Tumor Dissociation Kit, mouse 130-096-730 from Miltenyi Biotec

(1) Prednisolone modulates CD8⁺ and regulatory T-cell activity to dampen response to immune checkpoint inhibitor therapy in melanomaOncoimmunologyDecember 31, 2026Jesse R. Brown, Bernadette Pedersen, Georgina V. Long, Nigel G. Maher, Su Yin Lim, Helen Rizos, Elena Shklovskayaexcised, mechanically dissociated, enzymatically digested using the Mouse Tumor Dissociation Kit (Cat# 130-096-730, Miltenyi Biotec, Bergisch Gladbach, Germany) and passaged through a 70 µm stainless-steel mesh to obtain

(2) Single-cell analysis highlights the significance of malignant cell IFN/MHC-II for immunotherapy response in head and neck squamous cell carcinomaCell Reports MedicineApril 21, 2026Michael Mints, Reilly A. Sample, Anuraag S. Parikh, Jesse M. Zaretsky, Zongtai Qi, Travis Law, Fudong Wang, Thomas F. Barrett, Riley Mullins, Ashley Reeb, et al.with PBS (Thermo Fisher), and dissociated using the Mouse Tumor Dissociation Kit (Miltenyi Biotec, 130-096-730) per manufacturer protocol. Dissociated cell suspensions were passed through a 70 μm strainer (MIDSCI,

(3) Targeting the noncanonical function of metabolic enzyme PHGDH in driving PD-L1 expression and cancer immune evasionCell Reports MedicineApril 21, 2026Juan Liu, Weiwei Wang, Yvonne Sun, Shan Huang, Arnav Borole, Haiyan Zheng, Wenwei Hu, Zhaohui FengN8080234 PHGDH Activity Assay Kit,Abcam,Cat# ab273328 Tumor Dissociation Kit,Miltenyi Biotec,Cat# 130-096-730 LDH release assay,Abcam,Cat# ab65393 CD8+ T cell isolation Kit,Miltenyi Biotec,Cat# 130-104-075 MycoAlert

(4) KSR2 functions as a metabolic checkpoint for anti-PD-1 resistance by reprogramming glucose metabolismCancer Immunol ImmunotherApril 21, 2026Yuli Ge, Qiong Zhou, Qiangqiang Zhang, Yanyan Hu, Rui WangFlow cytometry analysis Tumors were dissociated into single-cell suspensions using a tumor dissociation kit (130–096-730, Miltenyi Biotec). After red blood cell lysis (ZYFB006-0500, ZUNYAN), viable cells were stained with

(5) Engineered oncolytic virus armed with anti-PCSK9 scFv boosts long-term CD8+ T cell immunity via rewiring MHC-I antigen presentationCell Reports MedicineApril 21, 2026Huolun Feng, Yuhan Zhang, Zuda Huang, Jianlong Zhou, Yongfeng Liu, Xiao Xiao, Mingxi Chen, Xin Guo, Jiabin Zheng, Zejian Lyu, et al.tumor samples underwent harvesting, mincing, and incubation using the Miltenyi Tumor Dissociation Kit (130–096–730). Post-trituration and filtration through a 70-μm screen, cells were resuspended in FACS buffer. A subset

(6) Single-Cell Transcriptomics Reveals Immune Modulation by Telmisartan in Colorectal CancerCellsApril 20, 2026Jinxin Li, Decao Yang, Xiaoyue Wang, Runqing Ju, Shaomeng Chen, Jingyi Zhao, Jiaxing Xu, Jiaxin Chen, Jiayu Ye, Baohui Xu, et al.were harvested and isolated into single cells using a tumor dissociation kit for mouse tissues (Cat#: 130-096-730, Miltenyi, Bergisch Gladbach, Germany). The resulting single-cell suspension was washed with PBS and

(7) A fully human pan VL9 HLA-E TCRm antibody enables functional dissection of HLA-E biology and checkpoint signalingiScienceApril 9, 2026Soroush Ghaffari, Katherine Upchurch-Ange, Gizem Oter, Trivendra Tripathi, Susanne Gimlin, Debra Wawro Weidanz, Jim Middelburg, Thorbald van Hall, Jon A. Weidanzantibody azido modification kit,Thermo Fisher,Cat#S20026 "Tumor Dissociation Kit, mouse",Milteny Biotec,130-096-730 Ventana Discovery Amp HQ kit,Roche,Cat#760-052 Ventana Discovery OmniMap kit,Roche,Cat#760-159 Zombie Aqua Fixable Viability Kit

