IFN-γ Secretion Assay – Detection Kit (PE), human from Miltenyi Biotec

(1) HIV-1 manipulates CD96 on CD4 + T cells to subvert antiviral immunityScience AdvancesSeptember 5, 2025Sandra Dehn, Rabea Burkhard, Johanna Leyens, Tabea Kaiser, Simone Brandimarte, Dinah Heiligensetzer, Herwig Koppensteiner, Baubak Bajoghli, Stephan Hailfinger, Karin Schilbach, et al.secretion assay detection kit PE,Flow cytometry,According to the manufacturer’s protocol,Miltenyi Biotec,130-054-202 IL-2 secretion assay (hum) detection kit PE,Flow cytometry,According to the manufacturer’s protocol,Miltenyi Biotec

(2) UCPVax, a CD4 helper peptide vaccine, induces polyfunctional Th1 cells, antibody response, and epitope spreading to improve antitumor immunityCell Reports MedicineJuly 15, 2025Caroline Laheurte, Laura Boullerot, Babacar Ndao, Marine Malfroy, Lise Queiroz, Phillippe Guillaume, Romain Loyon, Evan Seffar, Eleonore Gravelin, Adeline Renaudin, et al.Isolation Kit,Miltenyi,Cat#130-096-553 "IFN-γ Secretion Assay – Detection Kit (PE), human",Miltenyi,Cat#130-054-202 Dynabeads mRNA Purifications Kits,ThermoFisher,Cat#A33562 Human IFN gamma ELISpot Set,Diaclone,Cat#856051020P ELISpot

(3) A CD4+ T cell-intrinsic complement C5aR2-prostacyclin-IL-1R2 axis orchestrates Th1 cell contractionImmunityJune 10, 2025Jubayer Rahman, Jack A. Bibby, Parul Singh, Nicolas S. Merle, Erin E. West, Andrea Bohrer, Katrin Mayer-Barber, Chengyu Liu, Lauren R. Brinster, Behdad Afzali, et al.Regulatory T Cell Isolation Kit,Stem Cell Technologies,Cat# 18063 IFN-γ Secretion Assay,Miltenyi Biotec,Cat# 130-054-202 IL-10 Secretion Assay,Miltenyi Biotec,Cat# 130-090-761 LEGENDplex Human T helper cytokine panel,Biolegend,Cat#

(4) Coronavirus replicase epitopes induce cross-reactive CD8 T cell responses in SARS-CoV-2-naive people with HIV-1iScienceMarch 21, 2025Katja G. Schmidt, Paulina Geißler, Ev-Marie Schuster, Christine Schülein, Ellen G. Harrer, Verena Schönau, Markus Luber, Bernd Spriewald, Philipp Steininger, Silke Bergmann, et al.commercial assays ,, "IFN-γ Secretion Assay – Detection Kit (PE), human, for 100 tests",Miltenyi,Cat#130-054-202 SARS-CoV-2 Spike IgG ELISA,Euroimmun,Cat#EI 2606–9601 G SARS-CoV-2 NCP IgG ELISA,Euroimmun,Cat#EI

(5) Systematic single-cell pathway analysis to characterize early T cell activationCell reportsNovember 22, 2022Bibby JA, Agarwal D, Freiwald T, Kunz N, Merle NS, West EE, Singh P, Larochelle A, Chinian F, Mukherjee S, et al.hybridoma, Anti-human CD28,eBioscience,Cat#16-0289-81; RRID:AB_468926 Human IFNγ-APC secretion assay,Miltenyi,Cat#130-054-202 ,, Bacterial and virus strains,Bacterial and virus strains,Bacterial and virus strains ,, Biological

(6) Identification and assessment of TCR-T cells targeting an epitope conserved in SARS-CoV-2 variants for the treatment of COVID-19International immunopharmacologyNovember 1, 2022Ma Y, Liu F, Li B, Peng K, Zhou H, Xu Y, Qiao D, Deng L, Tian G, Nielsen M, et al.BD Biosciences, 555838), APC-CD107a (clone H4A3, BD Biosciences, 560664), PE-IFN-γ (Miltenyi Biotec, 130-054-202) and 7-AAD (BD Biosciences, 559925) for removal of dead cells. The stained cells were washed and re-suspended

(7) Distinct phenotypic states and spatial distribution of CD8(+) T cell clonotypes in human brain metastasesCell reports. MedicineMay 17, 2022Sudmeier LJ, Hoang KB, Nduom EK, Wieland A, Neill SG, Schniederjan MJ, Ramalingam SS, Olson JJ, Ahmed R, Hudson WH.assays,Critical commercial assays ,, "IFNγ Secretion Assay – Detection Kits, human",Miltenyi,Cat# 130-054-202 TCR profiling (of circulating CD8+ T cells),Adaptive Biotechnologies,Human TCRB immunoSEQ Visium Spatial Gene Expression

(8) Rapid Generation of TCR and CD8αβ Transgenic Virus Specific T Cells for Immunotherapy of LeukemiaFrontiers in ImmunologyApril 29, 2022Gagan Bajwa, Caroline Arbersecreting cells were isolated using the IFN-γ secretion assay-cell enrichment kit (Miltenyi Biotech, #130-054-202) according to the manufacturer’s recommendations (Figure 1B). IFN-γ secreting cells were plated in complete

(9) Transcriptomics of Acute DENV-Specific CD8+ T Cells Does Not Support Qualitative Differences as Drivers of Disease SeverityVaccines (Basel)April 14, 2022Alba Grifoni et al.37 °C. IFNγ producing cells were labeled using an IFN-γ Secretion Assay–Detection kit (PE) (catalog #130-054-202, Miltenyi Biotec, Bergisch Gladbach, North Rhine-Westphalia, Germany) according to manufacturer’s instructions.

