Mouse TNF-alpha DuoSet ELISA DY410-05 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMouse TNF-alpha DuoSet ELISA
CompanyR&D Systems, a Bio-Techne Brand
Price $450.00Supplier Page View Company Product Page
Catalog NumberDY410-05
Quantity1 Kit (for 5 Plates) (also 1 Kit (for 15 Plates))
ApplicationsELISA
Detection Range31.20 - 2000 pg/mL
ReactivityMouse
Detection TargetTNF-alpha
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene Nametumor necrosis factor
NCBI Gene AliasesDIF, TNF-a, TNF-alpha, TNFSF2, TNFalpha, Tnfa, Tnfsf1a, Tnlg1f

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) ZBP1 mediated restriction of Toxoplasma gondii in both the early acute and chronic brain stages of infectionImmunohorizonsApril 17, 2026Lydia Anne Sibley, Isaac William Babcock, Naa Dedee Amadi, Anne Elise Marchildon, Abigail Grace Kelly, Sydney Ann Labuzan, John Robert Lukens, Tajie Heartsong HarrisCat#M1240]) and DuoSet ELISA (Mouse IFN-gamma [R&D Systems; Cat# DY485], Mouse TNF-alpha [R&D Systems; Cat#DY410-05], Mouse IL-1 alpha/IL-1F1 [R&D Systems; Cat #DY400-05], Mouse IL-18 [R&D Systems; Cat# DY7625-05]) with

(2) Strain-specific persistence of Burkholderia cenocepacia in the C3HeB/FeJ mouse model of pulmonary infectionJ Med MicrobiolApril 9, 2026Ziting Chen, Mariana Romero-Gonzalez, Andrea Maser, Ruisi Leng, Lindsay J. Caverly, Marc Hershenson, Ashlee D. Brunaughand stored at −80 °C. TNF-α levels were quantified using a murine TNF-α ELISA kit (R and D Systems, DY410-05) following the manufacturer’s instructions. Absorbance was measured at 450 nm, and cytokine concentrations

(3) Time Course Characterization of Protective Immune Responses Following BCG Vaccination in BALB/c MicePathogensApril 6, 2026Hee Ho Kim, Kwangwook Kim, Min Jung Kim, Ye Jin Yang, Eun Bee Choi, Ji Woong Heo, Seo Young Moon, Heeji Lim, Yookyoung Lee, In-Ohk Ouh, et al.(pg/mL) using a commercially available ELISA kit (R&D Systems, Minneapolis, MN, USA; DY402-05 for IL-2; DY410-05 for TNF-α; and DY485-05 for IFN-γ) according to the manufacturer’s instructions. 2.4. qPCR Analysis Total

(4) Decanoic acid modulates cerebral metabolism and attenuates ischemic injury in a mouse modelMetab Brain DisMarch 28, 2026Antonio Ítalo Santos Nunes, Lurian Caetano David, Daniel Pereira Cavalcante, Gustavo Almeida de Carvalho, Eduardo Rosa Silva, Jacqueline Alves Leite, Nelson Roberto Antoniosi Filho, Mauro Cunha Xavier Pintoranging from 15.6 to 2000 pg/mL. Final values were expressed in pg/mL for TNF-α (R&D Systems, Cat# DY410-05, RRID: not available), IL-1β (R&D Systems, Cat# DY401-05, RRID: not available), and IL-6 (R&D Systems,

(5) Improved immune responses and tuberculosis protection by aerosol vaccination with recombinant BCG expressing ESX-1 from Mycobacterium marinumPLOS PathogensFebruary 23, 2026Fadel Sayes, Wafa Frigui, Alexandre Pawlik, Cécile Tillier, Magali Tichit, David Hardy, Roland BroschDuoSet ELISA kits (R&D Systems) were used for mouse IL-1β/IL-1F2 (DY401–05), IL-6 (DY406–05) and TNF-α (DY410–05) quantifications, in duplicate, in 100 µl volume per sample using Nunc 96-well Maxisorp plates and the

(6) Insights into Bioactive Constituents from Pericarp of Garcinia mangostana: Anti-Inflammatory Effects via NF-κB/MAPK Modulation and M1/M2 Macrophage PolarizationAntioxidants (Basel)January 19, 2026Cheng-Shin Yang, Sin-Min Li, Jih-Jung ChenNaNO2. 2.7. Evaluation of Inflammatory Cytokine The production of IL-6 (R&D #DY406-05), TNF-α (R&D #DY410-05), and IL-10 (R&D #DY417-05) was quantified using the commercial ELISA kits (R&D Systems, Minneapolis,

