CellTiter-Glo One Solution Assay G8461 from Promega

Product Specs
ItemCellTiter-Glo One Solution Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG8461
Quantity100ml
Molecular Weight~66kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(21) Targeting CDK9 in Cancer: An Integrated Approach of Combining In Silico Screening with Experimental Validation for Novel DegradersCurrent Issues in Molecular BiologyFebruary 22, 2024Mahesh Koirala, Mario DiPaolawash, a 100 µL volume of 1:1 (V:V) mix of PBS and cell-titer-glo (Promega, Chuo City, Japan, Cat # G8461) was added to each well on each culture plate. Each plate was gently mixed for ca. 10 min at room temperature

(22) Anticancer benzoxaboroles block pre-mRNA processing by directly inhibiting CPSF3Cell Chemical BiologyJanuary 1, 2024Ye Tao, Albert Budhipramono, Ji Huang, Min Fang, Shanhai Xie, Jiwoong Kim, Vishal Khivansara, Zbigniew Dominski, Liang Tong, Jef K. De Brabander, et al.commercial assays,Critical commercial assays,Critical commercial assays CellTiter-Glo,Promega,Cat#G8462 alamarBlue,Bio-Rad,Cat#BUF012B Qiagen RNeasy micro kit,Qiagen,Cat#74004 Deposited data,Deposited

(23) Inducible mismatch repair streamlines forward genetic approaches to target identification of cytotoxic small moleculesCell Chemical BiologyNovember 1, 2023Thu P. Nguyen, Min Fang, Jiwoong Kim, Baiyun Wang, Elisa Lin, Vishal Khivansara, Neha Barrows, Giomar Rivera-Cancel, Maria Goralski, Christopher L. Cervantes, et al.incubated at 37°C, 5% CO2, and 5% O2 for 72h. Cell viability was assayed using CellTiter-Glo® (Promega, #G8462) per manufacturer instruction. Data were plotted, and IC50 values were calculated by baseline correction,

(24) A non-conserved histidine residue on KRAS drives paralog selectivity of the KRASG12D inhibitor MRTX1133Cancer ResearchSeptember 1, 2023Miles A. Keats, John J. W. Han, Yeon-Hwa Lee, Chih-Shia Lee, Ji LuomM. Cell viability assay. Cell viability assay was performed using CellTiter-Glo One reagent (Promega #G8462) following the manufacturer’s instructions. Briefly, human cancer cells (2000 cells per well) or Rasless

(25) A non-conserved histidine residue on KRAS drives paralog selectivity of the KRASG12D inhibitor MRTX1133Cancer ResSeptember 1, 2023Miles A. Keats, John J. W. Han, Yeon-Hwa Lee, Chih-Shia Lee, Ji LuomM. Cell viability assay. Cell viability assay was performed using CellTiter-Glo One reagent (Promega #G8462) following the manufacturer’s instructions. Briefly, human cancer cells (2000 cells per well) or Rasless

(26) Inhibiting Type I Arginine Methyltransferase Activity Promotes T Cell-Mediated Antitumor Immune ResponsesCancer immunology researchApril 1, 2022Fedoriw A, Shi L, O'Brien S, Smitheman KN, Wang Y, Hou J, Sherk C, Rajapurkar S, Laraio J, Williams LJ, et al.protocol, and each reporter was normalized to cell growth using CellTiter-Glo (Promega, Madison, WI; G8462) as described in manufacturer’s instructions. VEGF ELISA MC38 cells were seeded in a 6-well plate

(27) The impact of SBF2 on taxane-induced peripheral neuropathyPLOS GeneticsJanuary 5, 2022Geneva M. Cunningham et al.post-siRNA delivery (Day 34) by measuring ATP using the CellTiter-Glo One Solution Assay (Promega, #G8461). MACS-sorted cells were treated with paclitaxel 48 hours prior to the viability assessment. Luminescence

(28) Human Pluripotent Stem Cells for High-Throughput Drug Screening and Characterization of Small MoleculesMethods in molecular biology (Clifton, N.J.)January 1, 2022Ryu S, Chu PH, Malley C, Braisted J, Ormanoglu P, Huang R, Itkin M, Itkin Z, Shinn P, Klumpp-Thomas C, et al.cycles. Store m-MPI at −30 °C and buffer at 4 °C.CellTiter-Glo® One Solution Assay (Promega, cat. no. G8461). Thaw and aliquot the solution once received to avoid multiple freeze/thaw cycles. Store at −30 °C.Bovine

(29) The acylfulvene alkylating agent, LP-184, retains nanomolar potency in non-small cell lung cancer carrying otherwise therapy-refractory mutationsOncotargetApril 13, 2021Aditya Kulkarni, Joseph Ryan McDermott, Umesh Kathad, Rama Modali, Jean-Philippe Richard, Panna Sharma, Kishor Bhatia100% signal. Viability of cells was quantified by the CellTiter-Glo® cell viability assay (Promega G8462). After incubation of cells, the CellTiter-Glo® One Solution Assay reagent was brought to ambient temperature.

