Santa Cruz Biotechnology
CLIP antibody
sc-53946 PE
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We used this antibody to evaluate whether cells expressed an increased level of MHC II molecules containing the CLIP peptide on their surface.
Flow Cytometry
Primary murine CD45+ and CD45- cells
1:5 dilution for 30 minutes at 4 degrees C
2.4G2 Fc block
NA
Canto II
The antibody stained well on CD45+ DC and B cells. We used the isotype control from Santa Cruz or MHC II knockout mice as negative controls as there was a high background with an FMO. Additionally, there was a high background staining of both the antibody and the isotype control on non-hematopoietically derived cells including endothelial cells, necessitating the use of MHC II KO mice as a negative control.
None
Good staining on hematopoeitic cells
Can have a high level of background
You need a good negative control such as MHC II KO mice or a non I-Ab expressing mouse strain.