Fig 2: Pro-inflammatory cytokine levels are reduced in the serum of aging Asc−/− mice. Serum IL-6 (A) concentration measured in 2, 6, 12, and 18-month-old WT and Asc−/− mice. Circulating levels of TNF-α (B) and IL-18 (C) determined in 2, 6, 12, and 18-month-old WT, Asc−/−, and Nlrp3−/− mice. n = 4–6 per cohort. ND, Not Determined. Data are presented as mean ± SEM. For each age group, comparisons were made with WT using Student's t-test (A,C) or Mann-Whitney test (A–C) (*p < 0.05, ****p < 0.0001).

Fig 3: Analyses of circulatory cytokines (IFNγ, TNFα, and IL-6) in serum of RD- and MFD-fed control and infected C57BL/6 mice during (a) acute (30 DPI) and (b) chronic (90 DPI) infection by ELISA. The error bars represent standard error of the mean. * p < 0.05, ** p < 0.01, and *** p < 0.001 between indicated groups.

Fig 4: Loss of Cideb protects mice from diet‐induced fatty liver diseases ALiver morphology of WT and Cideb −/− liver under chow diet and HFLF diet. H/E staining, oil red‐O staining, immunohistochemistry, and ultrastructure (Electron Microscope) were performed. Scale bar represented 50 μm in the upper three rows of images and 2 μm in the bottom row of images. LD: lipid droplet; ER: endoplasmic reticulum; M: mitochondrial; N: nucleus.B, CTotal liver TAG levels (B) and liver cholesterol ester levels (C) of WT and Cideb −/− mice under chow diet and HFLF diet (n = 8 per group).DLoss of Cideb inhibits diet‐induced SREBP activation. IB of hepatic SREBP processing and lipogenic enzyme levels (Fasn, Scd1) of WT and Cideb −/− mice under chow diet and HFLF diet.ESerum AST and ALT levels of WT and Cideb −/− mice under chow diet and HFLF diet (n = 8 per group).F Cideb deficiency alleviates the whole body inflammation response. Serum TNFα, MCP1, and IL6 levels of WT and Cideb −/− mice under chow diet and HFLF diet (n = 8 per group).G, HSerum fed glucose levels (G) and insulin levels (H) of WT and Cideb −/− mice under chow diet and HFLF diet (n = 8 per group).IGlucose tolerance test (GTT) of WT and Cideb −/− mice under HFLF diet (n = 5 per group).JInsulin tolerance test (ITT) of WT and Cideb −/− mice under HFLF diet (n = 5 per group).Data information: Data represent Mean ± SEM; NS: not significant; *P < 0.05; **P < 0.01; ***P < 0.001, by 2‐tailed Student's t‐test.Source data are available online for this figure.

Fig 5: POG treatment suppresses inflammatory cytokine expression within LPS-treated RAW264.7 cells. (A) The activity of CCK8 cells was determined to affect RAW264.7 cells within the range of POG (12.5, 25, 50, 100,200 μmol/L). (B–D) qRT-PCR extraction of cellular mRNA for detecting IL-1β, TNF-α, and IL-6 expression. (E–G) iNOS and COX-2 protein levels measured through WB assay. Data were means ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.