CellTiter-Glo One Solution Assay G8461 from Promega

Product Specs
ItemCellTiter-Glo One Solution Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG8461
Quantity100ml
Molecular Weight~66kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Quantitative high-throughput screening identifies small-molecule modulators of Wnt/β-catenin signalingFront PharmacolApril 2, 2026Xing Chen, Shu Yang, Srilatha Sakamuru, Chainarong Sukhawanit, Savannah Wood, Masato Ooka, Ruili Huang, Menghang Xiacytotoxicity, cell viability was assessed using the CellTiter-Glo® One Solution Assay reagent (Promega, Cat #G8462). Following the LEF/TCF-bla assay readout, 4 µL of CellTiter-Glo was added directly to each well. Plates

(2) RPA exhaustion activates SLFN11 to eliminate cells with heightened replication stressNature Cell BiologyJanuary 9, 2026Tyler H. Stanage, Shudong Li, Sandra Segura-Bayona, Aurora I. Idilli, Rhona Millar, Graeme Hewitt, Simon J. Boultonμl of complete medium: 90 μl of Cell Titer Glo One Solution Assay reagent (Promega, catalogue number G8462). The 96-well plates were agitated for 5 min at 120 rpm, after which the luminescence readings were

(3) Macropinocytic Uptake and pH‐Responsive Endolysosomal Processing Drive Sustained Chemotherapeutic Efficacy of High‐Load Core@Shell Nanocarriers in Colorectal CancerSmall ScienceJanuary 1, 2026Dolma Choezom, Silke Notter, Titus Griebel, Nathalia Ferreira, Johann Gruetz, Ajinkya Kulkarni, Matthias Schröter, Gražvydas Lukinavičius, Wiebke Möbius, Lena‐Christin Conradi, et al.request. CellTiter‐Glo Viability Assay Cell viability was measured by performing a CellTiter‐Glo assay (Promega, G8461). Cells were seeded in a 96‐well plate. After drug treatment for the indicated duration, 80 μL of the

(4) ENPP1 inhibitor with ultralong drug-target residence time as an innate immune checkpoint blockade cancer therapyCell Reports MedicineSeptember 16, 2025Songnan Wang, Randolph M. Johnson, Jacqueline A. Carozza, Daniel Fernandez, Jan Scicinski, Neil A. Verity, Rachel Mardjuki, Xujun Cao, Yingjie Guo, Jacqueline Papkoff, et al.assays ,, Pierce™ 660nm protein assay reagent,ThermoFisher,Cat# 22660 CellTiterGlo,Promega,Cat# G8461 Pyrophosphate assay kit,Sigma-Aldrich,Cat# MAK168 2′3′-cGAMP ELISA kit,Cayman,Cat# 501700 "Mouse IFN gamma ELISA kit, extra sensitive

(5) The NUDIX hydrolase NUDT5 regulates thiopurine metabolism and cytotoxicityJ Clin InvestJuly 15, 2025Maud Maillard, Rina Nishii, Hieu S. Vu, Kashi R. Bhattarai, Wenjian Yang, Jing Li, Ute Hofmann, Daniel Savic, Smita Bhatia, Matthias Schwab, et al.microwells. After 72 hours of incubation, cell viability was measured by the CellTiter-Glo assay (Promega, G8461), and luminescence was detected with the Synergy H4 hybrid reader (Agilent BioTek). Western blotting. A

(6) AP1-mediated reprogramming of EGFR expression triggers resistance to BLU-667 and LOXO-292 in RET-rearranged tumorsJ Exp Clin Cancer ResMay 22, 2025Daniela Esposito, Claudia Maria Ascione, Stefania Belli, Fabiana Napolitano, Alberto Servetto, Felice Pepe, Umberto Malapelle, Antonino Iaccarino, Giancarlo Troncone, Diletta Barone, et al.μM, threefold dilution). Six days later, a CellTiter-Glo Luminescent Cell Viability Assay (Promega; #G8461) was performed. An equal volume of CellTiter-Glo reagent was added to the culture medium in each well.

