TUNEL Assay Kit - FITC ab66108 from Abcam

Product Specs
ItemTUNEL Assay Kit - FITC
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab66108
Quantity50 Test
MethodFlow Cyt
Sample TypeAdherent cells, Suspension cells
Assay Time2h 30m

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Abcam

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Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(41) TIA1 Loss Exacerbates Fatty Liver Disease but Exerts a Dual Role in HepatocarcinogenesisCancers (Basel)March 27, 2022Dobrochna Dolicka, Szabolcs Zahoran, Marta Correia de Sousa, Monika Gjorgjieva, Christine Sempoux, Margot Fournier, Christine Maeder, Martine A. Collart, Michelangelo Foti, Cyril Sobolewskithe day of the analysis, the cells were washed and stained as described in the TUNEL FITC Assay Kit (ab66108, Abcam, Cambridge, UK). The cells were then analyzed on CytoFLEX (Beckman Coulter, Brea, CA, USA) and

(42) Profiling Temporal Changes of the Pineal Transcriptomes at Single Cell Level Upon Neonatal HIBDFrontiers in Cell and Developmental BiologyMarch 8, 2022Xin Ding et al.detected no fluorescent signals. TUNEL assay was performed according to the manual (TUNEL-FITC, Abcam, ab66108). DNase I treated or PBS treated sildes were used as a positive and negative control of TUNEL staining,

(43) The potential role of methyltransferase-like 5 in deficient mismatch repair of uterine corpus endometrial carcinomaBioengineeredMarch 1, 2022Liu X, Ma H, Ma L, Li K, Kang Y.end labeling (TUNEL) staining Tissue fractions from each group were analyzed using a TUNEL assay (ab66108, Abcam, Cambridge, UK), as described previously [21]. 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di- phenytetrazoliumromide

(44) Advanced glycation end products promote osteoporosis by inducing ferroptosis in osteoblastsMolecular Medicine ReportsFebruary 24, 2022Weiwei Ge, Jian Jie, Jie Yao, Wei Li, Yahui Cheng, Wenjuan Ludays. TUNEL staining Cell apoptosis was evaluated using a TUNEL assay (TUNEL Assay kit-FITC; cat. no. ab66108; Abcam), according to the manufacturer's instructions. Briefly, following culture in different media

(45) Transmembrane p24 trafficking protein 2 regulates inflammation through the TLR4/NF-κB signaling pathway in lung adenocarcinomaWorld journal of surgical oncologyFebruary 8, 2022Feng L, Cheng P, Feng Z, Zhang X.end labeling (TUNEL) staining Tissue fractions from each group were analyzed using a TUNEL assay (ab66108, Abcam, Cambridge, UK), as described previously [24]. Images were captured using a fluorescence microscope

(46) Adiponectin triggers breast cancer cell death via fatty acid metabolic reprogrammingJournal of experimental & clinical cancer research : CRJanuary 5, 2022Pham DV, Park PH.TUNEL assay TUNNEL assay was carried out to detect late apoptotic cell population using the TUNEL Assay Kit - FITC (#ab66108; Abcam) according to the manufacturer’s guidelines. Briefly, 106 cells were fixed with 1% paraformaldehyde

(47) Icariin-loaded hydrogel with concurrent chondrogenesis and anti-inflammatory properties for promoting cartilage regeneration in a large animal modelFrontiers in cell and developmental biologyJanuary 1, 2022Xu S, Zhao S, Jian Y, Shao X, Han D, Zhang F, Liang C, Liu W, Fan J, Yang Z, et al.against collagen type II, IL-1β, IL-6, TNF-α, and TUNEL (No. ab188570, ab254360, ab303458, ab183218, and ab66108, Abcam) at 4°C. After rinsing, the samples were treated with goat anti-rabbit secondary antibody (1:1000,

(48) Antitumor Potential of Sericite Treatment Mediated by Cell Cycle Arrest in Triple-Negative MDA-MB231 Breast Cancer CellsEvidence-based complementary and alternative medicine : eCAMJanuary 1, 2022Kim S, Nagar H, Lee I, Choi SJ, Piao S, Jeon BH, Kang SK, Song HJ, Kim CS.overnight at 4°C. TUNEL assay was performed according to the manufacturer's instructions (FITC Abcam, cat. ab66108). Stained cells were analyzed by flow cytometry for FITC using a NovoCyte flow cytometer as per the

(49) High doses of dexamethasone induce endoplasmic reticulum stress-mediated apoptosis by promoting calcium ion influx-dependent CHOP expression in osteoblastsMolecular biology reportsDecember 1, 2021Guo Y, Hao D, Hu H.infrared laser imaging system. TUNEL staining Cell apoptosis was detected using the TUNEL Assay Kit (ab66108, Abcam). Cells in suspension were added to the inner cover glass of a cell culture plate and cultured

(50) Callyspongiolide kills cells by inducing mitochondrial dysfunction via cellular iron depletionCommunications biologySeptember 23, 2021Ha J, Park SB.CO2 at 37 °C. DNA fragmentation was observed with in situ direct DNA fragmentation (TUNEL) assay kit (ab66108; Abcam) according to manufacturer instructions. Briefly, after the media was aspirated, the cells were

(51) Effects of low‑dose bufalin combined with hydroxycamptothecin on human castration‑resistant prostate cancer xenografts in nude miceExperimental and Therapeutic MedicineJuly 15, 2021Renze Gu, Qingchuan ZhangMicrosystems Inc.; magnification, x4, x10 and x40). TUNEL detection The TUNEL Assay kit-FITC (cat. no. ab66108; Abcam) was used to detect apoptosis in paraffin-embedded sections, according to the manufacturer's

