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CellTiter-Glo One Solution Assay
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CellTiter-Glo One Solution Assay
CellTiter-Glo One Solution Assay
CellTiter-Glo One Solution Assay from Promega
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Citations:
(44)
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CellTiter-Glo One Solution Assay
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Description
The CellTiter-Glo One Solution Assay is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, which indicates the presence of metabolically active cells
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
CellTiter-Glo One Solution Assay
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
G8461
Quantity
100ml
Molecular Weight
~66kDa
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (44)
(21)
A non-conserved histidine residue on KRAS drives paralog selectivity of the KRASG12D inhibitor MRTX1133
Cancer Research
September 1, 2023
Miles A. Keats, John J. W. Han, Yeon-Hwa Lee, Chih-Shia Lee, Ji Luo
mM.
Cell viability assay
.
Cell viability assay
was performed using CellTiter-Glo One reagent (
Promega
#
G8462
) following the manufacturer’s instructions. Briefly,
human
cancer
cells (2000 cells per well) or Rasless
(22)
ITK degradation to block T cell receptor signaling and overcome therapeutic resistance in T cell lymphomas
Cell chemical biology
April 20, 2023
Jiang B, Weinstock DM, Donovan KA, Sun HW, Wolfe A, Amaka S, Donaldson NL, Wu G, Jiang Y, Wilcox RA,
et al
.
Protein,R&D,202-IL Critical commercial
assays
,Critical commercial
assays
,Critical commercial
assays
CellTiter-Glo,
Promega
,#
G8462
Pierce BCA Protein Assay Kit
,
Thermo Scientific
,#
23225
ECL™ Prime
Western Blotting
System,Sigma,GERPN2232 Deposited
(23)
Inhibiting Type I Arginine Methyltransferase Activity Promotes T Cell-Mediated Antitumor Immune Responses
Cancer immunology research
April 1, 2022
Fedoriw A, Shi L, O'Brien S, Smitheman KN, Wang Y, Hou J, Sherk C, Rajapurkar S, Laraio J, Williams LJ,
et al
.
protocol, and each reporter was normalized to cell growth using CellTiter-Glo (
Promega
, Madison, WI;
G8462
) as described in manufacturer’s instructions. VEGF ELISA MC38 cells were seeded in a 6-well plate
(24)
Neutral sphingomyelinase 2 controls exosome secretion by counteracting V-ATPase-mediated endosome acidification
Journal of cell science
March 1, 2022
Choezom D, Gross JC.
Harvard). Viability
assay
Cell viability was measured by performing a CellTiter-Glo
assay
(
Promega
,
G8461
). Cells were seeded in a 96-well plate. After the indicated treatment, 100 µl of the CellTiter-Glo reagent
(25)
The impact of SBF2 on taxane-induced peripheral neuropathy
PLOS Genetics
January 5, 2022
Geneva M. Cunningham et al.
post-siRNA delivery (Day 34) by measuring ATP using the
CellTiter-Glo One Solution Assay
(
Promega
, #
G8461
).
MACS
-sorted cells were treated with paclitaxel 48 hours prior to the viability assessment.
Luminescence
(26)
Human Pluripotent Stem Cells for High-Throughput Drug Screening and Characterization of Small Molecules
Methods in molecular biology (Clifton, N.J.)
January 1, 2022
Ryu S, Chu PH, Malley C, Braisted J, Ormanoglu P, Huang R, Itkin M, Itkin Z, Shinn P, Klumpp-Thomas C,
et al
.
cycles. Store m-MPI at −30 °C and buffer at 4 °C.
CellTiter-Glo® One Solution Assay
(
Promega
, cat. no.
G8461
). Thaw and aliquot the solution once received to avoid multiple freeze/thaw cycles. Store at −30 °C.Bovine
(27)
Belantamab Mafodotin (GSK2857916) Drives Immunogenic Cell Death and Immune-mediated Antitumor Responses
In Vivo
Molecular cancer therapeutics
October 1, 2021
Montes de Oca R, Alavi AS, Vitali N, Bhattacharya S, Blackwell C, Patel K, Seestaller-Wehr L, Kaczynski H, Shi H, Dobrzynski E,
et al
.
for 3 days. Cell growth was evaluated on days 0 and 3 by CellTiter-Glo reagent (
Promega
; catalog No.