(8) Hypoalbuminemia induces immunosuppression through disorder in macrophages and drives resistance to immune checkpoint inhibitorsCancer Immunol ImmunotherApril 6, 2026Fan Yang, Yaping Long, Yao Li, Yibing Bai, Yining Liu, Dingyuan Cheng, Tao Li, Yiheng Lu, Xiao Han, Yi HuFlow cytometry Tumors were digested into single-cell suspensions using a Tumor Dissociation Kit (Miltenyi Biotec, 130–096–730). Red blood cells were lysed, and the remaining cells were washed with PBS. For T cell function analysis,

(9) Development and preclinical evaluation of KDTV001, an adenovirus 5-vectored trivalent HPV therapeutic vaccine targeting HPV16/18/52eBioMedicineApril 1, 2026Yilin Dai, Fanwei Huang, Xiaojiao Li, Wenqing Wu, Ruoyu Duan, Xi Li, Xiaoyuan Huang, Qinglei Gao, Ding Ma, Rui Wei, et al.fragments were then washed once with ice bath PBS. The Tumour Tissue Dissociation Kit (Miltenyi Biotec, 130-096-730) was used to digest the tumour tissue fragments for 40 min at 100 rpm in a constant temperature shaker

(10) Robust CD8+ T cell responses induced by an mRNA-LNP vaccine encoding rat HER2 extracellular domain confer prophylactic tumor protectionFront ImmunolApril 1, 2026Kexin Li, Xiaoya Li, Cong Zhang, Suli Dai, Lei Li, Jun Wang, Hongtao Zhang, Lianmei ZhaoTumor specimens were minced and subjected to enzymatic digestion using the Tumor Dissociation Kit (#130-096-730, Miltenyi Biotec, Germany) in a collagenase solution at 37 °C for 40 minutes, followed by filtration

(11) Pharmacologic targeting of the dopamine D2 receptor impacts the efficacy of immune checkpoint blockade in melanomaJ Immunother CancerMarch 27, 2026Mark Gregory, Justin B Hackett, Savannah Hughes, Maria C Muñiz, Samantha Ayoub, Alexander Boerner, Habib Alrabaa, Lucien Taylor, Brianna Rivera, Hanna Holland, et al.performed using Metascape.20 Single-cell RNA sequencing B16F0 tumors were dissociated (Miltenyi, cat. #130-096-730), run through a gradient of 40% and 80% Percoll PLUS (Cytiva, cat. #17544501), and CD45+ enriched (Miltenyi,

(12) Pharmaco-genomic characterization of pancreatic and biliary tract cancer tumoroids for drug responseiScienceMarch 20, 2026Kun Fang, Wenxin Zhang, Qing Zhao, Yan Hong, Ruiqi Wang, Biyu Yang, Yangrong Zhao, Yunkai Shi, Bing Zhou, Jian Ding, et al.assays,Critical commercial assays,Critical commercial assays ,, "Tumor Dissociation Kit, mouse",Miltenyi,Cat# 130-096-730 QlAamp DNA Mini Kit,QIAGEN,Cat# 56304 RNeasy MinElute Cleanup kit,QIAGEN,Cat# 74204 EZ-press RNA Purification Kit

(13) RIPK1 as a potential target to augment DC efficacy in tumor immunotherapyCancer Immunol ImmunotherMarch 17, 2026Lin Mao, Yangjing Ou, Yuchao Zhang, Minghui Wang, Guoqing Dong, Asma Khanniche, Hailong Wang, Zhenchuan Wu, Haibing Zhang, Landian Hu, et al.protocols. Tumor tissue dissociation and cell suspension preparation Tissues were digested (Miltenyi/MACS, 130–096-730), filtered (70 μm MACS SmartStrainer), lysed (ThermoFisher, A1049201), washed with PBS, and resuspended

(14) Disrupting USP14-mediated PARP1 dynamics reinstates MIC-A/B-driven antigen-independent CD8+ T cell killing in gliomaSci AdvMarch 13, 2026Minjie Wang, Shaojie Yu, Chaocai Zhang, Qihong Cheng, Zihan Gong, Xudong Li, Tianzhi Huang, Xuan Wang, Xiaobing Jiang, Junjun LiTumor tissues were dissociated into single-cell suspensions using the Mouse Tumor Dissociation Kit (#130-096-730, Miltenyi Biotec) and filtered through a 200-mesh strainer. Cells were resuspended in RPMI-1640, centrifuged