(7) Tumor Suppressors Condition Differential Responses to the Selective CDK2 Inhibitor BLU-222Cancer ResearchApril 3, 2025Adam P. Dommer, Vishnu Kumarasamy, Jianxin Wang, Thomas N. O’Connor, Michelle Roti, Sidney Mahan, Karen McLean, Erik S. Knudsen, Agnieszka K. Witkiewiczincubated for five days with drug, at which point 30 μL Cell Titer Glo one solution reagent (Promega, G8461) was added per well. Plates were kept in the dark and shaken briefly before analyzing with a Biotek

(8) Serpina3k lactylation protects from cardiac ischemia reperfusion injuryNat CommunJanuary 25, 2025Le Wang, Dandan Li, Fang Yao, Shanshan Feng, Chao Tong, Rongjia Rao, Meiyan Zhong, Xianqiang Wang, Wei Feng, Zhan Hu, et al.ATP concentrations were assessed using the CellTiter-Glo® Luminescent Cell Viability Assay (Promega, G8461) per manufacturer’s instructions. In brief, 1 µM ATP (Sigma, P1132) diluted with culture medium into

(9) Flap endonuclease 1 repairs DNA-protein cross-links via ADP-ribosylation-dependent mechanismsScience advancesJanuary 10, 2025Sun Y, Jenkins LM, El Touny LH, Zhu L, Yang X, Jo U, Escobedo L, Maity TK, Saha LK, Uribe I, et al.additional 3 days. On day 4, the cell numbers were evaluated using CellTiter-Glo One luminescence (Promega, G8462) read on a BMG Pherastar FSX plate reader. Screen performance was evaluated using AllStars Hs cell death

(10) Regulation of Age-Related Lipid Metabolism in Ovarian CancerInt J Mol SciJanuary 1, 2025Jihua Feng, Clay Douglas Rouse, Lila Taylor, Santiago Garcia, Ethan Nguyen, Isabella Coogan, Olivia Byrd, Andrew Berchuck, Susan K. Murphy, Zhiqing Huang3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay (MTS assay, Cat# G8461, Promega, Durham, NC, USA). The luminescence signals were recorded using a plate reader (BMG Labtech,

(11) Histone deacetylase upregulation of neuropilin-1 in osteosarcoma is essential for pulmonary metastasisCancer LettersDecember 1, 2024Niveditha Nerlakanti, Jeremy J. McGuire, Ryan T. Bishop, Mostafa M. Nasr, Tao Li, Damon R. Reed, Conor C. LynchnM. After 24 and 48 h, 20 μL of CellTiter-Glo® One Solution Assay (Promega, Madison, Wisconsin, Cat#: G8461) was added to wells. Plates were wrapped with aluminum foil and incubated for 10 min at room temperature

(12) Thiophenyl Derivatives of Nicotinamide Are Metabolized by the NAD Salvage Pathway into Unnatural NAD Derivatives That Inhibit IMPDH and Are Toxic to Peripheral Nerve CancersACS chemical biologyJune 21, 2024Theodoropoulos PC, Guo HH, Wang W, Crossley E, Rivera Cancel G, Fang M, Nguyen T, Baniasadi H, Williams NS, Ready JM, et al.cell viability was assayed using an ATP-based luminescent viability assay, CellTiter-Glo (Promega # G8462). The CellTiter-Glo substrate was mixed in a 1:1 ratio with phosphate-buffered saline (PBS) supplemented

(13) Discovery and Optimization of N-Arylated Tetracyclic Dicarboximides That Target Primary Glioma Stem-like CellsJournal of Medicinal ChemistryJune 13, 2024Hua-Yu Wang, Thu P. Nguyen, Alex C. Sternisha, Christopher L. Carroll, Bethany Cross, Lorraine Morlock, Noelle S. Williams, Samuel K. McBrayer, Deepak Nijhawan, Jef K. De BrabanderMut6 or ambient O2 for UTSW63 for 96 h and cell viability was assayed using CellTiter-Glo® (Promega, #G8462) per manufacturer instruction. Data were plotted, and IC50 values were calculated by baseline correction

