Complex I Enzyme Activity Microplate Assay Kit (Colorimetric) ab109721 from Abcam

Product Specs
ItemComplex I Enzyme Activity Microplate Assay Kit (Colorimetric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab109721
Quantity96 Test
MethodFuncS
Sample TypeCell culture extracts, Tissue
Assay Time3h 30m
ApplicationsEnzyme activity assay

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Abcam

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Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Mitochondrial protection role of oxidoreductase-like domain containing 1 in myocardial cells under hypoxiaMedical Gas ResearchJune 1, 2026Yan Yan, Min Dong, Liuyang Tian, Chao Zhu, Xiaojing ZhaoTokyo, Japan, SH800).1617 Detection of mitochondrial complex activity Complex I activity (Abcam, Cat# ab109721) was measured, and Complex II to V activities (Cayman, Cat# 700950, 700990, and 701000) were measured

(2) miR-4516-Loaded Engineered Milk Extracellular Vesicles Attenuate Indoxyl Sulfate-Induced Mitochondrial Dysfunction and Improve Renal Function in a CKD Mouse ModelInternational Journal of Molecular SciencesMarch 25, 2026Jeongkun Lee, Jun Young Yoon, Jae Young Lee, Sang Hun Leeactivity assays. Complex I activity was measured using the Complex I Enzyme Activity Microplate Assay Kit (ab109721; Abcam, Cambridge, UK) according to the manufacturer’s instructions. Complex IV activity was measured

(3) Visomitin as a differentiation-inducing therapeutic agent through SYK inhibition in AMLFrontiers in PharmacologyFebruary 24, 2026Byeol-Eun Jeon, Chan-Seong Kwon, Ji-Eun Lee, Su-Ji Lee, Youngseuk Cho, Ho-Jin Shin, Sang-Woo Kim, Youngmi Jungactivity was then measured using the Complex I Enzyme Activity Microplate Assay Kit (Colorimetric; Abcam, ab109721) according to the manufacturer’s instructions. Luminescence was measured at 450 nm using a GloMax™ Microplate

(4) HUWE1 regulates mitophagy to protect dopaminergic neurons from 6-OHDA- and MPP⁺-induced neurotoxicityCell Biol ToxicolFebruary 5, 2026Chanhaeng Lee, Dong Yeol Kim, Sang-Min Kim, Inn-Oc Hanmitochondrial Complex I and Complex IV were measured using the Complex I Enzyme Activity Microplate Assay Kit (ab109721, Abcam, Cambridge, UK) and the Complex IV Human Enzyme Activity Microplate Assay Kit (ab109910, Abcam),

(5) Mitochondrial and lysosomal dysfunctions might be involved in the pathogenesis of the CACNA1A-related neurodevelopmental disorders according to in vitro studiesBiol ResDecember 27, 2025Miriam Kessi, Langui Pan, Baiyu Chen, Li Yang, Lifen Yang, Olumuyiwa A. Bamgbade, Guoli Wang, Jing Peng, Fei Yin, Fang Heinstructions provided in the complex I enzyme activity assay kit (Colorimetric, Abcam, catalog number ab109721, available at http://www.abcam.com/ab109721). The transfected cells were washed twice with phosphate-buffered

(6) NDUFS3 promotes proliferation via glucose metabolism reprogramming inducing AMPK phosphorylating PRPS1 to increase the purine nucleotide synthesis in melanomaCell Death & DifferentiationDecember 1, 2025Guohang Xiong, Fang Yun, Lu Jiang, Zihan Yi, Xiaojia Yi, Lijuan Yang, Xuedan Zhang, Xiaoyu Li, Zhe Yang, Qiao Zhang, et al.#ab110170). Then, detect the activity of CI according to the instructions of the reagent supplier (Abcam, #ab109721). Finally, measure the content of CI following the instructions from the reagent supplier (Tianjin Yisenyuan

(7) Oenological tannins mitigate rotenone-induced mitochondrial impairments and oxidative stress, with concomitant detection of urolithin A in the brainBiochemistry and Biophysics ReportsDecember 1, 2025Olga Wojciechowska, Michaël Jourdes, Mirosław Andrusiewicz, Małgorzata Pokrzywa, Marta Karaźniewicz-Łada, Jadwiga Jodynis-Liebert, Pierre-Louis Teissedre, Małgorzata Kujawskareduction of a dye detected spectrophotometrically at 450 nm using the Abcam Complex I Enzyme Activity Kit (ab109721, Abcam, Amsterdam, NL) per the manufacturer's directions. The Protein Carbonyl (PC) content was quantified

