Complex I Enzyme Activity Microplate Assay Kit (Colorimetric) from Abcam

Product Specs
ItemComplex I Enzyme Activity Microplate Assay Kit (Colorimetric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab109721
Quantity96 Test
MethodFuncS
Sample TypeCell culture extracts, Tissue
Time3h 30m
ApplicationsEnzyme activity assay

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Abcam

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Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) S14G‐humanin attenuates renal ischaemia–reperfusion injury, potentially through activation of STAT3 and ERK1/2 signalling pathways in ratsExp PhysiolAugust 31, 2026Leyla Abueid, Ahmet Faruk Torun, Ezgi Golal, Nuray Acar, Filiz Basraliactivity in kidney tissue homogenates was determined using a Complex I Activity Assay Kit (Abcam, cat. no. ab109721), following the manufacturer's protocol. Results were expressed as enzyme activity per milligram of

(2) Ligand-independent EPHA2 signaling sustains neural progenitors via ECSIT and NAD()EMBO reportsAugust 1, 2026Diem Nghi T, My Nhung TT, Kim S, Yun J, Tien NTN, Lee A, Jung M, Jang EJ, Goo BS, Yoo JY, et al.Mitochondria Isolation Kit for Cultured Cells,Thermo Scientific,Cat# 89874 Complex I Enzyme Activity Microplate Assay Kit (Colorimetric),Abcam,Cat# ab109721 MitoXpress Xtra Oxygen Consumption Assay,Agilent Technologies,Cat# MX-200-4 Seahorse XF Mito Stress Test

(3) Mitochondrial metabolism determines chemotherapy sensitivity in colorectal cancerNature MetabolismJuly 23, 2026Deborah Y. Moss, Connor N. Brown, Andrew M. Shaw, Christopher McCann, Nikita Lewis, Matilda Downs, Rebecca Wurelly, Ciara Cunningham, Aaron Philips, Niamh Doherty, et al.activity of CI was determined by a colourimetric analysis following the manufacturer’s protocol (Abcam, ab109721). A Seahorse XFe96 Analyser (Agilent Technologies) was used to assess mitochondrial complex-specific

(4) Plant-derived mitochondria mitigate aging-related neurodegeneration by reprogramming microglial mitochondrial energy metabolismTransl NeurodegenerJuly 8, 2026Yun Teng, Chao Luo, Qingbo Xu, Jingyao Mu, Lucy Teng, Hongjia Qian, Yinan Huang, Minmin Liu, Lifeng Zhang, Juw Won Park, et al.assay Complex I activity was assessed using a Complex I Enzyme Activity Microplate Assay Kit (Abcam, Cat# ab109721, Cambridge, UK) according to the manufacturer’s instructions. Briefly, 2 × 105 cells or 1.0 mg isolated

(5) Targeting Dynamin-Related Protein 1 and Glucose Metabolism Reverses Acquired Resistance to Sorafenib in Liver CancerOncology ResearchJune 16, 2026Jinhui Che, Zhiyuan Chen, Feng Zhang, Shuo Zhu, Shengya Cao, Ou Li, Rong Li, Jun Zheng, Yubin Liucomplex I–V were measured using enzyme assay kits according to the manufacturer’s protocols (Abcam, ab109721, ab109908, ab287844, ab109911 and ab109714). Briefly, fresh mitochondria were isolated using the Mitochondria

(6) AMC-F1 regulates mitochondria-autophagy crosstalk independent of nutrient stressNature CommunicationsJune 5, 2026Yuqin Wang, Raksha K. Rao, Trung Vu, Ayano Sekine, Zhengmei Mao, Nami McCartyactivity Mitochondrial Complex I activity was measured using the Complex I Enzyme Activity Assay Kit (Abcam, ab109721) according to the manufacturer’s instructions. Briefly, adherent cells were scraped from confluent culture

(7) Correction of Hypoxemia and Hypoglycemia Restores Muscle Mitochondrial Respiration and Remodels Mitochondrial Proteome in Growth-Restricted Sheep FetusesAmerican Journal of Physiology-Endocrinology and MetabolismJune 1, 2026Weicheng Zhao, Daniel B. Chrisenberry, Mariangel Varela, Rosa I. Luna-Ramirez, Miranda J. Anderson, Paul R. Langlais, Laura D. Brown, Sean W. Limesand(10) Mitochondrial complex I activity was measured in the same cohort using a colorimetric assay (Abcam, ab109721). Frozen mitochondrial pellets were resuspended in ice-cold PBS with detergent and centrifuged (16,000

