Galectin-3 Antibody AF1154 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemGalectin-3 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF1154
NCBI Gene AliasesGALBP, MAC2, GAL3, GALIG, CBP35, L31, LGALS2
ApplicationsELISA Development (Detection),Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Simple Western,Intracellular Staining by Flow Cytometry,Immunocytochemistry,CyTOF-ready,ELISA Capture
TargetGalectin-3
IsotypeIgG
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
HostGoat
ReactivityHuman,Monkey
NCBI Full Gene Namegalectin 3
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human Galectin-3, Ala2-Ile250, Accession # P17931.5
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human Galectin-3 in direct ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study the role of galectin-3 in pregnancy-related complications?

Yes, the Galectin-3 Antibody has been utilized in studies investigating its role in fetal growth restriction due to placental insufficiency. Research has shown that galectin-3 deficiency during pregnancy can lead to placental dysfunction and increased risk of fetal growth restriction, highlighting its importance in placental function and fetal development ().

Is this antibody effective for detecting galectin-3 in immunofluorescence applications?

The Galectin-3 Antibody has been successfully employed in immunofluorescence assays, as demonstrated in studies where it was used to visualize galectin-3 expression in various cell types, including Caco-2 cells and in tissue sections from patients with atopic dermatitis (, ).

How does this antibody perform in Western blotting applications?

The Galectin-3 Antibody has been shown to effectively detect human galectin-3 in Western blotting applications, as evidenced by its use in various studies to analyze galectin-3 levels in different biological contexts, including tumor microenvironments and cell culture supernatants (, ).

What species does this antibody react with, and is it suitable for human studies?

This antibody is specifically reactive with human galectin-3, making it suitable for studies involving human samples, including those examining immune responses and cellular interactions in human tissues ().

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Smooth Muscle Cell‐Derived Fibronectin Promotes an Atheroprotective Smooth Muscle Cell Phenotype Associated With Altered NO‐cGMP SignalingJournal of the American Heart AssociationJune 3, 2025Susanne Feil, Maria T. K. Zaldivia, Jacek Kiesel, Malte Roessing, Andreas Peter, Daniel Morales‐Cano, Jacob F. Bentzon, Moritz Lehners, Robert Feilstained with hematoxylin/eosin or immunostained with antibodies recognizing human LGALS3 (R&D Systems, AF1154, from goat) or GUCY1B1 (Abcam, ab24824, from rabbit). Staining was documented using an Axioskop 20 (Zeiss)

(2) Smooth Muscle Cell‐Derived Fibronectin Promotes an Atheroprotective Smooth Muscle Cell Phenotype Associated With Altered NO‐cGMP SignalingJ Am Heart AssocMay 29, 2025Susanne Feil, Maria T. K. Zaldivia, Jacek Kiesel, Malte Roessing, Andreas Peter, Daniel Morales‐Cano, Jacob F. Bentzon, Moritz Lehners, Robert Feilstained with hematoxylin/eosin or immunostained with antibodies recognizing human LGALS3 (R&D Systems, AF1154, from goat) or GUCY1B1 (Abcam, ab24824, from rabbit). Staining was documented using an Axioskop 20 (Zeiss)

(3) TECPR1 is activated by damage-induced sphingomyelin exposure to mediate noncanonical autophagyThe EMBO journalSeptember 4, 2023Kaur N, de la Ballina LR, Haukaas HS, Torgersen ML, Radulovic M, Munson MJ, Sabirsh A, Stenmark H, Simonsen A, Carlsson SR, et al.(Mu et al, 1995), FLAG epitope M2 mouse mAb (Sigma F1804, 1:500), Galectin‐3 goat pAb (R&D Systems, AF1154, 1:300), LAMP1 (clone H4A3) mouse mAb (H4A3 was deposited to the DSHB by August, J.T./Hildreth, J.E.K.

(4) Biomimetic nanoparticles drive the mechanism understanding of shear-wave elasticity stiffness in triple negative breast cancers to predict clinical treatmentBioactive materialsApril 1, 2023Zheng D, Zhou J, Qian L, Liu X, Chang C, Tang S, Zhang H, Zhou S.

