Complex I Enzyme Activity Microplate Assay Kit (Colorimetric) ab109721 from Abcam

Product Specs
ItemComplex I Enzyme Activity Microplate Assay Kit (Colorimetric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab109721
Quantity96 Test
MethodFuncS
Sample TypeCell culture extracts, Tissue
Assay Time3h 30m
ApplicationsEnzyme activity assay

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Website: http://www.abcam.com

(21) ALKBH1 drives tumorigenesis and drug resistance via tRNA decoding reprogramming and codon-biased translationCancer DiscovNovember 3, 2025Chao Shen, Yuan Che, Keren Zhou, Kitty Wang, Wei Li, Diyuan Xue, Tong Wu, Lu Yang, Meiling Chen, Yue Sheng, et al.complex activity Measurements of complex I activity (Complex I Enzyme Activity Microplate Assay Kit, #ab109721, Abcam), complex IV activity (Complex IV Enzyme Activity Microplate Assay Kit, #ab109909, Abcam), and

(22) Development of Two In Vitro ND1-LHON Models for Evaluating Gene Therapy EfficacyInvestigative ophthalmology & visual scienceOctober 1, 2025Li X, Yuan J, Chen Z, Zhang Y, Liu Y, Zhang Y.Assay The complex I activities were measured using a Complex I Enzyme Activity Microplate Assay kit (Abcam; ab109721), following the manufacturer’s instructions. Mitochondrial Respiration Analysis Mitochondrial respiration

(23) Treatment of Mitochondrial Disturbances due to Early Life Adversity in Mice Results in Restoration of Complex I Activity and Normal Reward BehavioreNeuroSeptember 11, 2025Kathie L. Eagleson, Pat Levittimmuno-capture ELISA complex I enzyme activity assay kit, according to the manufacturer's protocol (#ab109721, Abcam). To ensure that all assays were in the linear range, three dilutions of mitochondrial proteins

(24) Treatment of Mitochondrial Disturbances due to Early Life Adversity in Mice Results in Restoration of Complex I Activity and Normal Reward BehavioreNeuroSeptember 11, 2025Kathie L. Eagleson, Pat Levittimmuno-capture ELISA complex I enzyme activity assay kit, according to the manufacturer's protocol (#ab109721, Abcam). To ensure that all assays were in the linear range, three dilutions of mitochondrial proteins

(25) PKCδ Regulates DNA Damage and Cell Death through a SIRT6/Nrf2-dependent Antioxidant ResponseMolecular Cancer ResearchSeptember 4, 2025Trisiani Affandi, Angela M. Ohm, Jordan T. Speidel, M. Cecilia Caino, Dillon P. Boulton, Mary E. Reylandassay. Complex I activity was measured with the Complex I Enzyme Activity Microplate Assay Kit (Cat # ab109721, Abcam) using equal amounts of total protein (1.25 mg/mL). Complex III activity was assayed using the

(26) PKCδ regulates DNA damage and cell death through a SIRT6/Nrf2-dependent antioxidant responseMol Cancer ResSeptember 4, 2025Trisiani Affandi, Angela M. Ohm, Jordan T. Speidel, M. Cecilia Caino, Dillon P. Boulton, Mary E. Reylandassay. Complex I activity was measured with the Complex I Enzyme Activity Microplate Assay Kit (Cat # ab109721, Abcam) using equal amounts of total protein (1.25 mg/mL). Complex III activity was assayed using the

(27) Reduced complex I activity in the retinal pigment epithelium, but not in rod photoreceptors, affects light signaling without impacting cell survivalJournal of Biological ChemistrySeptember 1, 2025Alexander M. Warwick, Lin Yu, Mikael Klingeborn, Amanda M. Travis, Vipul M. Parmar, Howard M. Bomze, Goldis Malek, Sidney M. Gospe IIIRPE/choroid lysates was performed using the Complex I Enzyme Activity Assay Kit (colorimetric) (Abcam, ab109721). For iCre75;ndufs4loxP/loxP mice (n = 7) and ndufs4loxP/loxP littermates (n = 5), the retinas were