(30) Ionizing Radiation-induced Proteomic Oxidation in Escherichia coliMolecular & Cellular ProteomicsNovember 23, 2020Steven T. Bruckbauer, Benjamin B. Minkoff, Deyang Yu, Vincent L. Cryns, Michael M. Cox, Michael R. Sussmanduplicate in 96-well plate to assess survival using CellTiter-Glo® assay (Promega; Madison, WI; Cat #: G8461). CellTiter-Glo® assay was performed according the manufacturer's instruction following one, four, and

(31) Ionizing Radiation-induced Proteomic Oxidation in Escherichia coliMol Cell ProteomicsNovember 23, 2020Steven T. Bruckbauer, Benjamin B. Minkoff, Deyang Yu, Vincent L. Cryns, Michael M. Cox, Michael R. Sussmanduplicate in 96-well plate to assess survival using CellTiter-Glo® assay (Promega; Madison, WI; Cat #: G8461). CellTiter-Glo® assay was performed according the manufacturer's instruction following one, four, and

(32) LRRC8A:C/E Heteromeric Channels Are Ubiquitous Transporters of cGAMPMolecular cellNovember 19, 2020Lahey LJ, Mardjuki RE, Wen X, Hess GT, Ritchie C, Carozza JA, Böhnert V, Maduke M, Bassik MC, Li L.Assays NextSeq 500/550 mid output v2 kit,Illumina,Cat#FC-404-2001 CellTiter-Glo One Solution Assay,Promega,Cat#G8461 Deposited Data,Deposited Data,Deposited Data Uncropped Western blots,This paper; Mendeley data,http://dx.doi.org/10.17632/g7rwfvgjbm.1 Experimental Models

(33) Structure-Aided Development of Small-Molecule Inhibitors of ENPP1, the Extracellular Phosphodiesterase of the Immunotransmitter cGAMPCell Chemical BiologyNovember 1, 2020Jacqueline A. Carozza, Jenifer A. Brown, Volker Böhnert, Daniel Fernandez, Yasmeen AlSaif, Rachel E. Mardjuki, Mark Smith, Lingyin Lipaper,N/A Critical Commercial Assays,Critical Commercial Assays,Critical Commercial Assays CellTiterGlo,Promega,Cat#G8461 Deposited Data,Deposited Data,Deposited Data ENPP1-compound 15 structure,This paper,PDB: 6XKD ENPP1-AMP

(34) Interaction between the autophagy protein Beclin 1 and Na+,K+-ATPase during starvation, exercise, and ischemiaJCI insightJanuary 16, 2020Fernández ÁF, Liu Y, Ginet V, Shi M, Nah J, Zou Z, Zhou A, Posner BA, Xiao G, Tanguy M, et al.μL acidified OptiMEM (pH 6.8) for 4 hours and then lysed in 40 μL CellTiter-Glo solution (Promega, G8462). Cellular ATP levels were measured by an EnVision multimode plate reader (PerkinElmer, Inc.). Wells

(35) Multiplexed CRISPR/Cas9 gene knockout with simple crRNA:tracrRNA co-transfectionCell & BioscienceDecember 1, 2019Fehad J. Khan, Garmen Yuen, Ji Luoviability was determined by using the ATP-based CellTiter-Glo Luminescent Cell Viability Assay (Promega #G8462) at day 5 post-transfection following the manufacture’s protocol. Flow cytometry, Western blot, and

(36) The Glucocorticoid Receptor (GR) Stimulates Herpes Simplex Virus 1 Productive Infection, in Part Because the Infected Cell Protein 0 (ICP0) Promoter Is Cooperatively Transactivated by the GR and Krüppel-Like Transcription Factor 15Journal of VirologyMarch 15, 2019Jeffery B. Ostler, Kelly S. Harrison, Kayla Schroeder, Prasanth Thunuguntla, Clinton Jonesin 24-well plates. Viability was assessed using the CellTiterGlo luminescent cell viability assay (G8461; Promega, Madison, WI) according to the manufacturer’s instructions. Plates were read using a SpectraMax M3 microplate reader

(37) Liver-Targeted Anti-HBV Single-Stranded Oligonucleotides with Locked Nucleic Acid Potently Reduce HBV Gene Expression In VivoMolecular therapy. Nucleic acidsJune 1, 2018Javanbakht H, Mueller H, Walther J, Zhou X, Lopez A, Pattupara T, Blaising J, Pedersen L, Albæk N, Jackerott M, et al.cell viability was tested by adding 80 μl/well CellTiter-Glo One Solution Assay Reagent (Promega; no. G8462) to the cells, shaking the plate at RT for 10 min, and then reading the luminescence on an Envision

(38) Targeting Multiple Effector Pathways in Pancreatic Ductal Adenocarcinoma with a G-Quadruplex-Binding Small MoleculeJournal of medicinal chemistryMarch 22, 2018Marchetti C, Zyner KG, Ohnmacht SA, Robson M, Haider SM, Morton JP, Marsico G, Vo T, Laughlin-Toth S, Ahmed AA, et al.then incubated with 100 μL/well of CellTiter-Glo One Solution Assay reagent (Promega, catalogue no. G8461) for 30 min at RT, and luminescence was measured using a PHERAstar FS microplate reader (BMG LabTech).

(39) CRISPR/Cas9-mediated gene knockout is insensitive to target copy number but is dependent on guide RNA potency and Cas9/sgRNA threshold expression levelNucleic Acids ResearchNovember 16, 2017Garmen Yuen, Fehad J. Khan, Shaojian Gao, Jayne M. Stommel, Eric Batchelor, Xiaolin Wu, Ji Luovector, blasticidin Cas9 expression vector). Cell viability was measured using CellTiter-Glo (Promega #G8462). Virus titer was calculated based on the percentage of viable cells and the total number of cells at

(40) Binding of galectin-1 to breast cancer cells MCF7 induces apoptosis and inhibition of proliferation in vitro in a 2D- and 3D- cell culture modelBMC CancerDecember 1, 2016Pamina Geiger, Barbara Mayer, Irmi Wiest, Sandra Schulze, Udo Jeschke, Tobias WeissenbacherTreatment efficacy was assessed using an ATP assay (CellTiter-Glo® Luminescence Cell Viability Assay, G8461, Promega, Germany) to quantify cell survival in vitro. Mean cell survival was expressed as percent of

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CellTiter-Glo One Solution Assay

CellTiter-Glo One Solution Assay from Promega

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CellTiter-Glo One Solution Assay from Promega

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