With the wide variety of tools currently available and in the works, scientists can assess apoptosis in individual and populations of cells, in samples from live animals to formalin fixed and paraffin embedded (FFPE) microtome slices. Light or fluorescent microscopes, flow cytometers, or spectrophotometers are used for assay detection, and to interrogate parameters ranging from RNA and protein expression, to histone modification, to plasma membrane phospholipid orientation, to membrane potential. The study of apoptosis' defective processes have been associated with a variety of diseases such as cancer. Assay can be outsourced to service facilities or brought in house with custom reagents or pre-made apoptosis assay kits.
New approaches are shedding light on the TME and its impact on disease.
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New technologies are improving accuracy and speed.
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Introducing the LUNA-FX7™ - the automated cell counter that builds on the success of its predecessors. The LUNA-FX7™ is our most powerful cell counter to date, with unmatched cell counting accuracy, a maximum counting volume of 5 µL (10 times that of...
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Light sheet microscopy allows for fast imaging of large 3D biological samples, like whole rodent organs, embryos or larvae. Compared to other current fluorescence microscopy techniques only a single plane of a labeled sample is illuminated ...
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I am using apo one caspase assay kit and it is a very good product and I got good results.
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In this review we evaluate an easy-to-use flow-cytometry-based detection method for apotosis. We use this for a first screening of cell-culture cells.
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