(8) Mitochondrial protection role of oxidoreductase-like domain containing 1 in myocardial cells under hypoxiaMedical Gas ResearchJune 1, 2026Yan Yan, Min Dong, Liuyang Tian, Chao Zhu, Xiaojing ZhaoTokyo, Japan, SH800).1617 Detection of mitochondrial complex activity Complex I activity (Abcam, Cat# ab109721) was measured, and Complex II to V activities (Cayman, Cat# 700950, 700990, and 701000) were measured

(9) APOE4 exacerbates glucocorticoid stress hormone-induced tau pathology via mitochondrial dysfunctionCell Death & DiseaseMarch 27, 2026Qing Yu, Fang Du, Veria Puerta-Alvarado, Jeffrey H. Goodman, Clarissa L. WaitesDIV hippocampal neurons or hippocampal tissue with Complex I Enzyme Activity Microplate Assay Kit (ab109721, Abcam) and ATP Assay Kit (Colorimetric/Fluorometric, ab83355, Abcam), respectively, according to the

(10) miR-4516-Loaded Engineered Milk Extracellular Vesicles Attenuate Indoxyl Sulfate-Induced Mitochondrial Dysfunction and Improve Renal Function in a CKD Mouse ModelInternational Journal of Molecular SciencesMarch 25, 2026Jeongkun Lee, Jun Young Yoon, Jae Young Lee, Sang Hun Leeactivity assays. Complex I activity was measured using the Complex I Enzyme Activity Microplate Assay Kit (ab109721; Abcam, Cambridge, UK) according to the manufacturer’s instructions. Complex IV activity was measured

(11) Stroke in persistent chronic kidney disease condition alters innate-immunity to escalate mitochondrial dysfunction and agingnpj AgingMarch 11, 2026Aishika Datta, Karan Sehgal, Deepaneeta Sarmah, Smreeti Dhiman, Birva Shah, Pooja Dhakne, Gautam Karmarkar, Anirban Barik, Anita Kumari, Ushmita Mukherjee, et al.I, II, and IV were evaluated using commercially available kits: Complex I Assay Kit (Abcam; Cat. No. ab109721), Complex II Assay Kit (Abcam; Cat. No. ab109908) and Complex IV Assay Kit (Abacm; Cat. No. ab109906).

(12) Visomitin as a differentiation-inducing therapeutic agent through SYK inhibition in AMLFrontiers in PharmacologyFebruary 24, 2026Byeol-Eun Jeon, Chan-Seong Kwon, Ji-Eun Lee, Su-Ji Lee, Youngseuk Cho, Ho-Jin Shin, Sang-Woo Kim, Youngmi Jungactivity was then measured using the Complex I Enzyme Activity Microplate Assay Kit (Colorimetric; Abcam, ab109721) according to the manufacturer’s instructions. Luminescence was measured at 450 nm using a GloMax™ Microplate

(13) HUWE1 regulates mitophagy to protect dopaminergic neurons from 6-OHDA- and MPP⁺-induced neurotoxicityCell Biol ToxicolFebruary 5, 2026Chanhaeng Lee, Dong Yeol Kim, Sang-Min Kim, Inn-Oc Hanmitochondrial Complex I and Complex IV were measured using the Complex I Enzyme Activity Microplate Assay Kit (ab109721, Abcam, Cambridge, UK) and the Complex IV Human Enzyme Activity Microplate Assay Kit (ab109910, Abcam),

(14) Mitochondrial and lysosomal dysfunctions might be involved in the pathogenesis of the CACNA1A-related neurodevelopmental disorders according to in vitro studiesBiol ResDecember 27, 2025Miriam Kessi, Langui Pan, Baiyu Chen, Li Yang, Lifen Yang, Olumuyiwa A. Bamgbade, Guoli Wang, Jing Peng, Fei Yin, Fang Heinstructions provided in the complex I enzyme activity assay kit (Colorimetric, Abcam, catalog number ab109721, available at http://www.abcam.com/ab109721). The transfected cells were washed twice with phosphate-buffered

