With the wide variety of tools currently available and in the works, scientists can assess apoptosis in individual and populations of cells, in samples from live animals to formalin fixed and paraffin embedded (FFPE) microtome slices. Light or fluorescent microscopes, flow cytometers, or spectrophotometers are used for assay detection, and to interrogate parameters ranging from RNA and protein expression, to histone modification, to plasma membrane phospholipid orientation, to membrane potential. The study of apoptosis' defective processes have been associated with a variety of diseases such as cancer. Assay can be outsourced to service facilities or brought in house with custom reagents or pre-made apoptosis assay kits.
Tips on selection, troubleshooting and deciding between synthetic and natural.
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Interview with Jiye Wang
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Introducing the LUNA-FX7™ - the automated cell counter that builds on the success of its predecessors. The LUNA-FX7™ is our most powerful cell counter to date, with unmatched cell counting accuracy, a maximum counting volume of 5 µL (10 times that of...
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The MACSQuant® Tyto® Cell Sorter brings a gentler, safer, and sterile flow sorting technology to your lab. With the microchip technology at its heart, cells will no longer be exposed to electrical charge, decompression, and fluidic systems....
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I am using apo one caspase assay kit and it is a very good product and I got good results.
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In this review we evaluate an easy-to-use flow-cytometry-based detection method for apotosis. We use this for a first screening of cell-culture cells.
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