(10) Rapid identification of CMV-specific TCRs via reverse TCR cloning system based on bulk TCR repertoire dataFrontiers in immunologyJanuary 1, 2022Hong CH, Pyo HS, Baek IC, Kim TG.PBMCs captured IFN-γ-secreting cells by using IFN-γ Secretion Assay Detection Kits (Miltenyi; cat. no. 130-054-202) according to the manufacturer’s instructions and stained with anti-CD3-BV421, anti-CD8α-APC-Cy7, and

(11) Heterogeneity of human anti-viral immunity shaped by virus, tissue, age, and sexCell reportsNovember 30, 2021Poon MML, Byington E, Meng W, Kubota M, Matsumoto R, Grifoni A, Weiskopf D, Dogra P, Lam N, Szabo PA, et al.24hrs were first stained using the IFN-γ Secretion Assay – Detection Kit, human (Miltenyi Biotec, cat# 130-054-202), which allows isolation of intact IFN-γ-secreting cells using an IFN-γ catch reagent that attaches

(12) Common clonal origin of conventional T cells and induced regulatory T cells in breast cancer patientsNature communicationsFebruary 18, 2021Xydia M, Rahbari R, Ruggiero E, Macaulay I, Tarabichi M, Lohmayer R, Wilkening S, Michels T, Brown D, Vanuytven S, et al.effector CD4+ TC (Teff) were identified using the IFNγ Secretion Assay- Detection Kit (PE) human (cat. 130-054-202, Miltenyi Biotec) according to the manufacturer’s recommendations with slight modifications. In particular,

(13) Dengue-specific CD8+ T cell subsets display specialized transcriptomic and TCR profilesJournal of Clinical InvestigationMarch 18, 2019Yuan Tian et al.stimulation. IFN-γ–producing cells were labeled using an IFN-γ Secretion Assay – Detection Kit (catalog 130-054-202, Miltenyi Biotec) according to the manufacturer’s instructions. Subsequently, PBMCs were stained with

(14) The Culture Dish Surface Influences the Phenotype and Cytokine Production of Human Monocyte-Derived Dendritic CellsFrontiers in immunologyJanuary 1, 2019Sauter A, Yi DH, Li Y, Roersma S, Appel S.University of Bergen. IFN-γ Secretion Assay An IFN-γ secretion assay (Miltenyi Biotec, catalog number 130-054-202) was utilized to analyze the capacity of the generated DC populations to induce antigen specific T cell

(15) Impact of human mesenchymal stromal cells on antifungal host response against Aspergillus fumigatusOncotargetNovember 10, 2017Schmidt S, Tramsen L, Schneider A, Schubert R, Balan A, Degistirici Ö, Meisel R, Lehrnbecher T.

(16) CD1b-mycolic acid tetramers demonstrate T-cell fine specificity for mycobacterial lipid tailsEuropean journal of immunologySeptember 1, 2017Van Rhijn I, Iwany SK, Fodran P, Cheng TY, Gapin L, Minnaard AJ, Moody DB.

(17) Ex vivo analysis of resident hepatic pro-inflammatory CD1d-reactive T cells and hepatocyte surface CD1d expression in hepatitis CJournal of viral hepatitisAugust 1, 2013Yanagisawa K, Yue S, van der Vliet HJ, Wang R, Alatrakchi N, Golden-Mason L, Schuppan D, Koziel MJ, Rosen HR, Exley MA.Inc. CD1d 12-0016 eBioScience, Inc. CD8 25-0088 eBioScience, Inc. CD69 12-0699 eBioScience, Inc. IFNγ Capture bi-mAb 130-054-202 Miltenyi BioSciences, Inc. NIH-PA Author Manuscript NIH-PA Author Manuscript J Viral Hepat. Author

(18) HIV-1-specific cytotoxicity is preferentially mediated by a subset of CD8(+) T cells producing both interferon-gamma and tumor necrosis factor-alphaBloodJuly 15, 2004Lichterfeld M, Yu XG, Waring MT, Mui SK, Johnston MN, Cohen D, Addo MM, Zaunders J, Alter G, Pae E, et al.

(19) Immune responses in healthy and allergic individuals are characterized by a fine balance between allergen-specific T regulatory 1 and T helper 2 cellsThe Journal of experimental medicineJune 7, 2004Akdis M, Verhagen J, Taylor A, Karamloo F, Karagiannidis C, Crameri R, Thunberg S, Deniz G, Valenta R, Fiebig H, et al.

(20) An immunodominant SSX-2-derived epitope recognized by CD4+ T cells in association with HLA-DRThe Journal of clinical investigationApril 1, 2004Ayyoub M, Hesdorffer CS, Montes M, Merlo A, Speiser D, Rimoldi D, Cerottini JC, Ritter G, Scanlan M, Old LJ, et al.

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Miltenyi Biotec’s IFNγ Secretion Assay Detection Kit (PE)
The MACS IFNg Secretion Assay Detection Kit is designed for the analysis of IFN-gamma (IFNg) ... Read More

IFN-γ Secretion Assay – Detection Kit (PE), human from Miltenyi Biotec

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Miltenyi Biotec’s IFNγ Secretion Assay Detection Kit (PE)

IFN-γ Secretion Assay – Detection Kit (PE), human from Miltenyi Biotec

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