(8) FGF2 Attenuated Inflammation-Mediated Cardiac Damage: A Novel Mechanistic Insight into the AMPK–FUNDC1–MitophagyResearch (Wash D C)November 24, 2025Pingjun Zhu, Xi Wang, Xinjie Han, Yan Wang, Yongkai Ding, Min Zhu, Xin Zhang, Qian Xu, Yukun Li, Zhongxuan Li, et al.mitochondrial respiratory complex I activities (Mouse Mitochondrial Respiratory Chain Complex I ELISA Kit, ab109721, Abcam), mitochondrial respiratory complex II activities (Mouse Mitochondrial Respiratory Chain Complex

(9) Rotenone targets midbrain astrocytes to produce glial dysfunction-mediated dopaminergic neurodegenerationActa Neuropathologica CommunicationsNovember 14, 2025Ikuko Miyazaki, Nami Isooka, Ryo Kikuoka, Fuminori Imafuku, Kaori Masai, Kana Tomimoto, Masakiyo Sakaguchi, Chiharu Sogawa, Norio Sogawa, Yoshihisa Kitamura, et al.glia, we measured complex I enzyme activity using the Complex I Enzyme Activity Microplate Assay Kit (ab109721, Abcam, Cambridge, UK), according to the manufacturer’s instructions. The detailed experimental procedures

(10) ALKBH1 Drives Tumorigenesis and Drug Resistance via tRNA-decoding Reprogramming and Codon-biased TranslationCancer DiscoveryNovember 3, 2025Chao Shen, Yuan Che, Keren Zhou, Kitty Wang, Wei Li, Diyuan Xue, Tong Wu, Lu Yang, Meiling Chen, Yue Sheng, et al.complex activity Measurements of complex I activity (Complex I Enzyme Activity Microplate Assay Kit, #ab109721, Abcam), complex IV activity (Complex IV Enzyme Activity Microplate Assay Kit, #ab109909, Abcam), and

(11) Development of Two In Vitro ND1-LHON Models for Evaluating Gene Therapy EfficacyInvest Ophthalmol Vis SciOctober 23, 2025Xin Li, Jun Yuan, Zhang Chen, Yue Zhang, Yuefeng Liu, Yong ZhangAssay The complex I activities were measured using a Complex I Enzyme Activity Microplate Assay kit (Abcam; ab109721), following the manufacturer’s instructions. Mitochondrial Respiration Analysis Mitochondrial respiration

(12) Treatment of Mitochondrial Disturbances due to Early Life Adversity in Mice Results in Restoration of Complex I Activity and Normal Reward BehavioreNeuroSeptember 11, 2025Kathie L. Eagleson, Pat Levittimmuno-capture ELISA complex I enzyme activity assay kit, according to the manufacturer's protocol (#ab109721, Abcam). To ensure that all assays were in the linear range, three dilutions of mitochondrial proteins

(13) PKCδ Regulates DNA Damage and Cell Death through a SIRT6/Nrf2-dependent Antioxidant ResponseMolecular Cancer ResearchSeptember 4, 2025Trisiani Affandi, Angela M. Ohm, Jordan T. Speidel, M. Cecilia Caino, Dillon P. Boulton, Mary E. Reylandassay. Complex I activity was measured with the Complex I Enzyme Activity Microplate Assay Kit (Cat # ab109721, Abcam) using equal amounts of total protein (1.25 mg/mL). Complex III activity was assayed using the

(14) Reduced complex I activity in the retinal pigment epithelium, but not in rod photoreceptors, affects light signaling without impacting cell survivalJournal of Biological ChemistrySeptember 1, 2025Alexander M. Warwick, Lin Yu, Mikael Klingeborn, Amanda M. Travis, Vipul M. Parmar, Howard M. Bomze, Goldis Malek, Sidney M. Gospe IIIRPE/choroid lysates was performed using the Complex I Enzyme Activity Assay Kit (colorimetric) (Abcam, ab109721). For iCre75;ndufs4loxP/loxP mice (n = 7) and ndufs4loxP/loxP littermates (n = 5), the retinas were

