TNFα (52B83) Antibody sc-52746 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemTNFα (52B83) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $316.00Supplier Page View Company Product Page
Catalog Numbersc-52746
Quantity100 µg/ml
ApplicationsWB, IP, IF, IHC(P), FCM, ELISA
Reactivitymouse, rat, human
FormatPurified
Conjugate/TagUnconjugated
Antibody TypePrimary Antibodies
Clone52B83
Hostmouse
TargetTNFalpha
IsotypeIgG1
NCBI Gene AliasesTNFalpha, TNFA, DIF, Tnfsf1a, TNF-a, Tnfa, TNFSF2, TNF-alpha, TNLG1F, Tnlg1f
ClonalityMonoclonal
NCBI Full Gene Nametumor necrosis factor

Questions and Answers

What species does this TNFα antibody work with in experiments?

The TNFα (52B83) antibody has been shown to be reactive with mouse, human (Homo sapiens), and rat species.

In what types of applications has this antibody been utilized according to the literature?

This antibody has been utilized in various applications including Western blotting, ELISA, flow cytometry, immunofluorescence, immunohistochemistry, and immunoprecipitation.

Are there any specific studies that have used this antibody in their research?

Yes, the TNFα antibody (sc-52746) has been cited in numerous studies, including those investigating the role of TNFα in inflammation and apoptosis, such as in models of metabolic syndrome and colitis.

How has this antibody contributed to understanding the mechanisms of inflammation in experimental models?

The TNFα antibody has been used to assess the levels of TNFα in various experimental models, such as in studies examining the inflammatory response in colitis and the effects of mesenchymal stem cells on inflammation, thereby providing insights into the role of TNFα in these processes.

Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) In vitro evidence that plasma of women with eclampsia disrupts the blood-brain barrierFrontiers in PhysiologyApril 27, 2026Jesenia Acurio, Felipe Troncoso, Esthefanny Escudero-Guevara, Hermes Sandoval, Belen Ibañez, Manu Vatish, Pablo Torres-Vergara, Lina Bergman, Carlos Escuderoused to analyze the presence of potential BBB modulators, including TNF-α (Santa Cruz Biotechnology, sc-52746) and eNOS (BD Transduction Laboratories, Becton, NJ, USA; 610299), by Western blot. For these Western blot

(2) Protective Effects of a Propolis– Petasites japonicus Mixture on Scopolamine-Induced Memory Impairment in MiceJournal of Microbiology and BiotechnologyApril 27, 2026Mi Yeung Kim, Jae Young Shin, Jun Hyeong Lim, Ji Hyeon Park, Yong Kap Hur, Soon-Il Yun, Byoung Ok Cho, Seon Il Jang(sc-166748), p-JNK (sc-6254), JNK (sc-7345), p-p38 (sc-7973), p38 (sc-81621), Tau (sc-32274), and TNF-α (sc-52746) were obtained from Santa Cruz Biotechnology (USA). The COX-2 antibody (160126) was purchased from Cayman Chemical

(3) Hepatoprotective Effects of Black Ginseng Extract and Ginsenoside Rh1 Against Alcohol-Induced Liver Injury: Mechanistic Insights from Network Pharmacology, In Vitro, and In Vivo AnalysisAntioxidants (Basel)April 8, 2026Hyeon Seon Na, Jeon Hwang-Bo, Woo-Cheol Shin, Jin-Kyu Jang, Bo-Ram Choi, Dae Young LeeDallas, TX, USA Cat no. sc-57315, Lot no. I0816), anti-TNF-α (Santa Cruz, Dallas, TX, USA, Cat no. sc-52746, Lot no. C0119), and anti-HO-1 (Abcam, Waltham, MA, USA, Cat no. ab13243). Peroxidase-conjugated anti-rabbit

(4) Ambrisentan attenuates cisplatin-related mitochondrial dysfunction in the heart via regulation of p53 and NF-κB signalingScientific ReportsMarch 24, 2026Hnin Ei Ei Khine, Supachoke Mangmool, Warisara Parichatikanondincubated with specific primary antibodies including TNFα (Santa Cruz Biotechnology, Santa Cruz, CA, USA; sc-52746, RRID:AB_630341), OPA1 (sc-393296, RRID:AB_3101815), DNM1 (sc-12724, RRID:AB_2230650), NRF1 (sc-515360,