(7) Mucosally sourced complement factor B modulates the host response to colitisbioRxivJanuary 3, 2026Aayusha Thapa, Aasritha Nallapu, Nnenna Mougboh, Swathi Nedunchezian, Khushi Talati, Brian Yang, Jungheun Hyun, John Michael Sanchez, Christopher S. Seet, Hon Wai Koon, et al.for 10,000g for 30 min at 4 °C. Supernatants were collected and stored at −20 °C. TNF-α (R&D Systems #DY410–05), MPO (R&D Systems DY3667) and IL-1β (DY401) levels were measured by following the manufacturer’s instructions.

(8) Integrative Network Pharmacology and Multi-Omics Analysis Reveal Key Targets and Mechanisms of Saikosaponin B1 Against Acute Lung InjuryMetabolitesDecember 4, 2025Yuanfei Niu, Meiting Liu, Shuang Cui, Kaiyang Liu, Mengyuan Yang, Xiaozhen Hu, Changhui Zheng, Lianmei Wang, Junling CaoBio-Technology Co., Ltd. (Shanghai, China). Murine ELISA kits for IL-6 (DY406-05), IL-1β (DY401-05), and TNF-α (DY410-05) were obtained from R&D SYSTEMS (Minneapolis, MN, USA). 2.13. H&E Staining Lung tissues were fixed

(9) Characterizing the mechanisms underpinning interleukin-15Rα-mediated protection against sepsis and candidiasisCytokineDecember 1, 2025Yang J, Xu B, Cao J, Liu Y, Tang L, Zhao P, Li S, Li X, Liu J, Yu R, et al.

(10) Forced polarisation of microglia by IL-13 is modified by inflammatory and microenvironmental contextInflammation ResearchDecember 1, 2025Emmanuelle D. Aiyegbusi, James P. Reynolds, Ross O’Carroll, Ruth Colbert, Christopher Carew, Dearbhaile Dooleywere determined using ELISA according to the instructions provided by the manufacturer (Bio-Techne, DY410-05).The culture supernatant was collected from the wells of a 6 or 24 well plate that had been seeded with

(11) Methyl jasmonate elicitation enhances photoprotective and anti-inflammatory properties in Lavandula angustifolia cell suspension culturesScientific ReportsNovember 7, 2025Dong-Ho Bak, Bo Ryeong Kim, Ki-Hyun Kim, Gyeongchan Jeon, Yu Jeong Jeong, Soyoung Kim, Ok Ran Lee, Jae Cheol Jeong, Cha Young Kimthe culture supernatants were measured using enzyme-linked immunosorbent assay (ELISA) kits (TNF-α: DY410-05; IL-6: DY406-05; R&D Systems, Inc., Minneapolis, MN, USA), following the manufacturer’s protocols. Absorbance

(12) Localised delivery of interleukin-13 from a PLGA microparticle embedded GelMA hydrogel improves functional and histopathological recovery in a mouse contusion spinal cord injury modelBioactive materialsNovember 1, 2025Walsh CM, Colbert R, Reynolds JP, Dunne E, Aiyegbusi ED, O'Carroll R, Wychowaniec JK, Masuda T, Knobeloch KP, Prinz M, et al.

(13) Inhalability and Bioactivity of Spray‐Dried versus Pressurized Gas eXpanded Liquid Technology‐Processed Yeast Beta‐Glucan Microparticles for Anti‐Fibrotic TherapiesAdvanced Healthcare MaterialsNovember 1, 2025Nate Dowdall, Safaa Naiel, Quan Zhou, Pareesa Ali, Tamana Rubina Yousof, Emily Wong, Ricardo Couto, Byron Yépez, Bernhard Seifried, Paul Moquin, et al.(CS‐93668). Commercially available enzyme‐linked immunosorbent assay (ELISA) kits for mouse TNF‐α (Cat# DY410‐05) and IL‐1β (Cat# DY401‐05) were purchased from BioTechne R&D systems (Minnesota USA). Laboratory grade

(14) Proteolytic dysfunction in bovine digital dermatitis caused by Treponema spp. and the therapeutic use of non-antimicrobial matrix metalloproteinase inhibitorsCommun BiolOctober 29, 2025Priyoshi Lahiri, Daniel Young, Regan Penner, Kyle Lesack, Angelica Petersen Dias, Rita Hannawayya, Jeroen De Buck, Karin Orsel, Morley D. Hollenberg, Francis Johnson, et al.cultured. Secretions of murine Tnf-α, Il-1β and Cxcl-1 were quantified in cell supernatants by ELISAs (DY410-05, DY410-05, DY452-05 and DY453-05, respectively; R&D Systems). Bovine Il-1β was determined in cell supernatants

