TUNEL Assay Kit - FITC ab66108 from Abcam

Product Specs
ItemTUNEL Assay Kit - FITC
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab66108
Quantity50 Test
MethodFlow Cyt
Sample TypeAdherent cells, Suspension cells
Assay Time2h 30m

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(21) Efficient pulmonary lymphatic drainage is necessary for inflammation resolution in ARDSJCI insightJanuary 9, 2024Zhang PH, Zhang WW, Wang SS, Wu CH, Ding YD, Wu XY, Smith FG, Hao Y, Jin SW.and Ki-67 (rabbit, 1:200; Thermo Fisher Scientific, catalog PA5-19462), or TUNEL-FITC (Abcam, catalog ab66108). The corresponding secondary antibodies were used: goat anti-rabbit DyLight 594 (1:200, Invitrogen,

(22) Long non-coding RNA growth arrest-specific 5 inhibits liver fibrogenesis in biliary atresia by interacting with microRNA-222 and repressing IGF1/AKT signalingTranslational PediatricsDecember 26, 2023Ruoyi Wang, Ya Gaomin. Next, sections were washed three times with PBS and incubated in FITC-marked 50 µL TUNEL (Abcam, ab66108) at 37 °C for 1 h. Finally, TUNEL staining was observed using a fluorescence microscope (Leica DM IL LED

(23) Mitochondrial H2O2 Is a Central Mediator of Diclofenac-Induced Hepatocellular InjuryAntioxidants (Basel)December 21, 2023Sin Ri Kim, Ji Won Park, You-Jin Choi, Seong Keun Sonn, Goo Taeg Oh, Byung-Hoon Lee, Tong-Shin Chang20 min of permeabilization in 0.25 percent Triton-X100 at room temperature, the TUNEL test kit-FITC (ab66108, Abcam, Cambridge, UK) was used to mark apoptotic cells. The fluorescence signal was obtained using

(24) A secreted form of chorismate mutase (Rv1885c) in Mycobacterium bovis BCG contributes to pathogenesis by inhibiting mitochondria-mediated apoptotic cell death of macrophagesJ Biomed SciDecember 18, 2023Mi-Hyun Lee, Hye Lin Kim, Hyejun Seo, Sangkwon Jung, Bum-Joon Kim× 105) were seeded in 2-well chamber slides (Thermo Scientific, 154461). A TUNEL assay kit (Abcam, ab66108) was used to detect DNA fragmentation and apoptotic cells. Infected BMDMs were fixed with 4% PFA for

(25) The abscission checkpoint senses chromatin bridges through Top2α recruitment to DNA knotsThe Journal of cell biologyNovember 6, 2023Petsalaki E, Balafouti S, Kyriazi AA, Zachos G.and processed for immunofluorescence. TUNEL assay and immunofluorescence For TUNEL staining, the ab66108 in situ Direct DNA Fragmentation (TUNEL) Assay Kit (Abcam; Fig. 1; Fig. 7 B; Fig. S2 E, F, and I; and

(26) Dysregulation of a lncRNA within the TNFRSF10A locus activates cell death pathwaysCell Death DiscoveryJuly 13, 2023Tadeusz J. Kaczynski, Nadine J. Husami, Elizabeth D. Au, Michael H. Farkastransferase dUTP nick end labeling TUNEL staining was carried out using a FITC TUNEL assay kit (Abcam, Cat#: ab66108) according to the manufacturer’s instructions with minor alterations. Briefly, ARPE-19 cells were collected

(27) Post‐inflammatory administration of N‐acetylcysteine reduces inflammation and alters receptor levels in a cellular model of Parkinson's diseaseFASEB BioAdvancesJuly 1, 2023Zeynep Bengisu Kaya, Elif Karakoc, Pamela J. McLean, Esen Saka, Pergin Atillaapoptotic cells. The TUNEL evaluation was carried out using a TUNEL experimental kit (TUNEL Assay Kit‐FITC ab66108; Abcam) according to the manufacturer's instructions. Cells were cultured on 6‐cm petri dishes without

(28) Diplacone Isolated from Paulownia tomentosa Mature Fruit Induces Ferroptosis-Mediated Cell Death through Mitochondrial Ca2+ Influx and Mitochondrial Permeability TransitionInternational Journal of Molecular SciencesApril 11, 2023Eun Sol Oh, Hyung Won Ryu, Yu Na Song, Myung-Ji Kang, Yang Hoon Huh, Ji-Yoon Park, Seon Min Oh, Su-Yeon Lee, Yhun Jung Park, Doo-Young Kim, et al.was purchased from Enzo Life Sciences (Farmingdale, NY, USA). The FITC-conjugated TUNEL Assay Kit (ab66108) and lipid peroxidation (MDA) assay kit (ab118972) were acquired from Abcam. 4.2. Plant Material Sample

(29) Mammalian Ste20-like kinase 1 inhibition as a cellular mediator of anoikis in mouse bone marrow mesenchymal stem cellsWorld Journal of Stem CellsMarch 26, 2023Tao Zhang, Qian Zhang, Wan-Cheng YuFragmentation Terminal-deoxynucleoitidyl Transferase Mediated Nick End Labeling (TUNEL) Assay Kit [ab66108, Abcam, The United Kingdom of Great Britain and Northern Ireland (UK)]. After incubation in poly-HEMA-coated

(30) The Effects of Glutathione on Clinically Essential Fertility Parameters in a Bleomycin Etoposide Cisplatin Chemotherapy ModelLife (Basel)March 17, 2023Hale Bayram, Yaprak Donmez Cakil, Mustafa Erinc Sitar, Gamze Demirel, Belgin Selam, Mehmet Cincikdeoxynucleotidyl transferase dUTP nick end labeling) method using the Abcam TUNEL assay kit-FITC (CAS: 3326-32-7; ab66108; Cambridge, UK). First, the sperm were fixed in 1% paraformaldehyde for 15 min at −20 °C. The fixed