(28) Dual Regulation of Mitochondrial Complexes by H2S via S-Sulfhydration Controls Respiration in Type 1 Diabetic HeartsBiomoleculesAugust 20, 2025Tong Su, Li Han Zhu, Jun Xian Liu, Li Yuan Jin, Huixing Cui, Longhao Yu, Yin Hua Zhangmitochondrial complexes I-V were determined using enzyme-linked immunosorbent assay (ELISA) kits (Abcam, UK ab109721, ab109928, ab287844, ab109911, ab109714). The experiments were performed according to the manufacturer’

(29) Mitochondrial protection role of oxidoreductase-like domain containing 1 in myocardial cells under hypoxiaMed Gas ResAugust 18, 2025Yan Yan, Min Dong, Liuyang Tian, Chao Zhu, Xiaojing ZhaoTokyo, Japan, SH800).1617 Detection of mitochondrial complex activity Complex I activity (Abcam, Cat# ab109721) was measured, and Complex II to V activities (Cayman, Cat# 700950, 700990, and 701000) were measured

(30) Silencing mitochondrial gene expression in living cellsScienceJuly 31, 2025Luis Daniel Cruz-Zaragoza, Drishan Dahal, Mats Koschel, Angela Boshnakovska, Aiturgan Zheenbekova, Mehmet Yilmaz, Marcel Morgenstern, Jan-Niklas Dohrke, Julian Bender, Anusha Valpadashi, et al.using enzyme activity microplate assay kits from Abcam: Complex I Enzyme Activity Microplate Assay Kit (ab109721), Mitochondrial Complex III Activity Assay Kit (ab287844), Complex IV Rodent Enzyme Activity Microplate Assay Kit

(31) Mesencephalic Astrocyte‐Derived Neurotrophic Factor Binds BAX to Preserve Mitochondrial Homeostasis and Energy Metabolism for Relieving Myocardial HypertrophyAdvanced ScienceJuly 30, 2025Dong Wang, Xinru Zhang, Baolong Wang, Haipeng Li, Dongshuo Xu, Yun Yang, Jialu Zhang, Wenbing Wang, Ren Zhang, Xinyu Wang, et al.examination according to the manufacturer's instructions. Complex I Enzyme Activity Assay Kit (Abcam, ab109721), MitoTox Complex II+III OXPHOS Activity Assay Kit (Abcam, ab109905), and Complex IV Rodent Enzyme Activity Microplate Assay Kit

(32) Mitochondrial dysfunction enhances influenza pathogenesis by up-regulating de novo sialic acid biosynthesisSci AdvJuly 4, 2025Amanda L. Fuchs, Bharati Singh, Jillian W. Jetmore, Emily B. Warren, Payal Banerjee, Jose L. Marin Franco, Michael A. Eckhaus, Tatiana N. Tarasenko, Madeleine R. Assaad, Ivan Kosik, et al.activity was measured using an enzyme assay kit following the manufacturer’s protocol (catalog no. ab109721, Abcam). Briefly, 100 μg of lung tissue was homogenized in cold PBS using a Dounce homogenizer. Protein

(33) Immature Acta2R179C/+ smooth muscle cells cause moyamoya-like cerebrovascular lesions in mice prevented by boosting OXPHOSNat CommunJuly 2, 2025Anita Kaw, Suravi Majumder, Jose E. Esparza Pinelo, Ting Wu, Zbigniew Starosolski, Zhen Zhou, Albert J. Pedroza, Xueyan Duan, Kaveeta Kaw, Angie D. Gonzalez, et al.using the Complex I Enzyme Activity Assay Kit according to the manufacturer’s instructions (Abcam, ab109721). Briefly, mitochondria were isolated (Mitochondria Isolation Kit, ab110168) from vehicle- and NR-treated

