Lezcano, Emanuel Miculan, Geronimo Ducca, Carolina Ruera, María Luz Iribarren, Philippe Tixador, Xavier Roux, Fernando Gabriel Chirdodeparaffinization and rehydration, the sections were treated with the DeadEnd™ Fluorometric TUNEL System (Cat# G3250, Promega) according to the manufacturer’s instructions. Nuclear staining was performed with propidium

(14) A Non-Canonical Role for Hepatocyte MLKL in Promoting Mitochondrial Dysfunction and Senescence in the Aging LiverAging CellJuly 3, 2026Sabira Mohammed, Chao Jiang, Travis Pennington, Shylesh Bhaskaran, Phoebe Ohene-Marfo, Kara F. Kneuper, Constantin Georgescu, Kamille Pitts, Albert Tran, Zongkai Peng, et al.ImageJ. 2.12 TUNEL Assay TUNEL assay was performed using the DeadEnd Fluorometric TUNEL System (#G3250, Promega) and images were taken with Leica SP8 confocal microscope at 200× magnification, 4 random non‐overlapping

(15) Click-crosslinked nanogels integrated into 3D stem cell spheroids enhance regenerative function for swallowing muscle repairBiomaterialsJuly 1, 2026Hideaki Okuyama, Mika Brown, Meghana Munipalle, Sara Nejati, Ran Huo, Hibiki Sakata, Ryosuke Mizuta, Yoshihiro Sasaki, Kazunari Akiyoshi, Hiroe Ohnishi, et al.transferase-mediated dUTP nick end labeling (TUNEL) assay with the DeadEnd Fluorometric TUNEL System kit (Promega; G3250). Spheroids were cross-sectioned on Day 2, 4, and 7, and processed according to the manufacturer’s instructions.

(16) Sex-dependent diabetes impact of acute Grp78 deletion in beta cells of adult miceDiabetesJuly 1, 2026Joshua J.N. Burton, Thalia A. Castro, Alison A. Juray, Hibo A. Hassan, Hillary Diaz Diaz Mosquea, Rohit B. Sharma, Laura C. Alonsoin a citric acid buffer. Apoptotic cells were labeled using The DeadEnd™ Fluorometric TUNEL System (G3250; Promega). Images were acquired using a Zeiss Axio Observer microscope and processed with StrataQuest

(17) The KIF6‐RBP Complex Orchestrates mRNA Transport Required for Sperm Flagellar AssemblyAdvanced ScienceJune 25, 2026Chunbo Xie, Sibing Yi, Xinle Lin, Shen Zhang, Weili Wang, Shimin Yuan, Lanlan Meng, Yong Li, Chen Tan, Chunjia Wei, et al.performed to detect apoptotic germ cells using the DeadEnd Fluorometric TUNEL System kit (Promega, G3250) according to the manufacturer's instructions. Images were captured with an Olympus BX53F fluorescence microscope

(18) Black Phosphorus Nanosheets Penetrate the Blood–Testis Barrier and Induce Reproductive Toxicity in Male MiceEnviron Health (Wash)June 19, 2026Suhua Jiang, Fengkai Ruan, Fucong Zhang, Zewei Long, Xiaoyan Ding, Sihao Zhu, Zhineng Fu, Zhenghong Zuo, Xiaoming Jiang, Chengyong Hethe examination of apoptosis. The TUNEL assay was conducted with a DeadEnd Fluorometric TUNEL System (G3250, Promega, China) in accordance with the specifications outlined by the manufacturer. Apoptotic cells

(19) KLS-3021: Innovative Oncolytic Virotherapy for the Treatment of Advanced Primary and Metastatic Cutaneous Squamous Cell CarcinomaMolecular Therapy OncologyJune 18, 2026Kiwon Park, Jinwon Seo, Sungmin Lee, Hyesoo Kang, Joonsung Kim, Eunjin Lee, Jaeil Shin, Soon-Oh Hong, Sujeong Kim, Sun Jin Kim1:100). The TUNEL assay was performed with the DeadEnd Fluorometric TUNEL System (Promega, cat. no. G3250) according to the manufacturer’s instructions. Following phosphate-buffered saline washes, sections

(20) 17-DMAG-Loaded HER2-Targeted Extracellular Vesicles Induce PARP/Caspase3-Mediated Apoptosis in Gastric CarcinomaInt J Mol SciJune 15, 2026Sin Hye Park, Deok Yong Sim, Do Sang Lee, Chan Mi Lee, Joo Won Moon, Ji Won Choi, Dong Jin Kimassessed using the DeadEndTM Fluorometric TUNEL system kit according to Promega’s instructions (Cat No G3250, Promega, Madison, WI, USA). In brief, NCI-N87 cells treated with 17-DMAG, Ct-Exo17-DMAG, and p51-Exo17-DMAG

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8/11/2015Detecting and Quantifying Apoptotic Cells using DeadEnd™ Fluorometric TUNEL System Lenin VeeravalThis kit was used to detect and quantify the apoptotic cells in a cell population treated with anticancer compounds compared to control cells. The product is simple to use and has high throughput.