(5) Spirulina Preconditioning Attenuates Ischemia-Reperfusion Injury in a Steatotic Rat Liver ModelAntioxidants (Basel)March 19, 2026Eya Baily, Kamel Mhalhel, Soumaya Ben Ahmed, Mohamed Amine Zaouali, Giuseppe Montalbano, Ines Naouar, Antonino Germanà, Hassen Ben AbdennebiNumber,Source,Dilution TNF alpha antibody (52B83),"Santa Cruz Biotechnology, Santa Cruz, CA, USA",sc-52746,Mouse monoclonal,1:100 IL-1β antibody (E7-2-hIL1β),"Santa Cruz Biotechnology, CA, USA",sc-32294,mouse

(6) Microneedle patches deliver targeted GLP-1RAs-loaded nanoparticles for the treatment of rheumatoid arthritisJ NanobiotechnologyMarch 16, 2026Hongyu Zhang, Yao Liu, Shiyu Zhang, Zuli Wang, Xia Zhang, Yang Zhang, Ji Zhang, Shenju Liang, Changqing Li, Min Su, et al.was obtained from ABclonal (Wuhan, China). Anti-cGAS (cat. no. sc-515777) and anti-TNF-α (cat. no. sc-52746) antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Goat anti-Rabbit IgG (Alexa

(7) Nephroprotective effects of visnagin through modulation of macrophage polarization, oxidative stress, inflammation and apoptosis in renal I/R injuryOpen Med (Wars)March 9, 2026Suleyman Sagir, Ugur Seker, Merve Pekince-Ozoner, Meral Yuksel, Gul Sahika Gokdemir, Seval Kaya, Mehmet DemirCatalog no: sc-56053), pro-inflammatory TNF-α (Santa Cruz Biotechnology, Dallas, TX, USA; Catalog no: sc-52746), and M1/M2 macrophage polarization markers CD86 (Santa Cruz Biotechnology, Dallas, TX, USA; Catalog

(8) Connexin 43 and Pannexin 1 in Renal Cell Populations in Diabetic Kidney DiseaseInternational Journal of Molecular SciencesFebruary 25, 2026Marinela Jelinčić Korčulanin, Anita Racetin, Nikola Pavlović, Ivo Jeličić, Merica Glavina Durdov, Monika Andrzejewska, Leo Jerčić, Ivana Bočina, Nives Kević, Ivana Restović, et al.(C4),sc-47778,Mouse,1:1000,"Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA" Primary,TNF alpha Antibody (52B83),sc-52746,Mouse,1:1000,"Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA" Primary,TGF beta 1 Antibody (3C11),sc-130348,Mouse,1:1000,"Santa Cruz Biotechnology Inc

(9) High-intensity interval training remodels perineuronal nets in the medial prefrontal cortex to drive microglial polarization and alleviate osteoarthritis painScientific ReportsFebruary 20, 2026Changsheng Lin, Xiao Zhang, Ziqi Ye, Fang Zhou, Kaizong Huang, Shiting Zhu, Anliang Chen, Xueping Lisc-32815), IL-1β (1:1000, Santa Cruz Biotechnology, sc-12742), and TNF-α (1:1000, Santa Cruz Biotechnology, sc-52746). After TBST washes, membranes were incubated with HRP-conjugated secondary antibodies (1:5000, Proteintech,

(10) Phytochemical content, antioxidant and anti-inflammatory properties and wound healing effect of Chaiturus marrubiastrum: an in vitro and in vivo studyInflammopharmacologyFebruary 14, 2026Semih Bulut, Nasif Fatih Karakuyu, Emine Sarman, Ayse Sidal, Ahmet Kahraman, Mustafa Abdullah Yilmaz, Oguz Cakiralcohol series. The streptavidin-biotin complex peroxidase method was used. Primary antibodies for TNF-α (sc-52746, Santa Cruz, 1:200), Caspase-3 (sc-56053, Santa Cruz, 1:200), and VEGF (sc-57496, Santa Cruz, 1:200)

