GSH-Glo Glutathione Assay V6912 from Promega

Product Specs
ItemGSH-Glo Glutathione Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV6912
Detection TargetGlutathione
MethodAssay
Quantity50ml

Specifications/Features

GenBank / EMBL Accession number : DQ904461

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Repurposing loratadine to induce ferroptosis and overcome multidrug resistance: preclinical evidence in KB-V-1 cellsFrontiers in OncologyJune 17, 2026Nicholas Cook, Xin Li, Nathan J. Bowen, Alira Danaher, MaKayla Thomas, Saani Alhassan, Briah Arms, Daqing Wucontrol group. Intracellular glutathione assay Intracellular GSH levels were measured using the GSH-Glo™ Glutathione Assay (V6912, Promega, Madison, WI) according to the manufacturer’s instructions. KB-V-1 cells were treated with

(2) Sulfur partitioning from cysteine controls T cell proliferation and effector functionCellMarch 31, 2026Beth Kelly, Minsun Cha, Tatjana Gremelspacher, Jacob L. Martin, Massimo Andreis, Isha Maloo, Gustavo E. Carrizo, Mia Gidley, Michal A. Stanczak, Petya Apostolova, et al.kit,Qiagen,Cat# 27106 ATP determination kit,Invitrogen,Cat# A22066 GSH-Glo glutathione assay,Promega,Cat# V6912 NAD/NADH-Glo assay,Promega,Cat# G9071 Gamma glutamyl transferase (GGT) assay kit (fluorometric),abcam,Cat#

(3) Catabolism of extracellular glutathione supplies cysteine to support tumoursNatureMarch 18, 2026Fabio Hecht, Marco Zocchi, Emily T. Tuttle, Nathan P. Ward, Fatemeh Alimohammadi, Amal Afzal Khan, Veronica C. Gomes, Bradley Smith, Jennifer J. Twardowski, Bradley N. Mills, et al.some experiments, the GSH concentration was determined using a GSH-Glo Glutathione Assay (Promega, V6912). In brief, 6,250 cells were seeded into 96-well white cell culture-treated plates (Falcon, 353296),

(4) Isocitrate dehydrogenase 1 primes group-3 medulloblastomas for cuproptosisCancer CellJune 9, 2025Derek Dang, Akash Deogharkar, John McKolay, Kyle S. Smith, Pooja Panwalkar, Simon Hoffman, Wentao Tian, Sunjong Ji, Ana P. Azambuja, Siva Kumar Natarajan, et al.CellTiter-Fluor™ Cell Viability Assay,Promega,Cat#G6082 CellTiter-Glo® 2.0 Cell Viability Assay,Promega,Cat#G9242 GSH-Glo™ Glutathione Assay,Promega,Cat #V6912 Pierce BCA Protein Assay Kit,ThermoFisher,Cat #23225 Mission siRNA Transfection Reagent,Sigma-Aldrich,Cat#

(5) CBX3 promotes multidrug resistance by suppressing ferroptosis in colorectal carcinoma via the CUL3/NRF2/GPX2 axisOncogeneMarch 16, 2025Xiaoming Bai, Tinghong Duan, Jiaofang Shao, Yutong Zhang, Guangyuan Xing, Jie Wang, Xue Liu, Min Wang, Yuanqiao He, Hai Wang, et al.treatments were added to each well. GSH levels were evaluated using the GSH-Glo assay kit (Promega, V6912), following manufacturer instructions. To detect total glutathione (GSH + GSSG), the reducing agent

(6) Suppression of the LKB1-AMPK-SLC7A11-GSH signaling pathway sensitizes NSCLC to albumin-bound paclitaxel via oxidative stressRedox BiologyFebruary 25, 2025Dade Rong, Liangliang Gao, Yiguan Chen, Xiang-Zheng Gao, Mingzhu Tang, Haimei Tang, Yuan Gao, Guang Lu, Zhi-Qiang Ling, Han-Ming ShenHY-112769) for 24 h. Glutathione levels were quantified using the GSH-Glo™ Glutathione Assay kit (Promega, V6912) according to manufacturer's instructions. Total levels of glutathione were normalized to protein concentrations