(31) Progression of Thoracic Aortic Dissection Is Aggravated by the hsa_circ_0007386/miR-1271-5P/IGF1R/AKT Axis via Induction of Arterial Smooth Muscle Cell ApoptosisBiomedicinesFebruary 15, 2023Xinsheng Xie, Xiang Hong, Shichai Hong, Yulong Huang, Gang Chen, Yihui Chen, Yue Lin, Weifeng Lu, Weiguo Fu, Lixin WangTUNEL staining assay was performed according to the method steps provided by the TUNEL Assay Kits (ab66108, Abcam, USA). Western blot assays were performed as previously described [20]. Detailed methods are

(32) Gut Microbiota-Derived Glutamine Attenuates Liver Ischemia/Reperfusion Injury via Macrophage Metabolic ReprogrammingCellular and molecular gastroenterology and hepatologyJanuary 1, 2023Lu T, Li Q, Lin W, Zhao X, Li F, Ji J, Zhang Y, Xu N.apoptotic cells were detected using the in situ BrdU-Red DNA fragmentation (TUNEL) assay kit (Abcam，cat. #ab66108) and counterstained with DAPI (Tocris, cat. #5748). Immunohistochemistry Paraffin liver sections were

(33) Mechanism of paracrine communications between hepatic progenitor cells and endothelial cellsCellular signallingDecember 1, 2022Lee S, Karns R, Shin S.respectively. The BrdU Cell Proliferation Assay Kit (2750, Millipore, MA, USA) and the TUNEL Assay Kit - FITC (ab66108, Abcam, Cambridge, UK) were used per the manufacturer’s manual. 2.6. Tubulogenesis assay The In Vitro Angiogenesis Assay Kit

(34) Empagliflozin attenuates cardiac microvascular ischemia/reperfusion injury through improving mitochondrial homeostasisCardiovascular DiabetologyDecember 1, 2022Rongjun Zou, Wanting Shi, Junxiong Qiu, Na Zhou, Na Du, Hao Zhou, Xinxin Chen, Li Maantibodies. Images were acquired using a confocal microscope (LCS-SP8-STED, Leica). The TUNEL assay (Abcam, #ab66108) was conducted based on the manufacturer’s instructions. MtROS levels in HCAECs were measured using

(35) Mitochondrial derived peptide MOTS‐c prevents the development of heart failure under pressure overload conditions in miceJournal of Cellular and Molecular MedicineNovember 1, 2022Peng Zhong, Jianye Peng, Yewen Hu, Jun Zhang, Caijie Shenvascular area. Apoptosis was assessed using the TdT‐mediated dUTP nick end labeling (TUNEL) assay kit (ab66108 from Abcam), and slides were viewed using a fluorescence microscope (Olympus). Percentage of TUNEL‐positive

(36) The ex vivo human translaminar autonomous system to study spaceflight associated neuro-ocular syndrome pathogenesisNPJ microgravityOctober 28, 2022Peng M, Curry SM, Liu Y, Lohawala H, Sharma G, Sharma TP.the sections were stained according to manufacturer’s protocol for the TUNEL assay (catalog number ab66108, Abcam). The detection kit utilizes terminal deoxynucleotidyl transferase (TdT) to catalyze incorporation

(37) Checkpoint Kinase 2 Inhibition Can Reverse Tamoxifen Resistance in ER-Positive Breast CancerInternational journal of molecular sciencesOctober 14, 2022Tsoi H, Tsang WC, Man EPS, Leung MH, You CP, Chan SY, Chan WL, Khoo US.USA). ROS level was determined by CM-H2DCFDA (C6827; ThermoFisher, Waltham, MA, USA). TUNEL assay kit (ab66108; Abcam, Cambridge, UK) was employed to determine DNA damage. Absorbance and fluorescent signals were

(38) Dual specificity phosphatase 1 attenuates inflammation-induced cardiomyopathy by improving mitophagy and mitochondrial metabolismMolecular metabolismOctober 1, 2022Tan Y, Zhang Y, He J, Wu F, Wu D, Shi N, Liu W, Li Z, Liu W, Zhou H, et al.(#ab232589, Abcam) was used at a 1:500 dilution. TUNEL staining was performed with a TUNEL Assay Kit (FITC; #ab66108, Abcam) as we previously described [14]. 4.12 Mitochondrial membrane potential, mitochondrial ROS and

(39) Achaete-scute complex-like 2 regulated inflammatory mechanism through Toll-like receptor 4 activating in stomach adenocarcinomaWorld journal of surgical oncologyAugust 25, 2022Zheng E, Cai Z, Li W, Ni C, Fang Q.using an Olympus BX41 fluorescence microscope. Image quantification is analyzed using a TUNEL assay (ab66108, Abcam, Cambridge, UK), as described previously. MTT MTT assays are performed to assess cell proliferation

(40) Hyperglycemia aggravates ischemic brain damage via ERK1/2 activated cell autophagy and mitochondrial fissionFront Endocrinol (Lausanne)August 5, 2022Ping Liu, Xiao Yang, Jianguo Niu, Changchun Heiexperiment. Professor Andy Li assisted this part. TUNEL assay According to the manufacture’s instructions (ab66108,Abcam), the brain cells were rinsed with 1 x PBS, incubated in ice with membrane penetrating solution,

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TUNEL Assay Kit - FITC from Abcam

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TUNEL Assay Kit - FITC from Abcam

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