(34) Omaveloxolone, But Not Dimethyl Fumarate, Improves Cardiac Function in Friedreich's Ataxia Mice With Severe CardiomyopathyJournal of the American Heart AssociationJune 17, 2025Lili Salinas, Francisco Figueroa, Claire B. Montgomery, Phung N. Thai, Nipavan Chiamvimonvat, Gino Cortopassi, Elena N. DedkovaMitochondrial enzyme activities were determined with Abcam assays for aconitase (ab109712), Complex I (ab109721), and Complex II (ab109908). Differentiated FA Fibroblasts FA skin fibroblasts were obtained from

(35) Steroid Sulfatase Regulates Metabolic Reprogramming in Advanced Prostate CancerCancers (Basel)June 12, 2025Masuda Sharifi, Cameron M. Armstrong, Shu Ning, Amy R. Leslie, Zachary A. Schaaf, James P. Maine, Wei Lou, Pui-Kai Li, Hongyu Xu, Chengfei Liu, et al.I enzyme activity was performed using the Complex I Enzyme Activity Assay Kit (Colorimetric)(Cat #: ab109721) from Abcam (Cambridge, UK). Two days prior to the assay, adherent cells were plated in 10 cm Petri

(36) Omaveloxolone, But Not Dimethyl Fumarate, Improves Cardiac Function in Friedreich's Ataxia Mice With Severe CardiomyopathyJ Am Heart AssocJune 12, 2025Lili Salinas, Francisco Figueroa, Claire B. Montgomery, Phung N. Thai, Nipavan Chiamvimonvat, Gino Cortopassi, Elena N. DedkovaMitochondrial enzyme activities were determined with Abcam assays for aconitase (ab109712), Complex I (ab109721), and Complex II (ab109908). Differentiated FA Fibroblasts FA skin fibroblasts were obtained from

(37) Mitochondrial PTRH2 controls the deubiquitinase TRABID to regulate mt-ND5 stability and metabolismPNAS NexusMay 31, 2025Carlotta Giorgi, Femke J Aan, Natalija Glibetic, Daniela Ramaccini, Lorenzo Modesti, Veronica A M Vitto, Vanessa Montoya-Uribe, Austin Corpuz, Sonia Missiroli, Inês Simões, et al.activity was assessed using a colorimetric Complex I Enzyme Activity Microplate Assay Kit (Abcam, #AB109721) per manufacturer's instructions. Cell lysates (100 µg) were added to complex I capture plates and enzymatic

(38) OXPHOS inhibition overcomes chemoresistance in triple negative breast cancerRedox BiolApril 15, 2025Cemile Uslu, Eda Kapan, Alex Lyakhovichactivity of mitochondrial OXPHOS Complex I (NADH dehydrogenase) and Complex III were measured with kits (ab109721 and ab109905, respectively, both from Abcam, UK) according to the manufacturer's manual. The specific

(39) Cross-talks between Metabolic and Translational Controls during Beige Adipocyte DifferentiationNat CommunApril 9, 2025Daehwa Youn, Boseon Kim, Dahee Jeong, Ju Yeon Lee, Seha Kim, Dulguun Sumberzul, Rehna Paula Ginting, Min-Woo Lee, Ju Hwan Song, Ye Seul Park, et al.activities were measured with Complex I Enzyme Activity Microplate Assay Kit (Colorimetric) (Abcam, ab109721) and Succinate Dehydrogenase Activity Assay Kit (Colorimetric) (Abcam, ab228560), respectively. Assays

(40) PPDPF preserves integrity of proximal tubule by modulating NMNAT activity in chronic kidney diseasesSci AdvMarch 21, 2025Xiaoliang Fang, Yi Zhong, Rui Zheng, Qihui Wu, Yu Liu, Dexin Zhang, Yuwei Wang, Wubing Ding, Kaiyuan Wang, Fengbo Zhong, et al.subjected to NMNAT1 activity assay (Abcam, #ab221820) and complex I enzyme activity assay (Abcam, #ab109721), following the manufacturer’s instructions. Protein purification PPDPF WT, C30S, C35S, and NMNAT1/2/3

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Complex I Enzyme Activity Microplate Assay Kit (Colorimetric) from Abcam

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Complex I Enzyme Activity Microplate Assay Kit (Colorimetric) from Abcam

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