(5) A multifaceted role of progranulin in regulating amyloid-beta dynamics and responsesLife science allianceJuly 1, 2021Du H, Wong MY, Zhang T, Santos MN, Hsu C, Zhang J, Yu H, Luo W, Hu F.anti-β-Amyloid (25524-1-AP; Proteintech Group), mouse anti-galectin-3 (126702; BioLegend), goat anti–galectin-3 (AF1154; R&D Systems), goat anti-GPNMB (AF2330; R&D Systems), mouse anti-GAPDH (60004-1-Ig; Proteintech Group),

(6) Network analysis of the progranulin-deficient mouse brain proteome reveals pathogenic mechanisms shared in human frontotemporal dementia caused by GRN mutationsActa neuropathologica communicationsOctober 7, 2020Huang M, Modeste E, Dammer E, Merino P, Taylor G, Duong DM, Deng Q, Holler CJ, Gearing M, Dickson D, et al.R&D Systems), anti-mouse Galectin-3 (Goat, AF1197, 1:1000, R&D Systems), anti-human Galectin-3 (Goat, AF1154, 1:1000, R&D Systems), or anti-human GPNMB (Goat, AF2550, 1:1000, R&D Systems) antibody. All primary antibodies

(7) Alpha-Gal on the Protein Surface Hampers Transcytosis through the Caco-2 MonolayerInternational journal of molecular sciencesAugust 11, 2020Krstić Ristivojević M, Grundström J, Apostolović D, Radomirović M, Jovanović V, Radoi V, Kiewiet MBG, Vukojević V, Ćirković Veličković T, van Hage M.was performed using goat polyclonal anti-human galectin-3 IgG antibody (R&D systems, Abingdon, UK, AF1154) diluted 1:10,000 in 0.1% BSA for 1 h at RT followed by rabbit anti-goat IgG conjugated to AP (Sigma-Aldrich,

(8) Galectin-3 deficiency in pregnancy increases the risk of fetal growth restriction (FGR) via placental insufficiencyCell death & diseaseJuly 23, 2020Freitag N, Tirado-Gonzalez I, Barrientos G, Powell KL, Boehm-Sturm P, Koch SP, Hecher K, Staff AC, Arck PC, Diemert A, et al.plates (Dynatech Laboratories, USA) were covered with polyclonal antihuman gal-3 antibody (1 μg/ml; AF1154, R&D Systems, USA) and washed with washing buffer (0.5% Tween-20 in PBS). Plates were blocked with 3%

(9) Galectin-Levels Are Elevated in Infants Born Preterm Due to Amniotic Infection and Rapidly Decline in the Neonatal PeriodFrontiers in immunologyJanuary 1, 2020Faust K, Freitag N, Barrientos G, Hartel C, Blois SM.Infants TABLE 1 | Clinical characteristics of the included preterm infants. antibodies (500-P210 Petrotech, AF1154 and AF 2045; R&D Systems, USA respectively) and washed with washing buffer (0.5% Tween-20 in PBS). Plates

(10) Human trophoblast requires galectin-3 for cell migration and invasionScientific reportsFebruary 14, 2019Bojić-Trbojević Ž, Jovanović Krivokuća M, Vilotić A, Kolundžić N, Stefanoska I, Zetterberg F, Nilsson UJ, Leffler H, Vićovac L.human galectin-3 (rhgalectin-3), recombinant human galectin-8 (rhgalectin-8), goat anti-galectin-3 (AF1154) and goat anti-galectin-8 (AF1305) antibodies were from R&D Systems (Abingdon UK). The mutant form of

(11) ESCRT-mediated lysosome repair precedes lysophagy and promotes cell survivalThe EMBO journalNovember 2, 2018Radulovic M, Schink KO, Wenzel EM, Nähse V, Bongiovanni A, Lafont F, Stenmark H.antibodies were described previously (Christ et al, 2016). Goat anti-Galectin-3 antibody (cat. no. AF1154) and human Galectin-3 Alexa Fluor 488-conjugated antibody (cat. no. IC1154G) were purchased from R&D Systems

(12) Intranuclear accumulation of galectin-3 is an independent prognostic factor for patients with distal cholangiocarcinomaOncology lettersJuly 1, 2017Shimura T, Kofunato Y, Okada R, Yashima R, Koyama Y, Araki K, Kuwano H, Takenoshita S.sections were incubated with polyclonal goat anti‑galectin‑3 antibody (dilution, 1:2,000; catalog no., AF1154; R&D Systems, Inc., Minneapolis, MN, USA) overnight at 4˚C. An additional wash in PBS was followed by