(7) Quantitative adverse outcome pathway modeling for cigarette-smoke-induced airway mucus hypersecretion. Part 1: adverse-outcome-pathway-based in vitro assessment with repeated exposure to whole cigarette smokeFront ToxicolJanuary 1, 2025Ichikawa S, Muratani S, Sano K, Erami K, Mori A, Matsumoto R, Ito S.The GSH levels in the lysates were measured using the GSH-Glo Glutathione Assay (Promega, cat. no. V6912) as per manufacturer instructions. The phosphorylated EGFR levels in the lysates were measured using

(8) Increasing sustainability and reproducibility of in vitro toxicology applications: serum-free cultivation of HepG2 cellsFrontiers in ToxicologyNovember 22, 2024Luisa Marie Pfeifer, Janike Sensbach, Frederic Pipp, Daniela Werkmann, Philip Hewitttreatment, the GSH-Glo™ Glutathione Assay from Promega was used according to manufacturer’s instructions (V6912). HepG2 cells cultivated in standard medium containing FBS, Advanced DMEM/F12 or TCM® Serum Replacement

(9) Melatonin and erastin emerge synergistic anti-tumor effects on oral squamous cell carcinoma by inducing apoptosis, ferroptosis, and inhibiting autophagy through promoting ROSCellular & Molecular Biology LettersMay 2, 2023Leilei Wang, Chuan Wang, Xuan Li, Zhuoying Tao, Wangyong Zhu, Yuxiong Su, Wing Shan Choifor 48 h. GSH levels were measured using the GSHGlo Glutathione Assay kit (Promega, Madison, WI, USA, V6912), following the manufacturer’s instructions. Luminescence was measured using an LMax Microplate Reader

(10) Moringa oleifera Lam Leaf Extract Stimulates NRF2 and Attenuates ARV-Induced Toxicity in Human Liver Cells (HepG2)Plants (Basel)April 3, 2023Siqiniseko S. Ndlovu, Anil A. Chuturgoon, Terisha Ghaziand JC-1 monomers [120]. 4.6. Antioxidants Assays 4.6.1. Glutathione Assay The GSH-Glo™ assay (V6912, Promega, Madison, WI, USA) was used to quantify total and reduced glutathione. Cells were transferred

(11) Streptococcus pyogenes Hijacks Host Glutathione for Growth and Innate Immune EvasionmBioJune 28, 2022Stephan Brouwer, Magnus G. Jespersen, Cheryl-lynn Y. Ong, David M. P. De Oliveira, Bernhard Keller, Amanda J. Cork, Karrera Y. Djoko, Mark R. Davies, Mark J. Walker(NAD+/reduced NADH), NADP (oxidized NADP+/reduced NADPH) redox couples using GSH-Glo (Promega; catalog no. V6912), NAD/NADH-Glo (Promega; catalog no. G9071) and NADP/NADPH-Glo (Promega; catalog no. G9081) assays as

(12) LncCCAT1 interaction protein PKM2 upregulates SREBP2 phosphorylation to promote osteosarcoma tumorigenesis by enhancing the Warburg effect and lipogenesisInternational Journal of OncologyMarch 2, 2022Feifei Pu et al.instructions. Glutathione (GSH) measurement GSH levels were determined using the GSH-Glo™ assay (cat. no. V6912, Promega Corporation). Fluorescein derivatives were converted to fluorescein in the presence of GSH.