(15) Oenological tannins mitigate rotenone-induced mitochondrial impairments and oxidative stress, with concomitant detection of urolithin A in the brainBiochemistry and biophysics reportsDecember 1, 2025Wojciechowska O, Jourdes M, Andrusiewicz M, Pokrzywa M, Karaźniewicz-Łada M, Jodynis-Liebert J, Teissedre PL, Kujawska M.reduction of a dye detected spectrophotometrically at 450 nm using the Abcam Complex I Enzyme Activity Kit (ab109721, Abcam, Amsterdam, NL) per the manufacturer's directions. The Protein Carbonyl (PC) content was quantified

(16) NDUFS3 promotes proliferation via glucose metabolism reprogramming inducing AMPK phosphorylating PRPS1 to increase the purine nucleotide synthesis in melanomaCell Death & DifferentiationDecember 1, 2025Guohang Xiong, Fang Yun, Lu Jiang, Zihan Yi, Xiaojia Yi, Lijuan Yang, Xuedan Zhang, Xiaoyu Li, Zhe Yang, Qiao Zhang, et al.#ab110170). Then, detect the activity of CI according to the instructions of the reagent supplier (Abcam, #ab109721). Finally, measure the content of CI following the instructions from the reagent supplier (Tianjin Yisenyuan

(17) Cancer suppresses mitochondrial chaperone activity in macrophages to drive immune evasionNature ImmunologyDecember 1, 2025Haoxin Zhao, Jaeoh Park, Yuzhu Wang, Yi-Ju Chou, Lemin Li, Lydia N. Raines, Michael Hsu, Ching-Cheng Lin, Wei Cao, Yuli Ouyang, et al.by the Complex I Enzyme Activity Assay Kit (Colorimetric) according to the manufacturer’s protocol (ab109721, Abcam). For Complex II (SDH) activity assay, 2 × 106 macrophages were plated and treated with or without

(18) FGF2 Attenuated Inflammation-Mediated Cardiac Damage: A Novel Mechanistic Insight into the AMPK–FUNDC1–MitophagyResearch (Wash D C)November 24, 2025Pingjun Zhu, Xi Wang, Xinjie Han, Yan Wang, Yongkai Ding, Min Zhu, Xin Zhang, Qian Xu, Yukun Li, Zhongxuan Li, et al.mitochondrial respiratory complex I activities (Mouse Mitochondrial Respiratory Chain Complex I ELISA Kit, ab109721, Abcam), mitochondrial respiratory complex II activities (Mouse Mitochondrial Respiratory Chain Complex

(19) Rotenone targets midbrain astrocytes to produce glial dysfunction-mediated dopaminergic neurodegenerationActa Neuropathologica CommunicationsNovember 14, 2025Ikuko Miyazaki, Nami Isooka, Ryo Kikuoka, Fuminori Imafuku, Kaori Masai, Kana Tomimoto, Masakiyo Sakaguchi, Chiharu Sogawa, Norio Sogawa, Yoshihisa Kitamura, et al.glia, we measured complex I enzyme activity using the Complex I Enzyme Activity Microplate Assay Kit (ab109721, Abcam, Cambridge, UK), according to the manufacturer’s instructions. The detailed experimental procedures

(20) ALKBH1 Drives Tumorigenesis and Drug Resistance via tRNA-decoding Reprogramming and Codon-biased TranslationCancer DiscoveryNovember 3, 2025Chao Shen, Yuan Che, Keren Zhou, Kitty Wang, Wei Li, Diyuan Xue, Tong Wu, Lu Yang, Meiling Chen, Yue Sheng, et al.complex activity Measurements of complex I activity (Complex I Enzyme Activity Microplate Assay Kit, #ab109721, Abcam), complex IV activity (Complex IV Enzyme Activity Microplate Assay Kit, #ab109909, Abcam), and

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Complex I Enzyme Activity Microplate Assay Kit (Colorimetric) from Abcam

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Complex I Enzyme Activity Microplate Assay Kit (Colorimetric) from Abcam

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