(52) Lidocaine inhibits the proliferation and metastasis of epithelial ovarian cancer through the Wnt/β-catenin pathwayTranslational Cancer ResearchJuly 1, 2021Mei Sun, Saisai Huang, Yongtao Gaotransferase-mediated digoxigenin-dUTP nick-end labeling (TUNEL) assays TUNEL assay kit was purchased from Abcam (ab66108, Cambridge, USA). Treated cells and fixed cells in 4% PFA, next 0.1% Triton X-100 was used to permeabilize

(53) Cisplatin and Pemetrexed Have Distinctive Growth-inhibitory Effects in Monotherapy and Combination Therapy on KRAS-dependent A549 Lung Cancer CellsCancer Genomics - ProteomicsMay 11, 2021MD MOHIUDDIN, KAZUO KASAHARAor pemetrexed as single-drug therapy or in combination. An in situ Direct DNA Fragmentation (TUNEL) Assay Kit (ab66108; Abcam) was used to measure DNA fragmentation in apoptotic cells. In brief, cells were fixed with 1%

(54) Cisplatin Activates the Growth Inhibitory Signaling Pathways by Enhancing the Production of Reactive Oxygen Species in Non-small Cell Lung Cancer Carrying an EGFR Exon 19 DeletionCancer Genomics - ProteomicsMarch 12, 2021MD MOHIUDDIN, KAZUO KASAHARADNA fragmentation in apoptotic cells, we used the in situ Direct DNA Fragmentation (TUNEL) Assay Kit (ab66108, Abcam), following the manufacturer’s instructions. In brief, cells were fixed with 1% paraformaldehyde

(55) Mechanical competition triggered by innate immune signaling drives the collective extrusion of bacterially infected epithelial cellsDevelopmental cellFebruary 22, 2021Bastounis EE, Serrano-Alcalde F, Radhakrishnan P, Engström P, Gómez-Benito MJ, Oswald MS, Yeh YT, Smith JG, Welch MD, García-Aznar JM, et al.cells retain the stain (Figure S6E). In addition, we used the TUNEL assay for DNA fragmentation (Abcam, ab66108) as a means to detect number of apoptotic cells. For staining cells for DNA fragmentation, we followed

(56) Gut-licensed IFNγ⁺ NK cells drive LAMP1⁺TRAIL⁺ anti-inflammatory astrocytesNatureFebruary 1, 2021Sanmarco LM, Wheeler MA, Gutiérrez-Vázquez C, Polonio CM, Linnerbauer M, Pinho-Ribeiro FA, Li Z, Giovannoni F, Batterman KV, Scalisi G, et al.on ice, cells were washed and fixed to detect active-caspase-3 as described above. For TUNEL (Abcam, ab66108) staining, cells were fixed in 1% v/v PFA for 15 min on ice, washed with 1× PBS and incubated with 70%

(57) Small Molecule Cjoc42 Improves Chemo-Sensitivity and Increases Levels of Tumor Suppressor Proteins in Hepatoblastoma Cells and in Mice by Inhibiting Oncogene GankyrinFrontiers in pharmacologyJanuary 1, 2021D'Souza AM, Cast A, Kumbaji M, Rivas M, Gulati R, Johnston M, Smithrud D, Geller J, Timchenko N.chemotherapy plus 1, 5, and 10 µM of Cjoc42 at a final dilution of 0.1% DMSO for 48–72 h. TUNEL Assay (Abcam, ab66108) was conducted on untreated Huh6 cells, cells treated with cisplatin, cells treated with Cjoc42, and

(58) Circ_0030235 knockdown protects H9c2 cells against OGD/R-induced injury via regulation of miR-526bPeerJJanuary 1, 2021Zhang Y, Liu S, Ding L, Wang D, Li Q, Li D.with shRNA or miRNA inhibitor. Internucleosomal DNA fragmentation was detected using TUNEL Assay Kit (ab66108; Abcam, Cambridge, UK) to assess cell apoptotic potential. In brief, the adherent cultured H9c2 cells

(59) Azeliragon ameliorates Alzheimer's disease via the Janus tyrosine kinase and signal transducer and activator of transcription signaling pathwayClinics (Sao Paulo, Brazil)January 1, 2021Yang L, Liu Y, Wang Y, Li J, Liu N.antibodies Tofacitinib (S5001, Selleck, Texas, USA), fludarabine (S1491, Selleck, Texas, USA), TUNEL reaction solution (ab66108, Abcam, Cambridge, UK), TTP488 (HY-50682, MedChem Express, Sollentuna, Sweden), LPS (L8880, Solarbio,

(60) Pharmacological Activation of Autophagy Restores Cellular Homeostasis in Ultraviolet-(B)-Induced Skin PhotodamageFrontiers in oncologyJanuary 1, 2021Umar SA, Shahid NH, Nazir LA, Tanveer MA, Divya G, Archoo S, Raghu SR, Tasduq SA.Bio-Rad). TUNEL Assay TUNEL assay was performed with an In Situ Direct DNA Fragmentation (TUNEL) Assay Kit (ab66108) from (Abcam) according to the manufacturer’s instructions as described previously (19). Briefly, the

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TUNEL Assay Kit - FITC from Abcam

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TUNEL Assay Kit - FITC from Abcam

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