G8462
) and
luminescence
analysis. Dose–response curves,
IC50
values and
sensitivity
between cell lines [%
(28)
Anti-tumor effects of an ID antagonist with no observed acquired resistance
NPJ breast cancer
May 24, 2021
Wojnarowicz PM, Escolano MG, Huang YH, Desai B, Chin Y, Shah R, Xu S, Yadav S, Yaklichkin S, Ouerfelli O,
et al
.
AGX51, 1 μM erastin + 1 μM ferrostatin, and 40 μM AGX51 + 1 μM
ferrostatin-1
. Celltiter-Glo (
Promega
G8461
) was used to determine cell viability using the manufacturer’s instructions. Ferroptosis was observed
(29)
The acylfulvene alkylating agent, LP-184, retains nanomolar potency in non-small cell lung cancer carrying otherwise therapy-refractory mutations
Oncotarget
April 13, 2021
Aditya Kulkarni, Joseph Ryan McDermott, Umesh Kathad, Rama Modali, Jean-Philippe Richard, Panna Sharma, Kishor Bhatia
100% signal. Viability of cells was quantified by the CellTiter-Glo®
cell viability assay
(
Promega
G8462
). After
incubation
of cells, the
CellTiter-Glo® One Solution Assay
reagent was brought to ambient temperature.
(30)
Defective ALC1 nucleosome remodeling confers PARPi sensitization and synthetic lethality with HRD
Molecular cell
February 18, 2021
Hewitt G, Borel V, Segura-Bayona S, Takaki T, Ruis P, Bellelli R, Lehmann LC, Sommerova L, Vancevska A, Tomas-Loba A,
et al
.
Commercial
Assays
,Critical Commercial
Assays
,Critical Commercial
Assays
,, CellTiter-Glo,
Promega
,Cat#
G8462
,, "Chemicals, Peptides, and Recombinant Proteins","Chemicals, Peptides, and Recombinant Proteins","Chemicals,
(31)
Ionizing Radiation-induced Proteomic Oxidation in Escherichia coli
Molecular & Cellular Proteomics
November 23, 2020
Steven T. Bruckbauer, Benjamin B. Minkoff, Deyang Yu, Vincent L. Cryns, Michael M. Cox, Michael R. Sussman
duplicate in 96-well plate to assess survival using CellTiter-Glo®
assay
(
Promega
; Madison, WI; Cat #:
G8461
). CellTiter-Glo®
assay
was performed according the manufacturer's instruction following one, four, and
(32)
Ionizing Radiation-induced Proteomic Oxidation in Escherichia coli
Mol Cell Proteomics
November 23, 2020
Steven T. Bruckbauer, Benjamin B. Minkoff, Deyang Yu, Vincent L. Cryns, Michael M. Cox, Michael R. Sussman
duplicate in 96-well plate to assess survival using CellTiter-Glo®
assay
(
Promega
; Madison, WI; Cat #:
G8461
). CellTiter-Glo®
assay
was performed according the manufacturer's instruction following one, four, and
(33)
LRRC8A:C/E Heteromeric Channels Are Ubiquitous Transporters of cGAMP
Molecular cell
November 19, 2020
Lahey LJ, Mardjuki RE, Wen X, Hess GT, Ritchie C, Carozza JA, Böhnert V, Maduke M, Bassik MC, Li L.