(15) Dual Regulation of Mitochondrial Complexes by H2S via S-Sulfhydration Controls Respiration in Type 1 Diabetic HeartsBiomoleculesAugust 20, 2025Tong Su, Li Han Zhu, Jun Xian Liu, Li Yuan Jin, Huixing Cui, Longhao Yu, Yin Hua Zhangmitochondrial complexes I-V were determined using enzyme-linked immunosorbent assay (ELISA) kits (Abcam, UK ab109721, ab109928, ab287844, ab109911, ab109714). The experiments were performed according to the manufacturer’

(16) Silencing mitochondrial gene expression in living cellsScienceJuly 31, 2025Luis Daniel Cruz-Zaragoza, Drishan Dahal, Mats Koschel, Angela Boshnakovska, Aiturgan Zheenbekova, Mehmet Yilmaz, Marcel Morgenstern, Jan-Niklas Dohrke, Julian Bender, Anusha Valpadashi, et al.using enzyme activity microplate assay kits from Abcam: Complex I Enzyme Activity Microplate Assay Kit (ab109721), Mitochondrial Complex III Activity Assay Kit (ab287844), Complex IV Rodent Enzyme Activity Microplate Assay Kit

(17) Mesencephalic Astrocyte‐Derived Neurotrophic Factor Binds BAX to Preserve Mitochondrial Homeostasis and Energy Metabolism for Relieving Myocardial HypertrophyAdvanced ScienceJuly 30, 2025Dong Wang, Xinru Zhang, Baolong Wang, Haipeng Li, Dongshuo Xu, Yun Yang, Jialu Zhang, Wenbing Wang, Ren Zhang, Xinyu Wang, et al.examination according to the manufacturer's instructions. Complex I Enzyme Activity Assay Kit (Abcam, ab109721), MitoTox Complex II+III OXPHOS Activity Assay Kit (Abcam, ab109905), and Complex IV Rodent Enzyme Activity Microplate Assay Kit

(18) Mitochondrial dysfunction enhances influenza pathogenesis by up-regulating de novo sialic acid biosynthesisSci AdvJuly 4, 2025Amanda L. Fuchs, Bharati Singh, Jillian W. Jetmore, Emily B. Warren, Payal Banerjee, Jose L. Marin Franco, Michael A. Eckhaus, Tatiana N. Tarasenko, Madeleine R. Assaad, Ivan Kosik, et al.activity was measured using an enzyme assay kit following the manufacturer’s protocol (catalog no. ab109721, Abcam). Briefly, 100 μg of lung tissue was homogenized in cold PBS using a Dounce homogenizer. Protein

(19) Immature Acta2R179C/+ smooth muscle cells cause moyamoya-like cerebrovascular lesions in mice prevented by boosting OXPHOSNat CommunJuly 2, 2025Anita Kaw, Suravi Majumder, Jose E. Esparza Pinelo, Ting Wu, Zbigniew Starosolski, Zhen Zhou, Albert J. Pedroza, Xueyan Duan, Kaveeta Kaw, Angie D. Gonzalez, et al.using the Complex I Enzyme Activity Assay Kit according to the manufacturer’s instructions (Abcam, ab109721). Briefly, mitochondria were isolated (Mitochondria Isolation Kit, ab110168) from vehicle- and NR-treated

(20) Omaveloxolone, But Not Dimethyl Fumarate, Improves Cardiac Function in Friedreich's Ataxia Mice With Severe CardiomyopathyJournal of the American Heart AssociationJune 17, 2025Lili Salinas, Francisco Figueroa, Claire B. Montgomery, Phung N. Thai, Nipavan Chiamvimonvat, Gino Cortopassi, Elena N. DedkovaMitochondrial enzyme activities were determined with Abcam assays for aconitase (ab109712), Complex I (ab109721), and Complex II (ab109908). Differentiated FA Fibroblasts FA skin fibroblasts were obtained from

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Complex I Enzyme Activity Microplate Assay Kit (Colorimetric) from Abcam

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Complex I Enzyme Activity Microplate Assay Kit (Colorimetric) from Abcam

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