(15) Immune subversion by Leishmania infantum parasites suppresses NLRP3-driven inflammatory responses in amyloid-β-activated microgliaJournal of neuroinflammationOctober 29, 2025Calvo Alvarez E, Sfogliarini C, La Rosa F, Saresella M, Dolci M, Vegeto E, Taramelli D, Basilico N, Clerici M.

(16) Ceftriaxone attenuates Poly I:C–induced neuroinflammation in vitro by modulating glutamate transport, synaptic integrity, and immunometabolic reprogrammingFrontiers in Cellular NeuroscienceOctober 28, 2025Xizi Shi, Yesheng Sun, Shirin Hosseini, Fangfang Chen, Thekla Cordes, Kristin Michaelsen-Preusse, Martin Kortelevels of the cytokines TNF-α, IL-6, and IL-10 were quantified using ELISA kits (R&D Systems: TNF-α DY410-05, IL-6 DY406-05, IL-10 DY417-05). No other cytokines were measured. Culture medium was collected from

(17) Bivalent mRNA vaccine booster enhances immunity against XBB.1.5 more effectively than breakthrough infection in K18-hACE2 miceiScienceOctober 17, 2025Marissa E. Linger, Sytze H.T. Jorritsma, Jonna Bloeme-Ter Horst, Jessika C. Zevenhoven-Dobbe, Finn Rijlaarsdam, Emil Colstrup, Macha Beijnes, Jutte J.C. de Vries, Ramon Arens, Rajagopal Murugan, et al.wells were set for each sample. ELISA kits specific for mouse IFN-β (DY8234-05, R&D Systems), TNF-α (DY410-05, R&D Systems), IL-6 (DY406-05, R&D Systems), IFN-γ (D485-05, R&D Systems), CXCL10 (DY466-05), IL-10

(18) SIRT7 inhibits cerebral ischemic injury by inhibiting microglia M1 polarization via desuccinylation of NAMPTBrain research bulletinOctober 15, 2025Cheng Y, Zhao K, Li J, Lei Q, Zhang G, Gao X.

(19) The Influence of polycyclic aromatic hydrocarbons exposure on the gut microbiome composition and inflammatory responsesEcotoxicology and environmental safetySeptember 15, 2025Jeong SH, Jung J, Park YJ, Lee SJ, Lee SJ.

(20) SIFD-associated TRNT1 deficiency unveils importance of TSPO during macrophage antibacterial and antiviral responsesFrontiers in ImmunologySeptember 11, 2025Duale Ahmed, Angelo Slade, Thet Fatica, Stephen Baird, Krishna Bhattarai, Thérèse Atallah, Edana Cassol, Martin HolcikC2920) were acquired from Sigma-Aldrich. IL-1β (Cat#: DY401-05), IL-6 (Cat#: DY406-05), TNF (Cat#: DY410-05), IFN-β (Cat#: DY8234-05) DuoSet ELISA kits were purchased from R&D Systems. Tetramethylrhodamine, methyl

Write a Review

(1)

11/2/2022Extremely Reliable and Clean Results with TNF-alpha ELISA stephen ChetwyndUsed to study the Zymosan induced TNFa response from isolated mouse neutrophils. Saw robust and reproducible response across many experiments using this kit.
(1)

8/25/2017TNF-alpha ELISA Kit Juwen DuBoisWe wanted to determine whether there were differences in TNF-a protein in mouse brains in 2 different mouses models after treatment with a chemical that induces demyelination.
(1)

5/31/2016DuoSet Mouse TNF-Alpha Joanna KreitingerIn vivo experiments using transgenic mice were performed to establish the role of a specific transcription factor during a systemic immune response.
(1)

3/1/2016Reliable and Easy to Use ELISADr. Jacqueline KimmeyThis was used to analyze TNF-a levels in murine lung homogenate following infection with M. tuberculosis. Assay is simple to use and gives reliable and consistent results

TNF-alpha ELISA

Mouse TNF-alpha DuoSet ELISA

Mouse TNF-alpha DuoSet ELISA from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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Mouse TNF-alpha DuoSet ELISA from R&D Systems, a Bio-Techne Brand

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