(11) Omega-3 Fatty Acids Attenuate LPS-Induced Acute Kidney Injury via Activation of AMPK/SIRT1/PGC-1α/NRF2/FOXO3 Signaling and Suppression of NF-κB-Mediated InflammationNutrientsFebruary 13, 2026Bindal Ahmet, Asci Halil, Kolay Oznur, Tepebasi Muhammet Yusuf, Kizilkaya Sinan, Ilhan Ilter, Bindal Tozlu Gulsum, Ozmen Ozlemprimary antibodies for 60 min at room temperature: TNF-α (Santa Cruz Biotechnology, Dallas, TX, USA sc-52746; dilution 1:100), Cas-3 (Abcam, Cambridge, UK, ab32351; dilution 1:200), and HSP70 (Abcam, Cambridge,

(12) Artesunate ameliorates experimental autoimmune uveitis by inhibiting the LCN2-STAT3 axis and suppressing microglial activationRedox RepFebruary 12, 2026Jingsai Gu, Qinyun Xia, Pingping Huang, Yilin Yuan, He Ren, Dihao Hua, Zhen Chen(interleukin-1β) (1:1000, Santa Cruz, sc-52012), TNF-α (tumor necrosis factor-α) (1:1000, Santa Cruz, sc-52746), IL-6 (interleukin-6) (1:1000, GeneTex, GTX110527), FTH1 (ferritin heavy chain 1) (1:1000, ZenBio,

(13) Lupeol restores dopaminergic function by suppressing glial activation in a Parkinson's disease mouse modelFront ImmunolFebruary 2, 2026Riaz Ahmad, Kyonghwan Choe, Hyun Young Park, Waqas Ahmad, Tae Ju Park, Myeong Ok Kim"Santa Cruz Biotechnology, United States",SC136548,1:1000 TNF-α,Mouse,WB,"Santa Cruz Biotechnology, United States",SC52746,1:1000 IL-1β,Mouse,WB,"Santa Cruz Biotechnology, United States",SC32294,1:1000 COX2,Mouse,WB,"Santa Cruz Biotechnology

(14) Osmotin-derived 9-amino-acid peptide alleviates α-synuclein and MPTP-induced glial cell activation mediated neuroinflammation, protecting dopaminergic neurons in Parkinson’s disease mice brainJournal of Biomedical ScienceJanuary 26, 2026Kyonghwan Choe, Muhammad Tahir, Min Hwa Kang, Hyun Young Park, Riaz Ahmad, Tae Ju Park, Myeong Ok Kim,Santa Cruz Biotechnology,SC33673,1:100 Iba-1,Rabbit,WB/IF,Abcam,Ab178846,1:1000/1:100 p-NF-кB,Mouse,WB,Santa Cruz Biotechnology,SC293132,1:1000 TNF-α,Mouse,WB,Santa Cruz Biotechnology,SC52746,1:1000 IL-1β,Mouse,WB,Santa Cruz Biotechnology,SC32294,1:1000 Nrf-2,Mouse,WB/IF,Santa Cruz Biotechnology,SC365949,1:1000/1:100 HO-1,Mouse,WB,Santa Cruz Biotechnology

(15) Manifold hepatoprotective actions of α-lipoic acid on metabolic function through redox regulation, inflammatory modulation, and anti-apoptosis after chronic sleep-deprived injuryFrontiers in NutritionJanuary 22, 2026Hung-Ming Chang, Hsing-Chun Lin, Ting-Yi Renn, Yu-Cheng Liu, Kai-Jung Yen, Chih-Kai Liao, Maria A. Tikhonova, Tamara G. Amstislavskaya, Sandeep Kumar Singh, Li-You Chen(1:2,000, Santa Cruz, sc-271803), NF-κB (p-65) (1:250, Santa Cruz, sc-8008), TNF-α (1:200, Santa Cruz, sc-52746), IL-6 (1:500, Bioss, bs-0782R-TR), IL-1β (1:100, Santa Cruz, sc-52012), COX2 (1:200, Santa Cruz, sc-376861),