(14) Targeting CDK9 in Cancer: An Integrated Approach of Combining In Silico Screening with Experimental Validation for Novel DegradersCurrent Issues in Molecular BiologyFebruary 22, 2024Mahesh Koirala, Mario DiPaolawash, a 100 µL volume of 1:1 (V:V) mix of PBS and cell-titer-glo (Promega, Chuo City, Japan, Cat # G8461) was added to each well on each culture plate. Each plate was gently mixed for ca. 10 min at room temperature

(15) Anticancer benzoxaboroles block pre-mRNA processing by directly inhibiting CPSF3Cell Chemical BiologyJanuary 1, 2024Ye Tao, Albert Budhipramono, Ji Huang, Min Fang, Shanhai Xie, Jiwoong Kim, Vishal Khivansara, Zbigniew Dominski, Liang Tong, Jef K. De Brabander, et al.commercial assays,Critical commercial assays,Critical commercial assays CellTiter-Glo,Promega,Cat#G8462 alamarBlue,Bio-Rad,Cat#BUF012B Qiagen RNeasy micro kit,Qiagen,Cat#74004 Deposited data,Deposited

(16) Inducible mismatch repair streamlines forward genetic approaches to target identification of cytotoxic small moleculesCell Chemical BiologyNovember 1, 2023Thu P. Nguyen, Min Fang, Jiwoong Kim, Baiyun Wang, Elisa Lin, Vishal Khivansara, Neha Barrows, Giomar Rivera-Cancel, Maria Goralski, Christopher L. Cervantes, et al.incubated at 37°C, 5% CO2, and 5% O2 for 72h. Cell viability was assayed using CellTiter-Glo® (Promega, #G8462) per manufacturer instruction. Data were plotted, and IC50 values were calculated by baseline correction,

(17) A non-conserved histidine residue on KRAS drives paralog selectivity of the KRASG12D inhibitor MRTX1133Cancer ResearchSeptember 1, 2023Miles A. Keats, John J. W. Han, Yeon-Hwa Lee, Chih-Shia Lee, Ji LuomM. Cell viability assay. Cell viability assay was performed using CellTiter-Glo One reagent (Promega #G8462) following the manufacturer’s instructions. Briefly, human cancer cells (2000 cells per well) or Rasless

(18) ITK degradation to block T cell receptor signaling and overcome therapeutic resistance in T cell lymphomasCell chemical biologyApril 20, 2023Jiang B, Weinstock DM, Donovan KA, Sun HW, Wolfe A, Amaka S, Donaldson NL, Wu G, Jiang Y, Wilcox RA, et al.Protein,R&D,202-IL Critical commercial assays,Critical commercial assays,Critical commercial assays CellTiter-Glo,Promega,#G8462 Pierce BCA Protein Assay Kit,Thermo Scientific,#23225 ECL™ Prime Western Blotting System,Sigma,GERPN2232 Deposited

(19) Inhibiting Type I Arginine Methyltransferase Activity Promotes T Cell-Mediated Antitumor Immune ResponsesCancer immunology researchApril 1, 2022Fedoriw A, Shi L, O'Brien S, Smitheman KN, Wang Y, Hou J, Sherk C, Rajapurkar S, Laraio J, Williams LJ, et al.protocol, and each reporter was normalized to cell growth using CellTiter-Glo (Promega, Madison, WI; G8462) as described in manufacturer’s instructions. VEGF ELISA MC38 cells were seeded in a 6-well plate

(20) Neutral sphingomyelinase 2 controls exosome secretion by counteracting V-ATPase-mediated endosome acidificationJournal of cell scienceMarch 1, 2022Choezom D, Gross JC.Harvard). Viability assay Cell viability was measured by performing a CellTiter-Glo assay (Promega, G8461). Cells were seeded in a 96-well plate. After the indicated treatment, 100 µl of the CellTiter-Glo reagent

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CellTiter-Glo One Solution Assay

CellTiter-Glo One Solution Assay from Promega

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CellTiter-Glo One Solution Assay from Promega

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