With the wide variety of tools currently available and in the works, scientists can assess apoptosis in individual and populations of cells, in samples from live animals to formalin fixed and paraffin embedded (FFPE) microtome slices. Light or fluorescent microscopes, flow cytometers, or spectrophotometers are used for assay detection, and to interrogate parameters ranging from RNA and protein expression, to histone modification, to plasma membrane phospholipid orientation, to membrane potential. The study of apoptosis' defective processes have been associated with a variety of diseases such as cancer. Assay can be outsourced to service facilities or brought in house with custom reagents or pre-made apoptosis assay kits.
Interview with Mahloro Hope Serepa-Dlamini
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New capabilities are making SC investigations more efficient
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The MACSQuant® Tyto® Cell Sorter brings a gentler, safer, and sterile flow sorting technology to your lab. With the microchip technology at its heart, cells will no longer be exposed to electrical charge, decompression, and fluidic systems....
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Discover the beauty of standardized and automated flow cytometry
The MACSQuant® Analyzer 16 is an easy-to-use instrument designed for scalable and reliable flow cytometry assays. This includes features such as:
Full workflow automation: sample ...
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I am using apo one caspase assay kit and it is a very good product and I got good results.
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In this review we evaluate an easy-to-use flow-cytometry-based detection method for apotosis. We use this for a first screening of cell-culture cells.
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