Assays
NextSeq 500/550 mid output v2 kit,Illumina,Cat#FC-404-2001
CellTiter-Glo One Solution Assay
,
Promega
,Cat#
G8461
Deposited Data,Deposited Data,Deposited Data Uncropped
Western blots
,This paper; Mendeley data,http://dx.doi.org/10.17632/g7rwfvgjbm.1
Experimental Models
(34)
Structure-Aided Development of Small-Molecule Inhibitors of ENPP1, the Extracellular Phosphodiesterase of the Immunotransmitter cGAMP
Cell Chemical Biology
November 1, 2020
Jacqueline A. Carozza, Jenifer A. Brown, Volker Böhnert, Daniel Fernandez, Yasmeen AlSaif, Rachel E. Mardjuki, Mark Smith, Lingyin Li
paper,N/A Critical Commercial
Assays
,Critical Commercial
Assays
,Critical Commercial
Assays
CellTiterGlo,
Promega
,Cat#
G8461
Deposited Data,Deposited Data,Deposited Data ENPP1-compound 15 structure,This paper,PDB: 6XKD ENPP1-AMP
(35)
Interaction between the autophagy protein Beclin 1 and Na+,K+-ATPase during starvation, exercise, and ischemia
JCI insight
January 16, 2020
Fernández ÁF, Liu Y, Ginet V, Shi M, Nah J, Zou Z, Zhou A, Posner BA, Xiao G, Tanguy M,
et al
.
μL acidified OptiMEM (pH 6.8) for 4 hours and then lysed in 40 μL CellTiter-Glo solution (
Promega
,
G8462
). Cellular ATP levels were measured by an EnVision multimode plate reader (PerkinElmer, Inc.). Wells
(36)
Multiplexed CRISPR/Cas9 gene knockout with simple crRNA:tracrRNA co-transfection
Cell & Bioscience
December 1, 2019
Fehad J. Khan, Garmen Yuen, Ji Luo
viability was determined by using the ATP-based
CellTiter-Glo Luminescent Cell Viability Assay
(
Promega
#
G8462
) at day 5 post-
transfection
following the manufacture’s protocol.
Flow cytometry
,
Western blot
, and
(37)
Genetic interactions of G-quadruplexes in humans
eLife
July 9, 2019
Zyner KG, Mulhearn DS, Adhikari S, Martínez Cuesta S, Di Antonio M, Erard N, Hannon GJ, Tannahill D, Balasubramanian S.
PhenDC3 for 96 hr and determining cell death via a CellTitre-Glo One Solution
assay
(
Promega
, cat #
G8461
) according to manufacturer’s protocol. Each serial dilution was replicated four times for two-cell-seeding
(38)
The Glucocorticoid Receptor (GR) Stimulates Herpes Simplex Virus 1 Productive Infection, in Part Because the Infected Cell Protein 0 (ICP0) Promoter Is Cooperatively Transactivated by the GR and Krüppel-Like Transcription Factor 15
Journal of Virology
March 15, 2019
Jeffery B. Ostler, Kelly S. Harrison, Kayla Schroeder, Prasanth Thunuguntla, Clinton Jones
in 24-well plates. Viability was assessed using the CellTiterGlo luminescent
cell viability assay
(
G8461
;
Promega
, Madison, WI) according to the manufacturer’s instructions. Plates were read using a
SpectraMax M3 microplate reader
(39)
Liver-Targeted Anti-HBV Single-Stranded Oligonucleotides with Locked Nucleic Acid Potently Reduce HBV Gene Expression In Vivo
Molecular therapy. Nucleic acids
June 1, 2018
Javanbakht H, Mueller H, Walther J, Zhou X, Lopez A, Pattupara T, Blaising J, Pedersen L, Albæk N, Jackerott M,
et al
.
cell viability was tested by adding 80 μl/well
CellTiter-Glo One Solution Assay
Reagent (
Promega
; no.
G8462
) to the cells, shaking the plate at RT for 10 min, and then reading the
luminescence
on an Envision
(40)
Targeting Multiple Effector Pathways in Pancreatic Ductal Adenocarcinoma with a G-Quadruplex-Binding Small Molecule
Journal of medicinal chemistry
March 22, 2018
Marchetti C, Zyner KG, Ohnmacht SA, Robson M, Haider SM, Morton JP, Marsico G, Vo T, Laughlin-Toth S, Ahmed AA,
et al
.
then incubated with 100 μL/well of
CellTiter-Glo One Solution Assay
reagent (
Promega
, catalogue no.
G8461
) for 30 min at RT, and
luminescence
was measured using a
PHERAstar FS
microplate reader (
BMG LabTech
).
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CellTiter-Glo One Solution Assay from Promega
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