(13) Group 2 Innate Lymphoid Cells Express Functional NKp30 Receptor Inducing Type 2 Cytokine ProductionJournal of immunology (Baltimore, Md. : 1950)January 1, 2016Salimi M, Xue L, Jolin H, Hardman C, Cousins DJ, McKenzie AN, Ogg GS.isotype control (Abcam, ab37416), mouse anti-rabbit HRP (344002, MaxDiscovery), and anti–Galectin-3 Ab (AF1154, Bio-Techne) were used to stain formalin-fixed, paraffinembedded skin tissue sections from healthy donors

(14) Expression of immune checkpoint molecules in endometrial carcinomaExperimental and therapeutic medicineNovember 1, 2015Liu J, Liu Y, Wang W, Wang C, Che Y.commercial ELISA kit (R&D Systems, Inc.) with only minor changes, and mouse anti‑human galectin‑3 (2 µg/ml; AF1154; R&D Systems, Inc.) was used to detect the expression levels. The standard concentration of galectin‑3

(15) Deciphering the complex three-way interaction between the non-integrin laminin receptor, galectin-3 and Neisseria meningitidisOpen biologyOctober 1, 2014Alqahtani F, Mahdavi J, Wheldon LM, Vassey M, Pirinccioglu N, Royer PJ, Qarani SM, Morroll S, Stoof J, Holliday ND, et al.available Gal-3-specific mouse monoclonal (MAb; Mac-2 clone M3/38; Biolegends) and goat polyclonal (PAb; AF1154; R&D Systems) antibodies were also employed. Staining of non-permeabilized hBMEC cells clearly demonstrated

(16) Tonsil-derived mesenchymal stem cells alleviate concanavalin A-induced acute liver injuryExperimental cell researchAugust 1, 2014Ryu KH, Kim SY, Kim YR, Woo SY, Sung SH, Kim HS, Jung SC, Jo I, Park JW.

(17) "Modulation of endothelial cell migration and angiogenesis: a novel function for the ""tandem-repeat"" lectin galectin-8"FASEB journal : official publication of the Federation of American Societies for Experimental BiologyJanuary 1, 2011Delgado VM, Nugnes LG, Colombo LL, Troncoso MF, Fernández MM, Malchiodi EL, Frahm I, Croci DO, Compagno D, Rabinovich GA, et al.

(18) Tumor-Associated Galectin-3 Modulates the Function of Tumor-Reactive T CellsCancer ResearchSeptember 1, 2008Weiyi Peng, Helen Y. Wang, Yoshihiro Miyahara, Guangyong Peng, Rong-Fu Wangusing standard methods. The paired antibodies for galectin-3 ELISA assay are anti-human galectin-3 (AF1154) and Biotin conjugated anti-human galectin-3 (BAF1154) from R&D system. Each reported value is the mean

(19) TUMOR ASSOCIATED GALECTIN-3 MODULATES THE FUNCTION OF TUMOR-REACTIVE T CELLSnihpaSeptember 1, 2008Weiyi Peng, Helen Y Wang, Yoshihiro Miyahara, Guangyong Peng, Rong-Fu Wangusing standard methods. The paired antibodies for galectin-3 ELISA assay are anti-human galectin-3 (AF1154) and Biotin conjugated anti-human galectin-3 (BAF1154) from R&D system. Each reported value is the mean

(20) Sialylation of beta1 integrins blocks cell adhesion to galectin-3 and protects cells against galectin-3-induced apoptosisThe Journal of biological chemistryAugust 8, 2008Zhuo Y, Chammas R, Bellis SL.

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(1)

2/11/2015Galectin-3 for immunohistochemistry, human colon Rocky SateThis product was used for detection of Galectin-3 in human colon epithelial cells. Galectin-3 is a lectin that is highly expressed in cancers and is also found in normal epithelial cells. To detect ... Read Review
(1)

2/10/2015Galectin-3 Antibody for Flow Cytometry Rocky SateThis product was used for Galectin-3 detection in colon cancer cells using flow cytometry. Galectin-3 is a lectin that is highly expressed in cancers and is also found in normal epithelial cells. ... Read Review