(13) YAP/TAZ and ATF4 drive resistance to Sorafenib in hepatocellular carcinoma by preventing ferroptosisEMBO Molecular MedicineDecember 7, 2021Ruize Gao et al.counter. Intracellular GSH levels were measured using the GSH‐Glo Glutathione Assay kit (Promega V6911/V6912) and a bioluminescence plate reader (Berthold Centro LB 960). The results were normalized to cell numbers. Cystine

(14) Glutathione peroxidase 4‐dependent glutathione high‐consumption drives acquired platinum chemoresistance in lung cancer‐derived brain metastasisClinical and Translational MedicineSeptember 26, 2021Wenwen Liu, Yang Zhou, Wenzhe Duan, Jing Song, Song Wei, Shengkai Xia, Yingyan Wang, Xiaohui Du, Encheng Li, Caixia Ren, et al.to attach for 12 hours. Glutathione (GSH) level was evaluated using the GSH‐Glo assay kit (Promega, V6912) according to the manufacturer's instructions. To detect total glutathione (GSH + GSSG), reducing agent

(15) Structure‐Activity‐Relationship‐Aided Design and Synthesis of xCT Antiporter InhibitorsChemMedChemSeptember 6, 2021Davide Cirillo, Shahin Sarowar, Per Øyvind Øyvind Enger, Hans‐René Bjørsvikintracellular Glutathione that was performed by the glutathione‐Glo assay (Promega, Madison, WI, USA, cat#V6912) according to the manufacturer's recommended instruction. A flat bottomed 96‐well plate was seeded with

(16) Prophage exotoxins enhance colonization fitness in epidemic scarlet fever-causing Streptococcus pyogenesNature CommunicationsDecember 1, 2020Stephan Brouwer et al.measured spectrophotometrically at 490 nm. GSH release was measured using the GSH-Glo GSH assay (Promega; V6912), as per the manufacturer’s instructions, with the modification of mixing undiluted samples 1:1 with

(17) Cancer-associated fibroblasts-derived gamma-glutamyltransferase 5 promotes tumor growth and drug resistance in lung adenocarcinomaAging (Albany NY)July 8, 2020Jia-Ru Wei, Jun Dong, Lei Li(http://rsb.info.nih.gov/ij). GSH content and GGT activity assay The GSH-Glo™ Glutathione Assay Kit (#V6912, Promega) was used to determine the reduced GSH content in the different medium samples. γ-Glutamyltransferase

(18) Increased RLIP76 expression in IDH1 wild‑type glioblastoma multiforme is associated with worse prognosisOncology reportsJanuary 1, 2020Wang Q, Zhang L, Cui Y, Zhang C, Chen H, Gu J, Qian J, Luo C.score 0). Quantification of GSH GSH was quantified using a GSH-Glo™ Glutathione Assay kit (cat. no. V6912; Promega Corporation), according to the manufacturers' instructions, following incubation of 1×104 cells/well

(19) IDH1 R132H Mutation Enhances Cell Migration by Activating AKT-mTOR Signaling Pathway, but Sensitizes Cells to 5-FU Treatment as NADPH and GSH Are ReducedPloS oneJanuary 1, 2017Zhu H, Zhang Y, Chen J, Qiu J, Huang K, Wu M, Xia C.× 100%. Quantification of GSH GSH and GSSG were quantified using a GSH-Glo™ Glutathione Assay Kit (V6912, Promega) according to the manufacturers’ instructions, following incubation of 1×104 cells/well in 96-well

(20) Glutathione Preservation during Storage of Rat Lenses in Optisol-GS and Castor OilPLoS ONENovember 19, 2013Thomas Holm, Martin Rocho Brøgger-Jensen, Leif Johnson, Line Kesselluminescence detection kit according to the manufacturer's instructions (Glutathione assay kit, Promega V6912). The kit exhibits a high specificity for reduced glutathione rather than thiols in general. Oxidized

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10/27/2026Sensitive, Plate-Based GSH Readout in Under an Hour Wei Yang SitThe Promega GSH-Glo Glutathione Assay is used to quantify reduced glutathione in cultured cells, and it is a much faster alternative to HPLC or classic colorimetric/fluorometric thiol assays.

GSH-Glo Glutathione Assay

GSH-Glo Glutathione Assay from Promega

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Specifications/Features

Promega

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GSH-Glo Glutathione Assay from Promega

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