(15) Ageing promotes metastasis via activation of the integrated stress responseNatureMarch 11, 2026Angana A. H. Patel, Jozefina J. Dzanan, Kevin X. Ali, Ella A. Eklund, Samantha W. Alvarez, Dorota Raj, Martin Dankis, Ilayda Altinönder, Maria Schwarz, Kristell Le Gal, et al.cultures referred to as KP-Y and KP-O were isolated with the tumour dissociation kit for mouse (no. 130-096-730, MACS Miltenyi Biotec) and the gentleMACS Octo Dissociator (no. 130-093-235, MACS Miltenyi Biotec).

(16) Oncolytic virus M1 reinvigorates CD8+ T-cell immunity against glioblastoma through B-cell-dependent antigen cross-presentation in the spleenCell Mol ImmunolMarch 4, 2026Yu Han, Cui Guo, Chaoxin Chen, Wenzhuo Yang, Honghui Li, Changjia He, Jialin Deng, Zhijie Chen, Wenfeng Liu, Jiehong Chen, et al.single-cell suspensions from tumor tissues were prepared via the Tumor Dissociation Kit (Miltenyi Biotec, 130-096-730) according to the manufacturer’s protocol. Splenocytes were isolated as previously described, and single-cell

(17) ATR Blockade Potentiates the Effects of Genotoxic Agents In Vitro and Promotes Antitumor Immunity in a Mouse Model of Non-Small Cell Lung CancerCancers (Basel)March 3, 2026Dimitra Mavroeidi, Christina Papanikolaou, Elisavet Deligianni, Panagiotis Malamos, Panagiota Stamou, Konstantinos N. Syrigos, Vassilis L. Souliotiswith the Mouse Tumor Dissociation Kit from Miltenyi Biotec (Bergisch Gladbach, Rhineland, Germany, #130-096-730,) according to the manufacturer’s instructions. Spleens were harvested and mechanically homogenized

(18) Structurally-optimised HPV16 E7/E6 mRNA–LPP mediates dose-sparing efficacy via tumour microenvironment reprogrammingMolecular BiomedicineFebruary 28, 2026Shucai Sun, Yao Deng, Jiao Ren, Xiaotian Han, Jialuo Bing, Tangqi Wang, Zhanyihao Hao, Houwen Tian, Liang Zhang, Wenjie Tanspecimens underwent enzymatic and mechanical disaggregation using a Miltenyi Tumour Dissociation Kit (cat. 130–096–730) in conjunction with a gentleMACS™ dissociator following the manufacturer’s optimised protocol to generate

(19) TGF ‐β Inhibitor Potentiates Osimertinib‐Induced Anti‐Tumor Immunity in Egfr ‐Mutant Lung CancerCancer ScienceFebruary 27, 2026Tadahiro Kuribayashi, Jun Nishimura, Sachi Okawa, Masataka Taoka, Shunta Mori, Takaaki Tanaka, Tomoka Nishimura, Ayako Morita, Naofumi Hara, Kiichiro Ninomiya, et al.single‐cell suspensions using the Tumor Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany; 130–096‐730); subsequently, red blood cells (RBCs) were removed using Red Blood Cell Lysis Solution (Miltenyi Biotec;

(20) Mesothelioma location influences the tumour microenvironment and immune checkpoint therapy response in preclinical modelsScientific ReportsFebruary 25, 2026M. Lizeth Orozco Morales, Sally M. Lansley, Wee Loong Chin, Caitlin M. Tilsed, Nicola Principe, Connull Leslie, Breana J. Vitali, Francois X. Rwandamuriye, Emma de Jong, Joel Kidman, et al.pieces with a scalpel blade and dissociated with the tumour dissociation kit, mouse (Miltenyi Biotec, 130-096-730) using the hard tumour program in the gentleMACS dissociator (Miltenyi Biotec, 130-093-235). Dissociated

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1/7/2021Mouse Tumor Dissociation Kit Is Useful for Generating Single Cells Ab GMouse cell lines were implanted in mice to generate tumors which were dissociated with the kit to yield single cells.

Tumor Dissociation Kit, mouse (for 50 preparations)

Tumor Dissociation Kit, mouse from Miltenyi Biotec

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Miltenyi Biotec

Miltenyi Biotec

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Tumor Dissociation Kit, mouse from Miltenyi Biotec

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