MHC class II (15G4) Antibody sc-53946 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemMHC class II (15G4) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $316.00Supplier Page View Company Product Page
Catalog Numbersc-53946
Antibody TypePrimary Antibodies
Quantity200 µg/ml
Clone15G4
ApplicationsIP, IF, FCM
Reactivitymouse
Hostmouse
Conjugate/TagUnconjugated
ClonalityMonoclonal
FormatPurified
IsotypeIgG1
NCBI Full Gene Namehistocompatibility 2, class II antigen E alpha
NCBI Gene AliasesE-alpha-f, H-2Ea, H2-Ea-ps, I-Ealpha, Ia-3, Ia3, MHC-H2-Ea
TargetMHC class II

Questions and Answers

Can this MHC class II antibody be used for flow cytometry applications?

Yes, the MHC class II (15G4) antibody has been shown to work effectively in flow cytometry applications, allowing for the analysis of histocompatibility 2, class II antigen E alpha expression on mouse cells.

Is this antibody suitable for immunofluorescence studies?

The MHC class II (15G4) antibody is suitable for immunofluorescence applications, as indicated by its use in published studies to visualize the expression of MHC class II molecules in mouse tissues.

What type of samples can I use this antibody on?

This antibody is specifically reactive with mouse samples, making it appropriate for experiments involving mouse tissues or cell lines expressing the H2-Ea gene.

How does the isotype of this antibody affect its use in immunoprecipitation?

The MHC class II (15G4) antibody is an IgG1 isotype, which is commonly used in immunoprecipitation applications. Its monoclonal nature allows for specific binding to the target antigen, facilitating the isolation of MHC class II complexes from mouse samples.

Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) LAMP2 regulates autophagy in the thymic epithelium and thymic stroma-dependent CD4 T cell developmentAutophagyFebruary 1, 2023Rodrigues PM, Sousa LG, Perrod C, Maceiras AR, Ferreirinha P, Pombinho R, Romera-Cárdenas G, Gomez-Lazaro M, Senkara M, Pistolic J, et al.

(2) Regulation of the BCR signalosome by the class II peptide editor, H2-M, affects the development and repertoire of innate-like B cellsCell reportsJanuary 25, 2022Ghosh D, Pham TD, Nanaware PP, Sengupta D, Adler LN, Li CG, He X, O'Mara ME, Kantor AB, Nguyen KD, et al.

(3) Pleiotropic consequences of metabolic stress for the major histocompatibility complex class II molecule antigen processing and presentation machineryImmunityApril 13, 2021Clement CC, Nanaware PP, Yamazaki T, Negroni MP, Ramesh K, Morozova K, Thangaswamy S, Graves A, Kim HJ, Li TW, et al.,ThermoFisher Scientific,Catalog # 17-5321-82 CD3-AF700,BD Pharmingen,Catalog # 56-0033-82 I-Ab-CLIP,Santa Cruz,Catalog # sc-53946 H2-M,Denzin Lab,Clone YoDMA.1 H2-Ob,Denzin Lab,Clone Ob.1 CD16/32 blocker,Biolegend,Catalog # 101320 CD172a-Alexa

(4) Strength of tonic T cell receptor signaling instructs T follicular helper cell-fate decisionsNature immunologyNovember 1, 2020Bartleson JM, Viehmann Milam AA, Donermeyer DL, Horvath S, Xia Y, Egawa T, Allen PM.HK1.4; Biolegend; cat. no. 128013), anti-CLIP (FITC; clone 15G4; Santa Cruz Biotechnology; cat. no. sc-53946 FITC; 1:50 dilution), anti-CD11c (PE-Cy7; clone N418; Biolegend; cat. no. 117318), anti-CD16/CD32 Fc

Write a Review

(1)

6/30/2015High background level Sherin RWe used this antibody to evaluate whether cells expressed an increased level of MHC II molecules containing the CLIP peptide on their surface.

MHC class II (15G4) Antibody

MHC class II (15G4) Antibody from Santa Cruz Biotechnology, Inc.

Description

Questions and Answers

Santa Cruz Biotechnology, Inc.

Citations (4)

Reviews

MHC class II (15G4) Antibody from Santa Cruz Biotechnology, Inc.

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