Time Course Effect of Chemoattractant on TNFalpha Expression

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Santa Cruz Biotechnology

Product Name:

TNFα Antibody

Catalog Number:

sc-52746

Clone Number:

52B83

Tumour Necrosis Factor alpha (TNF alpha), is one of the most important inflammatory cytokine produced by macrophages and monocytes. It is responsible for various process such as signaling events within cells, necrosis and apoptosis. Here we checked the role of chemoattractant on TNFalpha.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lysate

Primary Incubation

1:1000 dilution prepared in 1x TBST

Blocking Agent

5% milk with 1x TBST

Secondary Incubation

1:4000 dilution prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

Observed time dependent increase of TNFalpha by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

This antibody is very good for western blot experiment

The Bad

None

The Bottom Line

This antibody works great for western blot. Highly recommended.

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