(16) Pharmacological Inhibition of EZH2 by GSK ‐343 Attenuates Neuroinflammation in a Mouse Model of Spinal Cord InjuryJournal of Neuroscience ResearchJanuary 20, 2026Yinhui Kang, Deborah Mannino, Valentina Bova, Alberto Repici, Bulzomì Maria, Ahmed Hasan, Antonio Catalfamo, Jia Yang, Marika Lanza, Alessia FilipponeSanta Cruz Biotechnology); anti‐IL‐17 (1:500; sc‐374,218; Santa Cruz Biotechnology); anti‐TNF‐α (1:500; sc‐52746, Santa Cruz Biotechnology, Santa Cruz, CA, USA); anti‐CD45 (1:500; sc‐19597; Santa Cruz Biotechnology).

(17) High-entropy alloy Janus artificial enzymes for pH-gated sequential redox therapy of drug-resistant bacterial infectionNature CommunicationsJanuary 20, 2026Cong Han, Yongqi Wang, Shihuan Gao, Ting Wang, Huili Du, Jie Long, Weidong Tian, Mohsen Adeli, Liang Cheng, Zhi Liu, et al.Santa Cruz Biotechnology, USA, 1:100 dilution), IL-1β (sc-52012, Santa Cruz Biotechnology, USA, 1:200 dilution), TNF-α (sc-52746, Santa Cruz Biotechnology, USA, 1:200 dilution), VEGF (ab46154, abcam, USA, 1:200 dilution). The secondary

(18) Nivolumab and bevacizumab attenuate cisplatin-induced hepatic inflammation and apoptosis in ratsHepatology ForumJanuary 15, 2026Ogur Karhan, Sibel Turediperoxide (H2O2) for 10 minutes. Primary antibodies—TNF-α (Santa Cruz Biotechnology, Inc., Cat. No. sc-52746), IL-6 (Cat. No. sc-28343), VEGF (Cat. No. sc-7269), Bax (Cat. No. sc-7480), Bcl-2 (Cat. No. sc-7382),

(19) Cordyceps militaris Enhances Wound Repair Through Regulation of HIF-1α, TGF-β1, and SIRT1/Nrf2/HO-1 Signaling in Diabetic SkinLife (Basel)January 13, 2026Tzu-Kai Lin, Chia-Lun Tsai, Bruce Chi-Kang Tsai, Chia-Hua Kuo, Tsung-Jung Ho, Dennis Jine-Yuan Hsieh, Wei-Wen Kuo, Chih-Yang Huang, Pei-Ying Leesc-32322, Santa Cruz Biotechnology), anti-PCNA (1:50, sc-56, Santa Cruz Biotechnology), anti-TNF-α (1:50, sc-52746, Santa Cruz Biotechnology), anti-SIRT1 (1:50, sc-74465, Santa Cruz Biotechnology), anti-Nrf2 (1:100,

(20) Repositioning of Alogliptin to Mitigate Secondary Injury Induced by Repetitive TBI: Potential Role of its Antioxidant and Anti- Inflammatory EffectsJournal of Neuroimmune PharmacologyJanuary 13, 2026Hossam A. Raslan, Haidy E. Michel, Esther T. Menze, Amira A. El-Gazar(Santa Cruz Biotechnology, Inc; Cat. No sc-8008), anti-TNF-α (Santa Cruz Biotechnology, Inc; Cat. No sc-52746) and anti-HO-1 (Santa Cruz Biotechnology, Inc; Cat. No sc-390991) at a dilution of 1:200 and anti-Nrf2

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10/22/2019Time Course Effect of Chemoattractant on TNFalpha Expression Somasundaram RaghavanThis antibody works great for western blot. Highly recommended.

TNFα (52B83) Antibody

TNFα (52B83) Antibody from Santa Cruz Biotechnology, Inc.

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Santa Cruz Biotechnology, Inc.

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TNFα (52B83) Antibody from Santa Cruz Biotechnology, Inc.

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